在...时候

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Rock Around The Clock One, two, three o'clock, four o'clock, rock Five, six, seven o'clock, eight o'clock, rock Nine, ten, eleven o'clock, twelve o'clock, rock We're gonna rock around the clock tonight Put your glad rags on and join me, hon We'll have some fun when the clock strikes one We're gonna rock around the clock tonight We're gonna rock, rock, rock,'til broad daylight We're gonna rock, gonna rock, around the clock tonight When the clock strikes two, three and four If the band slows down we'll yell for more We're gonna rock around the clock tonight We're gonna rock, rock, rock,'til broad daylight We're gonna rock, gonna rock, around the clock tonight When the chimes ring five, six and seven We'll be right in seventh heaven We're gonna rock around the clock tonight We're gonna rock, rock, rock,'til broad daylight We're gonna rock, gonna rock, around the clock tonight When it's eight, nine, ten, eleven too I'll be goin' strong and so will you We're gonna rock around the clock tonight We're gonna rock, rock, rock,'til broad daylight We're gonna rock, gonna rock, around the clock tonight When the clock strikes twelve, we'll cool off then Start a rockin' round the clock again We're gonna rock around the clock tonight We're gonna rock, rock, rock,'til broad daylight We're gonna rock, gonna rock, around the clock tonight

罗克 Around 钟一，二，三点钟，四点钟，岩石五，六，七点钟，八点钟，岩石九，十，十一点钟，十二点钟，岩石我们正去向昼夜不停今晚的岩石穿上你的最好的衣服和加入我，亲爱的我们将获得乐趣中的一些当钟用击打我们正去今晚昼夜不停摇动的的时候，我们正去岩石，岩石，岩石，鼻音化符号大白天我们正去向去昼夜不停今晚的岩石的岩石当钟响了二，三和四下，如果乐队让生活松弛些的时候，我们将为我们正去今晚昼夜不停摇动的更多对叫嚷我们是去岩石，岩石，岩石，鼻音化符号大白天我们正去去的岩石向昼夜不停今晚的岩石当排钟正确在第七天堂中按五，我们将是的六和七叫的时候，我们正去向昼夜不停今晚的岩石我们正去岩石，岩石，岩石，鼻音化符号大白天我们正去向去昼夜不停今晚的岩石的岩石当它也是八，九，十，十一的时候，我将强有力地是 goin 和那样你希望我们是去昼夜不停今晚的岩石我们正去岩石，岩石，岩石，鼻音化符号大白天我们正去向去昼夜不停今晚的岩石的岩石当钟给予十二我们将使冷下来的时候，那时再次以开始一 rockin' 昼夜不停我们是去昼夜不停今晚的岩石我们正去岩石，岩石，岩石'，鼻音化符号大白天我们正去向去昼夜不停今晚的岩石的岩石

