在中心

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

In 1997, the department cracked 50 major trafficking cases, and neutralised four heroin attenuating, 12 packing and two storage centres.

在一九九七年内，香港海关侦破了50宗主要的贩毒案件，瓦解了4个海洛英稀释中心、12个包装中心和两个藏毒地方。

The dynamic constraints on the system are second-order nonholonomic constraints. It is assumed that the rigid bodies are hinged at the center of percussion. By the translational acceleration inputs at one of the rigid bodies, the system has two different kinds of rotation around the center of percussion.

该系统的动力学约束是二阶非完整的，通过给定其中一个刚体的加速度输入，并假设被动关节安装在刚体撞击中心处，系统可以产生两种不同形式的绕撞击中心的转动。

Sue is a founding Director, former President and current Secretary/Treasurer of The Technology Parks Australian and New Zealand, a former Regional Chair of the Australian Institute of Management past Director, National Treasurer of The Australian and New Zealand Association of Business Incubators, and a former member of the Logan TAFE Better Business Advisory Committee. Sue was also a Board member of Ballarat Regional Adult and Community Education for two years.

苏·贝尔在巴拉瑞特大学担任重要管理职位，管理着极为成功的Greenhill 企业中心、布里斯班科技园及布里斯班的Logan信息技术中心，她还是澳大利亚和新西兰科技园前主席，澳洲管理协会前区域主席，澳大利亚和新西兰企业孵化器协会前主任、国家会计师，以及Logan TAFE更优商业咨询委员会(Logan TAFE Better Business Advisory Committee)前会员，同时还担任巴拉瑞特区域成人与社区教育董事会成员两年。

This is a time to "gather as one," at what many consider the gateway for the American Church, the heart of the heart of the Bible Belt: Nashville.

这是「出来如同一人」的时刻，就在许多人认为是美国教会城门，是圣经区域带中心的中心地点：纳什维尔。

In the upper atmosphere, ionosphere, magnetosphere. At present, there are a variety of advanced ground based instruments, such as proton magnetometer, fluxgate magnetometer, Digisonde, GPS ionospheric TEC and scintillation monitor, HF Doppler, all-sky meteor radar and airglow imager etc. The data monitoring and processing center can monitor the observation instrument working state in all stations and collect and process data through Internet. Some of processing data results can be published on the web near real time( http://space.iggcas.ac.cn ).

目前，台链拥有质子磁力仪，磁通门磁力仪，电离层数字测高仪， GPS TEC 和电离层闪烁监测仪，高频多普勒探测仪，全天空流星雷达和气辉成像观测仪等多种先进的地基空间环境综合观测设备，台链监控和数据中心对各台站观测仪器和数据状态进行监控，观测数据实时传输到台链数据中心进行处理和保存，并在互联网上实时发布（网址： http：//space.iggcas.ac.cn ）。

After transverse Zeeman effect is introduced to reduce the mode coupling, the laser could produce two orthogonal linear polarized components with 5MHz beat frequency. The effect of the magnetic field's direction to generation of the dualfrequency and the polarization property of the laser is investigated Based on theoretical study and experimental investigation to the beat frequency tuning character, a novel method for stabilizing the laser beat frequency is brought forward.

现代机械工业的发展使得机床、三坐标测量机等的运行速度已提高至1m/s，为适应这种发展趋势，本文研究了高频差（5MHz）激光干涉仪的光源、稳频和外差信号的处理等基本问题，建立了测量速度可达1m/s的高测速激光干涉仪，并对其在加工中心主轴热漂移测量系统和加工中心误差实时测量系统中的应用进行了研究。

And because of the high demand for manpower, representatives from Cyber City Teleservices Ltd, the largest call center in the country, is looking to as far as Zamboanga City to recruit call center agents to fill up the many available positions the company offers.

并且由于高需求的人力，代表数码城市电信有限公司，最大的呼叫中心在该国正在寻求尽可能三宝颜市招聘呼叫中心人员，以填补现有的许多公司提供的职位。

Members of the indigenous group began taking courses in January 2002 at the Cultural and Life Skills Centre in Wagga Wagga, established in part by the project last November.

今年1月，原住民们开始在瓦嘎瓦嘎的文化生活技能中心上课，该中心即是得力於此计划才於去年11月竣工。

Built between the 11th and 18th centuries, the Old Town, the Lesser Town and the New Town – with their magnificent monuments, such as Hradcani Castle, St Vitus Cathedral, Charles Bridge and numerous churches and palaces, built mostly in the 14th century under the Holy Roman Emperor, Charles IV – speak of the great architectural and cultural influence enjoyed by this city since the Middle Ages.

布拉格历史中心建于11 至18 世纪之间，老城、外城和新城主要是在14世纪时神圣罗马帝国查理四世统治时期建造的。中心拥有诸如荷拉德卡尼城堡、圣比图斯大教堂、查理桥以及数不胜数的教堂和宫殿等绚丽壮观的遗迹。自中世纪起它就以其建筑和文化上的巨大影响而著称于世。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。