在上游

Look now, suffer financial storm to affect bigger is upriver manufacturing industry enterprise and it is oriented enterprise in order to export, because kept away from these two kinds of enterprises, in investment, chose to satisfy Chinese mainland to consume the enterprise of demand, fund of China of Chinese larch capital is in epicycle banking crisis, just escaped one disaster.

现在看来，受金融风暴影响较大的是上游制造业企业和以出口为导向的企业，正是由于避开了这两类企业，在投资中，选择了满足中国本土消费需求的企业，红杉资本中国基金在本轮金融危机中，才躲过了一劫。

At that day, beside the Ganges, I just saw the death.

就在那天，在尼泊尔恒河上游，我看到了死亡。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

Sacagawea and her husband lived among the Hidatsa and Mandan Indians in the upper Missouri River area (present-day North Dakota).

萨卡加维亚跟丈夫生活在密苏里河上游地区，他们与希多特萨和曼丹印第安人生活在一起。

The influences of horizontal merger on upper suppliers and the profit of the whole supply chain were investigated,and the effects of the power structure within a supply chain during horizontal merger were analyzed.

研究了在Cournot市场竞争条件下，生产企业的横向并购对于上游供应商和整条供应链利润的影响，以及供应链权力配置在并购中的效应。

Many farmers in the upper reaches of the Klamath Basin are replacing such wheel-line irrigation with more modern methods, and improving the water efficiency of their operations.

许多在克拉马斯盆地上游的农民以更现代化的方式来取代这种轮线灌浇方式，从而改善在操作中的用水效率。

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通过对EIAV分离株WH17的LTR与L株、DA和DLA株的LTR序列进行比较，发现其在在U3-R结合处多一个E-box基序，推测该基序的变化可能会起到促进转录作用，为此将EIAV强毒株(DLA-25)、驴白细胞弱毒株(DLA-118)、EIAV分离株WH17以及U3-R结合处的E-box基序点突变的LTR分别克隆到pCAT3-basic质粒中的报告基因氯霉素乙酰转移酶的上游，获得一系列受不同LTR控制的CAT表达质粒。

In the present study, the unidirectional CaMV 35S promoter has been modified to a bi-directional promoter by fusing its minimal promoter element to the 5′end of CaMV 35S promoter in the opposite orientation.

在本研究中，通过在CaMV35S启动子的上游反向连接其小启动子，将其改造为双向启动子。

Analysis of nuclear extracts of human cells has indicated a similar excision repair mechanism for nick-directed mismatch correction in higher cells, and several reconstituted systems that rely on purified human proteins have been described, such as hMutSα、hMutSβ、hMutLα. Recently, scientists discovered that nickel could be inhibitors of DNA repair. Nickel can greatly enhance the mutagenicity and genotoxicity of compounds in cigarette by inhibiting the nucleotide excision repair pathway in human cells. We have confirmed that nickel could inhibit MMR of E. coli and human and presumed that nickel inhibited repair before MutH incision of the MMR in E. coli. However, there is no evidence of mechanism of nickel inhibiting MMR in human cells.

镍是存在於香菸中的微量金属，近年来有学者发现镍是DNA修复反应的抑制剂，长期抽烟的人会容易因为香菸中化合物攻击DNA而引起突变，进而引起肺癌，而镍会抑制核苷酸移除修复系统（nucleotide excision repair；NER），所以使得体内的DNA损伤无法修复；过去实验室已经证实大肠杆菌的核酸配对错误修复系统会受到镍等重金属的抑制，并且推测出镍的抑制反应是抑制在修复反应的上游nicking的步骤；另外，也证实了人类的核酸配对错误修复系统也会受到镍的抑制，然而还没研究证实会抑制在核酸配对错误修复反应的哪个步骤。

AT POPE'S CREEK, on America's Potomac river, there's a pre-Columbian rubbish tip of oyster shells covering 30 acres, to an average depth of ten feet.

在波拖马可河上游，有一条POPE'S小溪。在哥伦布发现美洲大陆之前那里就有一处面积达30英亩，平均深10英尺的牡蛎壳的垃圾堆。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。