在后

A self-drilling anchor (10) for use in a friable material (1) comprises a body (12) having an axis (13), a flanged rear end (14), a drilling front end (16) and a generally cylindrical portion (18) therebetween having an outer surface (20) with a thread (22) disposed thereon, wherein the body forks, beginning at a predetermined distance from the flanged rear end, into a first leg (24) and a second leg (26), the first leg extending forwardly into a drilling tip (28) and having a generally rearward facing shoulder (30) angled obtusely outwardly with respect to the axis, wherein the body (12) has an axial bore (32) for receiving an elongate fastener (2), the axial bore (32) extending substantially through the flanged end (14) and the generally cylindrical portion (18) and leading to the generally rearward facing shoulder (30), wherein the anchor (10) has a drilling mode wherein the second leg (26) nests behind the generally rearward facing shoulder (30) of the first leg (24), and an anchoring mode wherein the legs are pivoted apart from one another.

一种用于易碎材料(1)中的自攻螺钉(10)，包括：主体(12)，其具有中心轴(13)、带有凸缘的后端部(14)、用于钻孔的前端部(16)和位于所述后端部和前端部之间的通常为圆柱状的部分(18)，所述圆柱状部分(18)的外表面(20)上布置有螺纹(22)，其中主体从距离带有凸缘的后端部的预定的距离处开始分叉为第一腿(24)和第二腿(26)，第一腿向前伸入钻孔尖端(28)，并且具有相对于中心轴向外成钝角的通常面向后的肩部(30)，主体(12)具有容纳细长状紧固件(2)的轴向孔(32)，轴向孔(32)基本延伸穿过带有凸缘的端部(14)和通常为圆柱状的部分(18)，并且通向通常面向后的肩部(30)，螺钉(10)具有钻孔状态和固定状态，在钻孔状态，第二腿(26)套在第一腿(24)的通常面向后的肩部(30)之后，并且在固定状态，腿彼此转动分离。

The effects of different plant densities on flag leaves chlorophyll content,SOD activity and MDA content after anthesis in stronggluten wheat interplanted in paddyfield were studied.

本试验在江苏淮北地区设计不同密度试验，研究其对稻田套播强筋小麦花后剑叶叶绿素含量、SOD酶活性和MDA含量的影响，结果表明：随花后生育进程推移，叶绿素含量呈下降趋势，且随密度增加，下降幅度增大；花后单茎干物质积累量和单茎籽粒产量亦随密度增加而下降，二者呈极显著负相关；随花后生育进程推移，剑叶SOD酶活性呈单峰曲线变化，可用二次曲线方程模拟，在花后1416 d达峰值，且随密度增加峰值出现时间提前，峰值数值呈先上升再下降的变化特征；随花后生育进程推移，剑叶MDA含量呈上升的变化特征，且随密度增大，数值变大；花后35 d剑叶SOD酶活性与花后单茎干物质积累量和单茎籽粒产量呈极显著正相关，而MDA含量与花后单茎干物质积累量和单茎籽粒产量则呈显著或极显著负相关。

Results: During the development of spinal cord. NIDD mRNA was highly expressed at embryo 16 d and at postnatal 1 d, and co-localized with nNOS in the undifferenti ated ventral horn, as well in white matter. And then it decreased significantly till postnatal 12w. nNOS mRNA was highly expressed from postnatal 1 d to 3 d. The high expression of NIDD mRNA appeared at the 8 hour after spinal cord injury, and co-localized with nNOS in neurons of ventral horn, intermedial zone, the area around central canal and dorsal horn, and recovered to the normal at dry 7 day after SCI.

结果：大鼠发育过程中，胚胎16 d的大鼠脊髓中可见NIDD mRNA的高表达，在生后1d，与nNOS共表达于尚未分化成熟的前角，在白质也见NIDD的阳性信号成年后呈低表达；nNOS mRNA于生后1~3 d出现表达高峰；脊髓损伤后NIDD mRNA表达明显增多，在8 h到达高峰，分布于脊髓前角，中间带、中央管周围及后角nNOS阳性的神经元，7 d恢复至正常水平；nNOS mRNA在损伤后8 h达到高峰，1 d降低至正常水平。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

While the tissue spaces surrounding a few blood vessels wasAl and Fg positive,no Al or Fg positive cells were observed.In antemortem injurygroup,diffuse subarachnoid hemorrhage,cerebral edema,swelling or pyknotic neu-rons could be observed.The axons showed irregular swelling and disconnection at1～3h,marked swelling and disconnection at 6h,and retraction ball at 15h whichwas more remarkable at 24h after injury.The space between myelin sheaths andaxons was increased at 3～6h after injury.Tortuous and wavelike myelin sheathswhich adhered on axons incompletely,or even peeled off could be found from 15hto 24h after injury.Perinuclear lysis of Nissl bodies began at 24h after injury.Thenumber of GFAP positive cells in cerebrum and brain-stem increased significantlyfollowed by decrease,and then increased again,but the time courses of the changesin different areas of brain were not same.Al and Fg positive neural cells,mainlysurrounded blood vessels,with diffuse or peripherally distributed positive matter incytoplasm could be observed at 0.5h after injury.The number of Al or Fg positivecells and the intensity of immunoreaction increased with the time of injury.The areaof SYN positivity in medulla oblongata and pons decreased notably 3～6h afterinjury,then return to normal levels and continued to 24h after injury.

