在上游

2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点（44、45、49和50号内含子以及5'端启动子区的序列）设计引物，在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾：CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身，经高分辨率的变性聚丙烯酰胺凝胶电泳后，利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置，进行扫描分析，确定产物片段的大小。

It can provide scientific basis for establishing healthy ecological environment and formulating policies of environmental management.

在综合分析长江上游四川省生态环境特点的基础上，阐明了本区的生态地位及存在的主要生态问题。

A gate or ball type valve can be used or the isolation valve can be installed further upstream.

可以采用闸阀或球阀，或者可以将隔离阀安装在更上游的地方。

China has three operational dams on the river's headwaters in Yunnan and two more are being built.

中国在云南上游流域有三座正在运行的水坝，目前在建的还有两座。

Similar sediment pollution scenarios are occurring in the headwaters of the Salween and Yangtze Rivers.

在萨尔温江和长江上游也存在类似的泥沙污染状况。

As a scientist who has worked on this topic for some years, it is my judgment that 80 to 95 percent of the direct sediment contributions into the headwaters of these rivers are now attributable to road erosion and landslides.

作为一名曾在该领域工作多年的科学家，我判定，这些水系上游的直接泥沙排放量中，有80%到95%来自于道路侵蚀和滑坡。

In fact, in the hoggery in Ding Lei not only integrated of upper reaches grow line of business, and high grade pork has similar France wine possibly also through development manorial in that way sightseeing value.

事实上，在丁磊的养猪场里不但整合了上游的种植业，而且优质猪肉通过开发也可能拥有类似法国葡萄酒庄园那样的观光价值。

In our model of horizontal competition between an informed and an uninformed firm with a common upstream supplier, material and information flows intersect through leakage of demand information to unintended recipients.

在我们的同业竞争（包括拥有共同的上游供应商的知情和不知情的公司）的模型中，物料流和信息流通过需求信息泄露给看似无意的人而相互交织。

The study probe into at when extreme rainfall happenning incident research purpose department on city, estimate its flow of flood crest to defend and provide disaster relief the reference carried on the exercise of in follow-up ; While estimating the the depth of extreme rainfall , use separately: World Meteological Organization statistic method, storm transposition and dew point adjustment method and average recurrence interval rainfall depth factor method, inquire into and act as river valley wutu the upper reaches of Keelung and collect in the water area separately, wutu station with flood-diversion stands in the Huo-Shao-Liao station before Yuan-Shan-Tzu flood-diversion, the depth when the extreme rainfall happens separately, step forward and mix the set of design hyetograph by extreme rainfall ,finally,mix geomorphologic instantaneous unit hydrograph afterwards, in order to estimate the flow of flood crest produced cause by extreme rainfall incident .

本文之研究目的系探讨当极端暴雨事件发生於都会区时，推估其洪峰流量以作为后续防救灾演练之参考。在推估极端暴雨之降雨深度时，分别采用：世界气象组织统计法、暴雨移置与露点调整法及平均重现期距雨量因子法，分别推求当基隆河流域五堵上游集水区，员山子分洪前之火烧寮站与分洪后之五堵站，分别发生极端暴雨时之雨量，进一步将极端暴雨量套配设计雨型，最后再搭配地貌型瞬时单位历线模式，以推估因发生极端暴雨事件而产生之洪峰流量。

On the basis of summarizing the calculating methods of minimum instreaming ecological flow at home and abroad, the improved wetted perimeter method is adopted to determine minimum instreaming ecological flow of upper reaches of Hangjiang River.

在探讨总结国内外计算河道内最小生态流量(minimum instream ecological flow)方法的基础上，针对汉江流域的实际情况，选用改进湿周法对汉江上游石泉、安康和白河3个水文断面的最小生态流量进行定量估算。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。