在后

While the adsorptions of 1-propanol and 1-Br-propane at 100℃ give rise to 1-propoxy species, which mainly converted to propanal at higher temperature. IR spectrum arising from the interaction of propene with the catalyst at 100 ℃ shows the IR bands of allyl alcoholate and isopropoxy species. As the temperature was increased to 150℃, the bands ascribable to the adsorbed acetone and acrolein were observed. Based on these results, it is suggested that isopropoxy species is one of the intermediates for SOP to acrolein over MoPO/SiO〓 catalyst.

2原位IR光谱实验结果表明：200℃时丙烷/O〓混合气在催化剂表面吸附后的IR谱与同温度下异丙醇吸附的IR谱相似，均检测到吸附态丙酮物种和丙烯醛物种的IR谱峰；溴代异丙烷和异丙醇在催化剂表面吸附后均转化为异丙氧基，异丙氧基主要转化为丙烯和丙酮，丙烯进一步转化为丙烯醛；溴代正丙烷和正丙醇在催化剂表面吸附后均转化为正丙氧基，正丙氧基主要转化为丙醛；丙烯在催化剂表面吸附后转化为σ-氧烯丙基和异丙氧基，升温后σ-氧烯丙基转化为丙烯醛而异丙氧基转化为丙酮。

While the adsorptions of l-propanol and 1-Br-propane at 100 癈 give rise to 1-propoxy species, which mainly converted to propanal at higher temperature. IR spectrum arising from the interaction of propene with the catalyst at 100 癈 shows the IR bands of allyl alcoholate and isopropoxy species. As the temperature was increased to 150 癈, the bands ascribable to the adsorbed acetone and acrolein were observed. Based on these results, it is suggested that isopropoxy species is one of the intermediates for SOP to acrolein over MoPO/SiO2 catalyst.

原位IR光谱实验结果表明：200℃时丙烷／O_2混合气在催化剂表面吸附后的IR谱与同温度下异丙醇吸附的IR谱相似，均检测到吸附态丙酮物种和丙烯醛物种的IR谱峰；溴代异丙烷和异丙醇在催化剂表面吸附后均转化为异丙氧基，异丙氧基主要转化为丙烯和丙酮，丙烯进一步转化为丙烯醛；溴代正丙烷和正丙醇在催化剂表面吸附后均转化为正丙氧基，正丙氧基主要转化为丙醛；丙烯在催化剂表面吸附后转化为σ-氧烯丙基和异丙氧基，升温后σ-氧烯丙基转化为丙烯醛而异丙氧基转化为丙酮。

The result shows, grass of Sikkim small hole is temporary decline Yan period with permanent decline water content of Yan period soil is respectively 7.06% be in temporary decline with 6.20%; the Sikkim of Yan period is small after water of Kong Cao answer bibulous and rapid, decline Yan condition is eliminated gradually, appearance of aftertreatment group of answer water 6 H and contrast active of basic consistent;SOD is during threatening processing with markedder than notting have to taking a picture change, the ascendant;CAT that there is certain range after answer water and POD active are in threatening during after rising first, drop, all remarkable prep above contrasts level, and the active;MDA content that still keeps taller after answer water as the extension of time of arid and threatening processing mushroom, to threatening the 8th climate reachs maximum, content of the MDA in the lamina after answer water drops quickly, arrive the 5th day after answer water, content of the MDA in threatening group lamina is adjacent already contrast level.

结果显示，锡金微孔草暂时萎焉期和永久萎焉期土壤含水量分别为7.06%和6.20%；处于暂时萎焉期的锡金微孔草复水后吸水迅速，萎焉状态逐渐消除，复水6 h后处理组外貌和对照基本一致；SOD活性在胁迫处理期间和对照相比无显著变化，复水后有一定幅度的上升；CAT和POD活性在胁迫期间先上升后下降，均显著高于对照水平，且复水后仍然保持较高的活性；MDA含量随着干旱胁迫处理时间的延长而迅速增加，到胁迫第8天时达到最大值，复水后叶片中MDA含量迅速下降，到复水后第5天，胁迫组叶片中MDA含量已接近对照水平。

The results were as follows: Being infected virus, the electrolyte exosmosis rate of tobacco leaf was increased , while the increase rate of the leaf being injected ion was less than that of the control. The activities of SOD?

结果表明：病毒侵染后烟草叶片电解质外渗率增大，但离子注入后烟草的电解质外渗率增幅小于对照；病毒侵染后烟草叶片的超氧化物歧化酶、过氧化物酶、硝酸还原酶的活性及叶绿素含量均有所下降，但离子注入后烟草的上述酶活降幅较小；CAT活性在接毒后呈先低后高的变化，而对照在接毒后 10天该酶活性高出离子注入烟草 2倍多，随后剧降，到接毒 2 5天时仅为离子注入处理烟株 1/ 2。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

The comparison was made of amplitudes of the postsynaptic potential evoked in AP cells by the stimulation of T, P and N cells. The results showed that for each type of T, P and N cell the contralateral member of the pair evoked larger synaptic potentials in the AP cell that did its ipsilateral homologue and the T, P and N cell with a dorsally situated receptive field produced a larger synaptic input on the AP cell that its counterpart with a ventrally situated receptive field.

