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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.
结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在囊胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。
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But beginning in the attempt, not to establish, but to evade —— not to discover a principle, but to escape a postulate —— not to settle the nature of right and wrong, but to determine what was not wrong of a particular nature,—— Casuistry went on with its dexterous refinements till it ended in so attenuating the moral features of actions, and so belying the moral instincts of Our being, that at length the conscience of mankind rose suddenly in revolt against it, and consigned to one common ruin the system and its doctors.
但在其开始的企图中,它不是在建立而是在规避,——不是在发现一条原则而是在逃避一个假定——不是在确定是和非的性质而是在行为的决定在一个特殊性质中哪些是不错的,——诡辩学就是这样用了它的巧妙的高论继续发展下去,直到它最后过分地削弱行为的道德特征,过分地诽谤了我们人类的道德本能,以致最后人类的良心突然起来反抗它,并把这体系和其博士们埋葬在一个共同的废墟中。
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There was one intriguing prospect brought to the purple and gold this summer, however, as2007 draftee Sun Yue decided to make his way over to the US and begin his NBA career.
这个夏天还有个令人期待的球员加入这紫金之师,在07年被选中的孙悦决定在美国开始他的NBA生涯。
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Just before the mass began hundreds of doves were released at the end of a two-hour Concert of Hope that enlisted the participation of such major entertainment figures as tenor Ronan Tynan, Jose Feliciano, opera star Marcello Giord and Harry Connick Junior.
在弥撒开始不久将会有上百的各自在两个小时的希望音乐会尾声被放生,这个音乐会主要的参加者包括男高音歌唱家RT,JF,歌剧明星MG和HCJ。
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Formosus in Cambodia begins towards the end of the dry season (March-April) and is approximately 3 months in duration.
在繁殖季节的红龙鱼在柬埔寨开始接近年底的旱季( 3-4 月)和大约3个月的时间。
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Isa began putting her phone number on assignments, and when she came by there was a certain forwardness about her, a kind of "can you-handle-this?"
ISA开始放她的电话在任务报告上,并且当她在的时候,有一种冲劲,一种"你能搞定"吗的态度。
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And let us now address in another study that is starting now, the Futura study, which is called Oasis 8, which will compare two doses of unfractionated heparin that will be given in addition to fondaparinux in patients who will undergo PCI for acute coronary syndrome.
另外,在刚刚开始的Oasis 8试验中,研究人员将比较将进行PCI治疗的两组病人在磺达肝癸钠外加不同普通肝素剂量的效果。
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Q: You mentioned the graining of the tyres in the second stint; did you have it also at the start of the third?
问:你提到了在第二阶段轮胎的粒化,你在第三阶段的开始也有相同的情况吗?
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I like to say that the UP is serial in the large and iterative in the small.
在一个 UP 项目的开始,你关注的是在 Inception 阶段的项目初始活动。
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No, eventually, but there will be, at the beginning of recovery, there will be jobless.
在最后是不会有这种情况的,但是在经济复苏的开始阶段是会出现的,出现失业。
- 推荐网络例句
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I can not make it blossom and suits me
我不能让树为我开花
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When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.
当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。
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The lawyer's case blew up because he had no proof.
律师的辩护失败,因为他没有证据。