在后

Methods After brain death of the donor was confirmed, the whole donor arm was sterilized with 3%iodine tincture and 75%ethyl alcohol and strapped with sterile towels. A circular incision 5 cm above the elbow was made, and the brachial artery was dissected and catheterized for perfusion of UW solution at 4℃. The arm was amputated with the brachial artery still in continuity and catheterized.

方法在供者被宣布脑死亡后，将拟取肢体用3%碘酒、75%酒精常规消毒，从肘关节以上5 cm平面作环形皮肤切口，游离肱动脉后插管，用4℃ UW液灌注后行前臂离断，再灌洗至回流液清亮后拔管，肢体作标记后用无菌敷料包裹、塑料袋封装，装入放置冰袋的保温箱中快速运输。

Methods 38 cases with 53 hips of ANFH treated with the method of thrombolysis and opening catheterizing and perfusing medial and lateral circumflex arteries.Those drugs are Urokinase 200 thousand Units,Danshen Root 20 ml and Ligustrazine 20 mg. Retention catheter was held in the target vessels and infused the same drugs besides Heparin 4500u in 3 days. To evaluate therapeutic effect ,the DSA of the femoral artery and x-ray plain film and/or CT/MRI of the femoral head were performanced. Results All cases were followed up 1~16 (mean 9.5) months.

方法对38例ANFH患者的53髋先常规行股动脉DSA，后在股骨头的供血动脉旋股内、外侧动脉内灌注溶栓、扩血管药物，即在每支血管内注入稀释后的尿激酶 20万u、丹参10 ml及川芎嗪20 mg；术后保留导管于动脉内，经导管每日注入同剂量的药物及肝素钠4500 u，连用3天后拔管，治疗后复行股动脉DSA检查及行股骨头X线平片及/或CT、MR随访以评估疗效。

Each variety was sown into three blocks.They were harvested at 79～83d(MS1), 86～89d(MS2),93～96d(MS3) and 98～100d(MS4) after seeding and ensiled with four different ensiling treatments(R group:adding lactobacillus,3×10~9cfu/t,E group: adding cellulase 2×10~4u/kgDM,xylanase 6×10~5 thousand u/kgDM andβ-glucanase 5×10~5 u/kgDM,R+E group:adding lactobacillus 3×10~9cfu/t and enzyme preparation as E group together,group:adding no additives but carrier).The whole crop rice were pestled by mallet and then chopped into 3cm～5cm after they were harvested and then ensiled in a 6L polyethylene pot and sealed.

为探索全株青贮饲料稻的适宜品种、适宜收获时期和适宜青贮处理方法及其品质评价，2006年，选择饲料稻湘早籼24、湘丰早119和浙辐802，采用相同的栽培技术，种植在同一大田，每个品种设三个重复小区，分别在四个不同成熟阶段(MS1：播种后79～83d；MS2：播种后86～89d；MS3：播种后93～96d；MS4：播种后98～100d)收割，采用四种青贮添加剂处理(R组：添加乳酸菌3g/t；E组：添加纤维素酶2万u/kgDM、木聚糖酶60万u/kgDM和β-葡聚糖酶50万u/kgDM；R+E组—添加乳酸菌+复合酶，其中乳酸菌添加量同R组，复合酶添加量同E组；K组—不添加任何添加剂)。

The fire coal falls on the chain fire grates from coal: bucket and hen goes into the furnace tank for burning, and the flame passes through the upper back arch of the combustion chamber and back snloke chamber and then through the second fire pipe Io the

燃料煤自煤斗落在链条炉排面上，进入炉膛燃烧后，火焰经过后拱上部燃烧室和后棚管烟室后进入二回程烟管到前烟箱，再由前烟箱折回第三回程管束后，通过热管空气预热器、多管除尘器，然后由引风机抽引经过烟囱排出。

Measure the items on before and after the two courses of treatment respectively (one course for 15 days) as follows:①Syndromes Investigation: to adopt the scoring methods of symptoms and signs with the certain standard scoring table of symptoms and classifying table of signs for the patients, on the day before and after the two courses of treatment respectively,②Signs Investigation: the mucoma visibility, swelling or hyperemia conditions, or gloyp and polypoid change in the inferior and middle nasal conchas, nasal septum, etc with a nasal endoscope on the day before and after the two courses of treatment respectively,③Test of the interferon-γ、interleukine-4(IL-4) in serum of patients by the method of enzyme linked immunosorbent assay on the previous day before and after the two courses of treatment respectively.

治疗组在治疗前、两个疗程结束后分别进行以下项目的观察：①临床症状的评定：治疗前及两个疗程结束后当天对患者鼻痒、鼻塞、流涕、喷嚏症状的程度进行评分；②体征的评定：治疗前及两个疗程结束后当天采用鼻额镜肉眼观察患者下鼻甲鼻粘膜颜色、肿胀情况，及中鼻甲是否可见，有否息肉样变等；③治疗前及两个疗程结束后一天清晨空腹抽取患者肘静脉血4ml离心后，采用酶联免疫吸附试验检测患者血清IFN-γ、IL-4的含量。

RESULTS:①A total of 178 injected and survived zygotes were replanted. Thirteen mice were born with the birth rate of 7%.