分别比较T、P和N细胞在AP细胞上引起的突触后电位的幅度，可见对侧T、P和N细胞在AP细胞上引起的突触后电位幅度比同侧的T、P和N细胞在AP细胞上引起的突触后电位幅度大；具有背部感受野的T、P和N细胞在AP细胞上引起的突触后电位的幅度比具有腹部感受野的T、P和N细胞在AP细胞上引起的突触后电位的幅度大。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

After HRP application to the rostral area (n=10), quite a number of labelled cells were distributed at the ventrolateral part of the nucleus of trapezoid body, the lateroventral periolivary nucleus and the retrotrapezoid nucleus. After HRP application to the caudal area (n=10), HRP labelled cells were mainly located at the lateral reticular nucleus and the ventral part of at the lateral paragigantocellular nucleus LPGi. After HRP application to the intermediate area (n=6), only 8HRP labelled cells were observed at the in 6rats. After HRP application to the control area (n=4), only 6HRP labelled cells were found at the rostral ventrolateral reticular nucleus.

大鼠延髓腹外侧表面的头端化学感受区表面敷贴辣根过氧化物酶后，在延髓斜方体核、外周橄榄腹外侧核和斜方体后核均有HRP标记细胞存在；于尾端化学感受区表面敷贴HRP后，在延髓的巨细胞旁外侧核、外侧网状核和舌下神经核有HRP标记细胞存在；于中间区表面敷贴HRP后，在6只大鼠的延髓内部区域仅观察到8个HRP标记细胞；于对照区敷贴HRP后，在4只大鼠的延髓内部区域仅观察到6个HRP标记细胞，散在地分布在头端腹外侧网状核。

With regards to the learning motive of music, after excluding the pretest influence, it is the finding of the study that the students belonging to the experimentation group score higher in the areas of "Total score","Internal goal orientation","Work value","Controlled belief","Expectation to succeed" and "Test on anxiety" than the control group, amongst which "Work value" reaches significant difference on the measurement chart; of all the students scoring high on "Selective attention" during pretest, the experimentation group scores higher than the control group on posttest scores; regarding the students with lower pretest scores, the control group performs better than the experimentation group; of the students with higher pretest scores on "Discrete attention", the posttest scores of experimentation group are higher than those of the control group; regarding students with lower pretest scores, the control group performs better than the experimentation group; on "Sustained attention", the posttest scores of the experimentation group are higher than those of the control group; on the "Total score" of multidimensional attention measurement, the posttest scores of the experimentation group are higher than those of the control group.

研究结果发现，在音乐学习动机方面，经排除前测影响后，实验组学生在「总分」及「内在目标导向」、「工作价值」、「控制信念」、「期望成功」、「测试焦虑」等分量表上皆高於对照组，其中在「工作价值」分量表上达到显著性差异；在「选择性注意力」前测分数较高学生中，后测成绩为实验组显著优於对照组，对前测分数较低学生而言，则是对照组显著优於实验组；在「分离性注意力」前测分数较高学生中，后测成绩为实验组显著优於对照组，对前测分数较低学生而言，则是对照组显著优於实验组；在「持续性注意力」方面，后测成绩为实验组显著优於对照组；在多向度注意力量表「总分」方面，后测成绩为实验组显著优於对照组。

There was significant difference in signal intensity between the cell concentration group and unlabelled cell group during 2 weeks. And significant difference occurred in the 5×105 SPIO-labeled cells per milliliter medium group and unlabelled cell group during 3 weeks.3. In kidneys of ischemia-reperfusion injury,the labeled BMSCs were demonstrated as signal intensity loss in renal medulla on T2*WI sequence in days 1,3,and5.The signal intensity loss was visualized up to 8 days after transplantation,and the signal intensity in the third day was the lowest. Histological analyses showed that most Prussian blue staining-positive cells were well correlated with the area where a signal intensity loss was observed in MRI.

在此细胞浓度的基础上，标记后细胞信号在T2*WI序列的降低在2w后失去统计学意义；而在细胞浓度为5×105/ml时，标记3w后信号在T2*WI序列的降低才失去统计学意义。3缺血再灌注肾损伤家兔细胞移植后第1、3、5天肾脏髓质区信号强度明显下降，至移植后第8天信号改变不明显，其中第3天信号改变最为明显；组织学分析见绝大多数普鲁士蓝染阳性标记细胞分布于肾小管周围，与MRI信号强度下降区域基本一致。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。