00Any man of mine better be proud of me [00:23.00]Even when I'm ugly he still better love me [00:26.00]And I can be late for a date that's fine [00:29.00]But he better be on time [00:32.00]Any man of mine'll say it fits just right [00:35.00]When last year's dress is just a little too tight [00:38.00]And anything I do or say better be okay [00:41.00]When I have a bad hair day [00:46.00]And if I change my mind [00:49.00]A million times [00:52.00]I wanna hear him say [00:54.00]Yeah, yeah, yeah, yeah, yeah I like it that way [01:01.00]Any man of mine better walk the line [01:06.00]Better show me a teasin' squeezin' pleasin' kinda time [01:13.00]I need a man who knows, how the story goes [01:18.00]He's gotta be a heartbeatin' fine treatin' Breathtakin' earthquakin' kind [01:26.00]Any man of mine [01:33.00]Well any man of mine better disagree [01:36.00]When I say another woman's lookin' better than me [01:40.00]And when I cook him dinner and I burn it black [01:43.00]He better say, mmmm, I like it like that yeah [01:47.00]And if I changed my mind [01:50.00]A million times [01:53.00]I wanna hear him say [01:55.00]Yeah, yeah, yeah, yeah, yeah I like it that way [02:01.00]Any man of mine better walk the line [02:08.00]Better show me a teasin' squeezin' pleasin' kinda time [02:14.00]I need a man who knows, how the story goes [02:20.00]He's gotta be a heartbeatin' fine treatin'Breathtakin' earthquakin' kind [02:28.00]Any man of mine [02:37.00]Let me hear you say yeah, yeah, yeah, yeah, yeah I like it that way [02:44.00]Any man of mine better walk the line [02:54.00]Better show me a teasin' squeezin' pleasin' kinda time [03:00.00]I need a man who knows, how the story goes [03:06.00]He's gotta be a heartbeatin' fine treatin'Breathtakin' earthquakin' kind [03:15.00]Any man of mine [03:22.00]You gotta shimmy shake [03:24.00]Make the earth quake [03:25.00]Kick, turn, stomp, stomp, then you jump [03:30.00]Heel to toe, Do Si Do [03:32.00]'Til your boots wanna break [03:34.00]Til your feet and your back ache [03:36.00]Keep it movin''til you just can't take anymore [03:39.00]Come on everybody on the floor [03:44.00]A-one two, a-three four [03:47.00]Hup two, hum [03:55.00]This is what a woman wants...

比较好的任何我的男人 [00：20.00] 以我为傲 [00：23.00] 即使当我是丑陋的他仍然比较好爱我 [00：26.00] 和我能去一个罚款的日期迟到 [00：29.00] 但是他最好准时任何的 [00：32.00] mine'll 的男人说它仅仅正确适合 [00：35.00] 当去年的洋装仅仅有点儿是紧的时候 [00：38.00] 和任何事我做或说更多是好 [00：41.00] 当我心情不好的时候 [00：46.00] 而且如果我改变我的想法 [00：49.00] 一百万乘 [00：52.00] 我想要听到他说 [00：54.00] 是的，是，是的，是，是的我那样喜欢它任何我的比较好的散步男人线的 [01：01.00] [01：06.00] 最好给我看 teasin'squeezin' pleasin'有一点时间 [01：13.00] 我需要一个知道的男人，故事去的方式 [01：18.00] 他是必须是 heartbeatin'罚款 treatin'Breathtakin' earthquakin'类型任何我的男人 [01：26.00] [01：33.00] 涌出任何我的男人比较好的不同意 [01：36.00] 当我说另外的一个女人的 lookin 的时候'比较好的超过我 [01：40.00] 和当我烹调他晚餐，而且我燃烧它的时候黑色的 [01：43.00] 他比较好的发言权， mmmm，我像那一样喜欢它是的 [01：47.00] 而且如果我改变了我的想法 [01：50.00] 一百万乘 [01：53.00] 我想要听到他说 [01：55.00] 是的，是，是的，是，是的我那样喜欢它任何我的比较好的散步男人线的 [02：01.00] [02：08.00] 最好给我看 teasin'squeezin' pleasin'有一点时间 [02：14.00] 我需要一个知道的男人，故事去的方式 [02：20.00] 他是必须是 heartbeatin'罚款 treatin'Breathtakin'earthquakin'类型任何我的男人 [02：28.00] [02：37.00] 让我听到你说是的，是，是的，是，是的我那样喜欢它任何我的比较好的散步男人线的 [02：44.00] [02：54.00] 最好给我看 teasin'squeezin' pleasin'有一点时间 [03：00.00] 我需要一个知道的男人，故事去的方式 [03：06.00] 他是必须是 heartbeatin'罚款 treatin'Breathtakin'earthquakin'类型任何我的男人 [03：15.00] [03：22.00] 你车轮摆动必须摇动吗 [03：24.00] 制造地球地震 [03：25.00] 踢，旋转，跺脚，跺脚，然后你跳跃 [03：30.00] 尾随以趾踏触， Si 做吗 [03：32.00]'胡麻你的长靴想要打破 [03：34.00] 胡麻你的脚和你的后面痛 [03：36.00] 保存它 movin''胡麻你就是能不再轮流 [03：39.00] 在地板上受到在每个人身上的影响 [03：44.00] 一-三，一-七 [03：47.00] Hup 二，嗡嗡声 [03：55.00] 这是一个女人想要的。。。