生前损伤组，可见广泛蛛网膜下腔出血，脑组织水肿，神经细胞肿胀，晚期神经元固缩；伤后1～3h见部分神经轴突不规则增粗、断裂，伤后6h断端膨大，伤后15h可见收缩球，至伤后24h更为明显；伤后3～6h可见部分神经髓鞘与轴突之间的间隙增宽，伤后15h髓鞘明显曲折，不完全附着在轴突两侧，甚至剥脱，持续到伤后24h；核周尼氏体减少在伤后24h才开始出现；同一部位的GFAP阳性细胞数目随损伤时间发生改变，先增多（最早在伤后0.5h），达到高峰后减少，其后又有增多趋势，但不同部位的GFAP阳性细胞数目增减的时间过程不尽相同，同时，大脑中的GFAP阳性细胞数目也有改变；伤后0.5h，可在脑干组织中见到Al和Fg阳性神经细胞，主要位于血管周围，阳性物在胞浆中呈弥散性分布，但部分细胞的阳性物仅分布于靠近胞膜的胞浆中而呈环状，随损伤时间延长，阳性细胞数目增多，反应强度增加；伤后3～6h，延髓及桥脑中的SYN阳性物面积减少，其后恢复到正常水平，并持续到伤后24h。

Among the 45 neurons generating phasic firing, 8 (17.78﹪) neurons could still be induced phasic firing after treatment with 1×10-2 μg/mL SVHRP and 37 (82.22﹪) neurons had no responses to the stimulation. The AP firing of neurons was dramatically different after treatment with SVHRP (P＜0.01, n=45). Among the 7 repetitive firing neurons, all of them could only generate 1 or 0 AP instead of repetitive firing when SVHRP was applied. The number of APs was 14.57±1.00 and 0.57±0.20 before and after SVHRP treatment (P＜0.01, n=7). The AP rheobase was (75.10±8.99) pA and (119.85± 12.73) pA before and after 1 × 10-4 μg/mL SVHRP application, respectively (P＜0.01,n=8). The AP threshold was increased from (-41.17±2.15) mV to (-32.40±1.48) mV after 1×10-4 μg/mL SVHRP treatment (P＜0.01,n=8). The peak amplitude of AP was (68.49±2.33) mV for the neurons before treatment with 1×10-4 μg/mL SVHRP and (54.71±0.81)mV after treatment (P＜0.01, n=8). These results showed that SVHRP could decrease the AP firing frequency, increase the AP rheobase and threshold, but decrease the AP peak amplitude of hippocampal neurons.

在产生位相放电的45个细胞中，有8个细胞在SVHRP处理后仍可以诱发出位相放电(占17.78﹪)；37个细胞在SVHRP处理后无法诱导出位相放电(占82.22﹪)，SVHRP处理后动作电位的产生与处理前相比，有显著差异(P＜0.01，n=45)；在产生重复放电的7个细胞中，在1×10-2μg/mL SVHRP作用后均不能再次诱发出重复放电，而是产生一个动作电位或不再产生动作电位，药物处理前产生的动作电位个数为14.57±1.00，SVHRP处理后产生动作电位的个数为0.57±0.20，二者之间有显著性差异(P＜0.01，n=7)。1×10-4 μg/mLSVHRP处理后，诱发动作电位产生的基强度由(75.10±8.99)pA增加到(119.85±12.73)pA(P＜0.01，n=8)；阈电位由(-41.17±2.15)mV升至(-32.40±1.48)mV(P＜0.01，n=8)；动作电位峰值由(68.49±2.33)mV下降至(54.71±0.81)mV(P＜0.01，n=8)。

Mosley Im glad youre my maker My Loyalty lies in your hands, youre my breath taker Your body, your kiss is in unknown demand So take command, go Timbo I be the same when it all goes up I be the same when it all goes down Not the first one, open it up I be the last one closin it out Dont know if Ill give you a shot yet Lil Mama Im peepin your style Do I think youre dope enough, yup One way of findin it out The way you came at me, boo Dont care, not afraid Im like Wild Really want it from head to toe Question if she gon let it out Anyway the hour glass go I dont worry anyhow Why dont we see where it go Lets figure it out When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after Dark Owww, Oooohhh Owww Come on SoShy I got a little secret for ya I never sleep when comes the night But everytime I smack my fingers I switch back into the light My moon belong to your sun Your fire is burning my mind Is it love or is it lust Something that I just cant describe Am I the one and only Cause youre the only one It felt so long and lonely Waiting for you to come Its lookin bright and early Im willing to close my eyes This is the unusual story Timbo and SoShy When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after Dark Heyy, Heyy, Heyy, Go Nelly!

