在后

The article in recent years the research progress of disease of oral cavity of TP prevention and cure makes one overview. The compositive TP of the basic research 1.1 TP of 1 TP is the floorboard that phenolic apperception of a kind of many hydroxyl closes matter, 25% what hold tea dry weight about, its are main component includes to express gallnut of gallnut catechu element gallnut of element of catechu of gallnut of acerbity ester, watch, watch catechu element is acerbity element of catechu of ester, watch, express gallnut of gallnut catechu element among them acerbity ester content is highest, occupy the 80%[1] of catechu element about, fighting oxidation with its unique element structure, fight the position with fight the respect such as tumor to have choppy, significant. The 1.2 TP absorption in oral cavity and metabolization Lee [2] is measured normally inside a hour after healthy person is chewing green tea Xie Huo contains juice of gargle green tea, content of the TP in saliva is very high, after observing green tea passes salivary enzymatic hydrolyze, TP is slow release and in oral cavity;Yang of local play action [the salivary level after 3] checked 6 volunteers to drink tea, chroma of the TP in making clear the saliva after drinking tea as a result is serous TP pH indicator 2 times, and the salivary TP concentration that contains gargle tea solution a few minutes to be able to produce higher level, and TP can be absorbed through oral cavity mucous membrane.

本文就近年来TP防治口腔疾病的探究进展作一综述。1 TP的基础探究1.1 TP的组成TP是一类多羟基酚类化合物的总称，约占茶叶干重的25%，其主要成份包括表没食子儿茶素没食子酸酯、表没食子儿茶素、表儿茶素没食子酸酯、表儿茶素等，其中表没食子儿茶素没食子酸酯含量最高，约占儿茶素的80%[1]，并以其独特的分子结构在抗氧化、抗突变、抗肿瘤等方面占有重要的地位。1.2 TP在口腔中的吸收和代谢Lee等[2]测得正常健康人在咀嚼绿茶叶或含漱绿茶液后的一个小时内，唾液中TP含量很高，并观察到绿茶通过唾液酶水解后，TP缓慢释放并在口腔局部发挥功能；Yang等[3]检查了6位志愿者饮茶后的唾液水平，结果表明饮茶后唾液中TP浓度是血浆TP浓度的2倍，而含漱茶溶液几分钟即可产生更高水平的唾液TP浓度，且TP可通过口腔黏膜吸收。

This new genus is rather similar to Hemeroscopus Pritykina,1977, but differs from the latter in the following characters:(1) hindwing anal loop is smaller, only with 6-7 cells;(2) Rspl is absent;(3) the hindwing vein CuAa is curved and has five distinct posterior branches;(4) the forewing MP is not shortened, reaching the posterior wing margin slightly beyond the level of the nodus;(5) pterostigmata more distinctly braced;(6) the hindwing area between MP and CuAa is narrow, with only one row of cells near the discoidal triangle.

新属与巴依萨昼蜓HemeroscopusPritykina，1977在形态上比较相似，但在演化程度上更为原始，主要体现在下面几个方面：(1)后翅臀套较小，仅有 6～ 7个翅室；(2 )径增脉缺；(3)后翅CuAa脉弯曲，带有 5个明显的后分支；(4 )前翅MP脉不显短，终止于翅后缘近翅结处；(5 )翅痣下有一个明显的支脉；(6 )后翅CuAa和MP域基部较窄，在三角室下方仅有 1排翅室。

3During 15 to 30 days after anthesis,the CHL content of the wheat flagleaf decrease promptly,and there are significant differences between CTW and WTW.Thedecreasing rates of the CHL of CTW are lower than that of WTW.Similar results are foundin the WSP content of flag leaf.The MDA content of the wheat flag leaf increase exponen-tially along with the DAA.

开花后15～30天，小麦旗叶叶绿素含量迅速下降，冷型小麦旗叶CHL分解缓慢、CHL稳定时间长；小麦开花后旗叶可溶性蛋白质含量迅速下降，不同温型小麦的下降速度有别，冷型小麦下降速度慢，结果导致从开花后15天开始，冷型小麦WSP含量高明显高于暖型小麦；小麦旗叶丙二醛含量在开花后随天数的增加而呈指数增长，在整个籽粒形成和灌浆期，冷型小麦MDA含量均低于暖型小麦，从花后12天开始，不同温型小麦之间MDA含量的差距越来越大。

The results showed that Weipa ore is gibbsite and its leaching efficiency is high when digested at higher than 200 ℃,so applicable to sweetening ore in digestion process,under conditions that the sweetening process is controlled in the range of 195～200 ℃ for 15～20 min and the dosage of Weipa .