When he heard the sound of gunfire then get down to 20 gallons of liquor, a stove spade, a Huoqian, two Tonghu, Tongguo and Grill when the shuttle is in Cluj read "Bible" when , Seems to be the wizard finish spell want the same thing as a coincidence of magic, here is novel in this second stage of reference to "Bible", here is Kru shuttle by a gift of God, when the third novel to And "Bible", in Cluj shuttle by the threat of terrorism and disease, the precarious life of the time, it seems that the author of a demon Satan for a guest, is the Kru people of the shuttle Buxiao the "original sin" Another penalty, or is he received the last of God's gift to God and not the consequences of Thanksgiving, when he is determined to be more devout starting point, this echoes the "Bible" in the words "in trouble when you call for me I will Laijiu you, and you have to praise me,"Mingmingzhizhong Defoe who arranged a deal with the integrity of God, but Kru shuttle in this transaction in the integrity of performance is not very good, so in God Pre-paid to the equivalent of his wealth, its very credibility of not giving a penalty.

当他听到炮声随后顺着枪声得到二十加仑地酒、一把火炉铲、一把火钳、两个铜壶、铜锅和烤架的时候，正是克鲁梭在读《圣经》的时候，好像精灵念完咒语得到想得到的东西一样巧合一样具有魔力，这里是小说在这一阶段第二次提及《圣经》，这里便是克鲁梭得到的上帝的馈赠，当小说第三次提及《圣经》时，是在克鲁梭受到恐怖和疾病的威胁，性命岌岌可危的时候，好像是作者安排的一场恶魔撒旦的一次客串，是克鲁梭为人子而不孝的"原罪"的又一次惩罚，抑或是他得到上次上帝的馈赠而对上帝感恩不够的后果，此时也是他下决心会更加虔诚的起点，这便呼应了《圣经》里的话"在你患难时呼唤我，我就会来救你，而你要赞美我"，冥冥之中笛福安排了一次人与上帝的诚信交易，但是克鲁梭在这次交易中表现的诚信不是很充分，所以在上帝预先付给他那些等价财富后，对其的不很诚信给予了惩罚。

I hereby declare, on oath, that I absolutely and entirely renounce and abjure all allegiance and fidelity to any foreign prince, potentate, state, or sovereignty of whom or which I have heretofore been a subject or citizen; that I will support and defend the Constitution and laws of the United States of America against all enemies, foreign and domestic; that I will bear true faith and allegiance to the same; that I will bear arms on behalf of the United States when required by law; that I will perform noncombatant service in the Armed Forces of the United States when required by the law; that I will perform work of national importance under civilian direction when required by the law; and that I take this obligation freely without any mental reservation or purpose of evasion; so help me God.

宣誓人在此发誓宣告：我绝对地、完全地公开断绝、发誓放弃对我目前是臣民或公民的任何美国以外的王室、当权者、国家或任何主权的所有效忠和忠诚；面对所有的敌人，不论是外国的还是国内的，我将支持和保卫美国的宪法和法律；我将对美国带着真诚的信念和忠贞；我将在法律要求的的时候为美国拿起武器；我将在法律要求的时候为美国军队从事非战斗性的服务；我将在法律要求的时候在文官的领导下担当国家的重任；而且我将自由地履行我的义务和职责，而不带任何思想的保留和故意逃避；上帝啊，帮助我吧。

For now, we should sit back and enjoy the show; watch the man start to look like Snoop Dogg the longer his hair gets, and try to remember a time when the mention of CB4 made you think of this before thinking of a guy who can rack on you from the block or win a game on a spot-up J with the clock winding down.