结果：①178只注射pcDNA3.1-mutant LMNA后的存活受精卵移植至假孕鼠输卵管后，出生13只子代鼠，出生率只有7%；58只注射pcDNA31 -mutant LMNA后的存活受精卵移植到假孕鼠输卵管后，在移植后12~15 d的受体母鼠子宫内收集到1只活胚和5只死胚。

In the reforming cryopreserving method, the fetal liver tissues were permeated and equilibnzed in cryoprotectant A and B for 20 minutes, respectively, and then put into liquid nitrogen quickly after cold equilibrium at liquid nitrogen steam for 5 minutes.

改良冻存法：胎肝组织在A液、B液中分别渗透平衡20min后，在冷冻保护液B液中经液氮蒸汽冷平衡5min后迅速入液氮冻存，1周后复苏，入DMEM培养液37℃孵育1h，胶原酶消化胎肝组织，分离、收集消化后的胎肝细胞培养观察。

The mainly conclusions in our research are as flowing: tungsten trioxide powders with 100 nm primary particle are obtained by spray drying,calcination and wet milling process, and an average size of quadric particle composed of agglomerated particles is 0.64 μm; tungtsen powders with 39 nm grain size and 60 -100 nm primary particle are produced directly from previous tungsten trioxide using one step reduction in hydrogen at 700℃, and an average size of quadric particle of tungsten powder is 2.91 μm; tungsten trioxide and copper tungstate compound powders with 100 nm - 200 nm primary particle are produced using ammonia metatungsten and copper nitrate as raw materials by spray drying,calcination and wet milling process;the compound powders are transformed completely into tungsten and copper compound powders by reduction in hydrogen at 700℃,in which tungsten grain size is 59 nm and copper grain size is 51 nm; primary particle size of compound powders is 80 - 120 nm,and an average size of agglomerated quadric particle is 1.86 μm; tungsten nitride powders with 35 nm grain size are prepared from tungsten trioxide powders by nitrogen treatment thoroughly in pure ammonia at 650℃, and an average size of agglomerated quadric particle is 0.64 μm in normal temperature.

研究结果表明：采用喷雾干燥—焙烧—球磨工艺可以制备出粒度约为100nm的WO_3粉体，它们在团聚后形成的二次颗粒平均粒度为0.64μm；采用一步直接氢还原方法可在700℃下从上述WO_3粉体制备出晶粒尺寸为39nm的、一次颗粒粒度为60-100nm的W粉体，其二次颗粒的平均粒度为2.91μm；以偏钨酸铵、硝酸铜为原料，采用喷雾干燥—焙烧—球磨工艺可制备出一次粒度为100-200nm的WO_3和CuWO_4混合粉体；采用氢还原工艺可在700℃下将这种粉体完全转变为W、Cu复合粉体，其中W的平均晶粒粒尺寸为59nm，Cu的平均晶粒尺寸为51nm；复合粉体的一次颗粒尺寸为80-120nm，在常温下团聚后形成的二次平均粒度为1.86μm；采用纯氨氮化工艺可以在650℃下由WO_3粉体制得WN，其晶粒尺度为35nm，在常温下团聚后的二次平均粒度为6.4μm。

The ethanol content in fruit flesh of'Starking'apple, 'Yar'pear and'Shingo'pear increased in early storage with ethanol-applied concentration increased and decreased later. At the end of the storage for 5-6months, there was no difference in ethanol concentration in fruit flesh in all treated apple or'Yar'pear treated with 2 and 4ml/kg fruit of ethanol and control, but that in 6ml/kg fruit of ethanol treated'Yar' pear was higher than that in control.

红星苹果、鸭梨和新高梨经乙醇处理后，果肉中乙醇含量呈先升后降趋势，果实内积累乙醇浓度与乙醇处理浓度显著正相关。2、4、6ml/kg乙醇处理的红星苹果在贮藏5、6个月后，果实中的乙醇含量与对照无差异；2和4ml/kg乙醇处理的鸭梨在贮藏5、6个月后果肉中的乙醇含量与对照无显著性差异，但6ml/kg的乙醇处理果实中的乙醇含量却显著高于对照；相同的乙醇处理浓度使新高梨果实中积累更高的乙醇含量。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示：(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散，但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率，而且可加强卵丘细胞的扩散；(3)在现有实验条件下，在mTCM和mNCSU中添加10％pFF与在mNCSU中添加10％FCS相比可获得较高的猪卵母细胞核成熟率；(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样，但对体外受精后的早期胚胎发育影响不明显；(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。