莫斯利先生您好我很高兴youre进出口公司我的忠诚度掌握在你们手中，youre我的呼吸接受者你的身体，你的吻是在未知的需求因此，接受命令，去廷博俺是相同的时，所有的上涨俺是相同的时，一切都下降不是第一个，打开它俺是最后一次closin出来 Dont知道病患者给你一个镜头还进出口peepin律妈妈你的风格我认为youre涂料不够，烨一个方法是findin出来你的方式来我，嘘 Dont护理，不怕像野生进出口真的想从头到脚它问她是否坤把它放出但无论如何，沙漏去余请勿担心无论如何为什么dont我们看到它去可以让数字出来当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在上午的后当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在天黑后早晨 Owww，Oooohhh Owww 来吧SoShy 我得到了亚一个小秘密我从来不睡觉的时候来晚但是，每次我拍击我的手指我转回到光明我属于你的月亮太阳你的火是我心中燃烧是爱还是欲望一些我公正不能描述我是唯一的原因youre唯一的它认为这样漫长而孤独的等着你来它lookin一大早进出口愿意接近我的眼睛这是不寻常的故事廷博和SoShy 当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在上午的后当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在天黑后早晨日Heyy，日Heyy，日Heyy，直接耐莉！

ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果：hMSCs诱导前为纺锤形，诱导后第2天部分细胞即开始发生形变，呈球形或短棒状，1周后胞浆中颗粒增多，约20～30%细胞边缘呈毛刷样变化；hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义，CD90未诱导时表达呈弱阳性，诱导后明显增高(P.05)；ANP和CX43在诱导前无表达，诱导后第2周开始表达且表达随时间逐渐增强，但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加，低浓度诱导组低于标准浓度诱导组，组间差异有统计学意义(P.05)，G_0/G_1期细胞比例诱导后较对照组显著减少(P.05)；β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强，在第6周时均达到高峰，第8周时表达开始衰减，Bcl-2、Bax基因表达呈时间依赖性变化，hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

Research on Keeping Quality of Different Flesh Types of Prunus persica L.Niu Liang, Wang Zhiqiang, Liu Shu'e, Song YinhuaAbstract: Effects of post-harvest cold storage and Modified Atmosphere Package on fruit hardness, weight losing and soluble solid content of different peach or nectarine varieties were investigated. The results showed that:(1) There were obviously differences of fruit hardness among varieties of different flesh types. In room temperature, FH of '24-30' and 'Zhongtao No.2' went down slower than 'Zhongyoutao No.5' and 'Zhongyoutao No.10'. In cold storage, '24-30', FH of Zongyoutao No.5' and 'Zhongyoutao No.10' descent quickly after 2 weeks of storage while that of 'Shuguang' and 'Zhongtao No.2' quickly descent just after storage.(2) MA package could effectively slow down the decreasing of FH. There were obvious differences among different varieties while 'Zhongyoutao No.10' was less sensitive than others.(3) Soluble solid content went down after harvest and the procedure was slower in lower temperature. In room, MA package seems accelerate the descendent of SSC.

采用不同肉质类型的桃、油桃果实为材料，对采后低温和自发气调包装等对果实硬度、失重和SSC含量等的变化进行了研究，结果表明：(1)不同类型桃、油桃采后果实硬度变化差异较大，常温下24-30和中桃2号果实硬度下降较慢，而中油桃5号、10号较快，在冷库低温条件下，24-30、中油桃5号和中油桃10号两周后硬度出现迅速下降，而曙光和中桃2号在贮藏后即迅速下降，带皮硬度与去皮硬度之间有一定差异，但变化趋势基本一致；(2)MA包装能有效地延缓采后桃、油桃果实硬度的降低，在常温及低温条件下均是如此，不同肉质类型间差异明显，曙光和中桃2号非常敏感，而中油桃10号则不太敏感，即MA包装对其果实硬度降低的延缓没有其它品种明显；(3)采后桃、油桃果实的SSC含量均有一定的降低，低温下该过程较慢，MA包装在常温下有加剧SSC下降的趋势，低温下则不明显，这可能与常温下采后立即进行MA包装不利于&田间热&及呼吸热的及时散发而加剧呼吸有关。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。