对澳大利亚韦帕矿的溶出性能及以韦帕矿为后加矿的增溶溶出技术进行了实验研究。考察了增溶温度，增溶时间，后加矿加入量对溶出效果的影响，同时对后加矿增溶溶出的矿浆性能进行了对比性研究。结果表明：韦帕矿为三水铝石矿，在200℃以上溶出时，浸出率较高，适宜作为增溶溶出过程的后加矿。以韦帕矿为后加矿，其适宜的增溶溶出条件为：增溶温度195~200℃，增溶时间15~20 min，后加矿的质量分数为15%~20%。后加矿增溶溶出矿浆具有优良的沉降压缩性能

Usually there are two syllables in a light tone-phrase and the law of pitch-changing is achieved by comparing a lot of phonetic figures·Generally speaking, when the first syllable is of a falling-rising tone, the pitch of light tone is of a half falling-rising tone.

通常前一个音节为上声时，其音高表现为半上；轻声音节在阴平后为41调值，在阳平后为51调值，在上声后为33调值，在去声后为10调值。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

Results: 1. The value of all experimental indexes decreased significantly after heat stress, and reached the lowest at the time 2-8 hours after heat stress. This indicates that the immunity of the mice is damaged to the largest extent at 2-8 hours after heat stress. 2. L-arginine supplementation with appropriate dose could remit the acute atrophying of thymus and spleen tissue caused by heat stress. 3. After L-arginine supplementation with appropriate dose, the lymphocyte proliferation, the level of concentration of IL-2 and * expression of IL-2R raised in the group with room temperature; in the heat stress group the level of three indexs decrease significantly. Furthermore, the decrease of the group given 1 .Smglg.bw L-arginine is the smallest. This result indicates that the concentration of 1 .Smglg.bw L-arginine supplementation could remit the depressment of the immunity caused by heat stress. 4. The [Ca2~] in activated thymocytes of the group with L-arginine -2- supplementation is significantly higher than that of the group with water supplementation. This indicates that L-arginine supplementation could protect thymoeytes of mouse under heat stress. We also found that the fluorescence intensity of [Ca2~] in activated thymocytes of the group given 1 .Smglg.bw L-arginine is the highest.

结果：1、热应激后小鼠的各项免疫指标均有显著性下降，其中在2.8小时降到最低，提示在这个时间段小鼠的免疫功能受到最大损害；2、适量补充精氨酸有助于缓解热应激导致小鼠胸腺和脾脏的急性萎缩；3、常温组，补充适量精氨酸后小鼠淋巴细胞增殖活性、IL-2的浓度及IL-2R的表达均有显著性上升，而热应激后三指标均显著降低，其中在精氨酸给予浓度为1.5mg/g.bw时三指标的降低程度最少，提示该浓度可最大程度缓解热应激对小鼠免疫功能的抑制；4、补充精氨酸组的活化胸腺细胞［Ca~(2+)］较给水组有显著性上升，说明精氨酸对热应激小鼠胸腺细胞具有保护作用，并发现在精氨酸给予浓度为1.5mg/g.bw时活化胸腺细胞的胞浆钙离子荧光强度达到最大；5、热应激后小鼠血清NO的浓度均有不同程度的上升，补充精氨酸后血清NO上升更加明显，但并非随精氨酸给予浓度的增加而增加。

The higher the concentration, the bigger the physiological index. 3. The Ether extract, the n-BuOH extract, the acetone and the chloroform extract treatment after chilling stress and restored the growth, the content of protein, the content of chlorophyll, SOD、POD and CAT activities were higher compared the control, the content of MDA was lower compared the control.

经前胡乙醚、正丁醇、丙酮和三氯甲烷提取物质喷雾处理的幼苗在低温处理36小时后，可溶性蛋白质含量、SOD、POD、CAT活性均下降，在低温处理恢复生长后，SOD、POD、CAT、可溶性蛋白和总叶绿素含量均比低温对照幼苗高，MDA含量比低温对照幼苗低；在低温处理后及恢复生长后各生理指标随浓度的增加呈现出先升高后降低的趋势。

In control, however,the weak expression ofCY3 mRNA can be found after 48 hours of chilling and showed little increase at the third day instress.

非逆境条件下，IPT对秧苗根系中mRNA的表达差异与对照相比没有很大的变化；低温胁迫3天后，IPT对根系中mRNA的表达影响很大，既能增强某些基因的表达，又能抑制另一些基因的表达；用DDRT-PCR技术，寻找到5个低温胁迫下在对照和IPT处理中差异表达的基因，其中CY3片段与水稻CDPK1和CDPK2的mRNA有很高的同源性，初步断定CY3在植株的抗逆反应中可能具有与CDPKs相似的功能；在Genbank中没有检索到与其它4个片段同源性比较高的已知序列；IPT处理中CY3表达量在低温处理24hr就有明显升高，以后随胁迫时间的延长其表达量不断加强；恢复常温后，CY3表达继续增加，在恢复第14天开始下降，下降速度缓慢，IPT对CY3的影响一直延续到苗末期，对照中CY3在低温胁迫48hr才有微弱表达，72hr后表达量并未增加许多；恢复常温后第4天就开始下降，很快降到最低。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。