现在，我们应该做的是坐好准备看波什的精彩表演吧；等著看著这个一头长发长的像Snoop Gogg（美国著名Hip-Hop歌星）的男人，当什麼时候一提起CB4这个名字时候会令你想起这个可以在你面前送出热辣辣的火锅或者是在比赛时间所剩无几的时候投中一个比赛的制胜球，那时候他已经成为了炙手可热的巨星。

I dance around this empty house Tear us down Throw you out Screaming down the halls Spinning all around and now we fall Pictures framing up the past Your taunting smirk behind the glass This museum full of ash Once a tickle Now a rash This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down 9, 8, 7, 6 5 4, 3, 2, 1, fun Echoes knocking on locked doors All the laughter from before I'd rather live out on the street Than in this haunted memory I've called the movers Called the maids We'll try to exorcise this place Drag my mattress to the yard Crumble tumble house of cards This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down 9, 8, 7, 6 5 4, 3, 2, 1, fun Oh, I'm crawling through the darkest home My key don't fit my life no more I'll change the drapes I'll break the plates I'll find a new place Burn this fucker down do do do do dodo do do do do do dodo do do do do do dodo do do do do do dadadada do do do do dodo do (9, 8, 7, 6 5 4, 3, 2, 1) do do do do dodo do do do do do dodo do do do do do dodo doo This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down

本人舞蹈解决这个空房子撕裂我们失望你驱逐出去尖叫了大厅纺纱各地，现在我们属于图片框过去您的嘲弄微笑背后的玻璃该博物馆完全灰一旦痒现在皮疹这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来 9 ， 8 ， 7 ， 6 5 4 ， 3 ， 2 ， 1 ，乐趣回声敲锁车门所有的笑声之前我宁愿住在街道上进行比这个闹鬼记忆我已经称为推动者所谓的女佣我们会尝试傩这个地方拖曳我床垫的码崩溃暴跌众议院卡这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来 9 ， 8 ， 7 ， 6 5 4 ， 3 ， 2 ， 1 ，乐趣噢，我最黑暗的爬行通过主页我的钥匙不适合我的生活没有更多我会改变窗帘我要打破盘子我会找到一个新的地方烧伤这个笨蛋下跌做这样做这样做渡渡鸟做这样做这样做渡渡鸟做这样做这样做渡渡鸟这样做这样做dadadada 这样做这样做渡渡鸟做（ 9 ， 8 ， 7 ， 6 5 4 ， 3 ， 2 ， 1 ）做这样做这样做渡渡鸟做这样做这样做渡渡鸟这样做这样做渡渡鸟斗这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来

Listening can be divied into three levels, which are charcterized by certain behaviors that affect listening effciency .most often ,people have difficulty listening effectively when in a conflict situation , when dealing with emotional people, when having criticism directed at them, when being disciplined or when feeling anxious , fearful or angry.the following descriptions of the three levels will help you understand the distinction between how each level is expressed?

倾听可以分为三个层次，而不同的层次是以能够影响听力效率的某种特定行为为特征和表现的。通常，当人们遇到下列几种情况时，有效率的倾听则显得困难，例如当人们和其他人产生冲突或争执的时候，和易激动的人相处的时候，被他人批评的时候，被处罚的时候，感到焦虑、恐惧和愤怒的时候。以下对这三个层次的描述有助于你理解三者在表现上的区别。

When I originally recorded this section of "A Change of Seasons," I used simpler voicings in a few places. For the purpose of this study, however, I made them a bit more interesting. For example, on the record I played a simple Em chord in bar 9, but here I decided to use Em(add9) instead. Both work well, but I feel Em(add9) is more dramatic. On the original "A Change Of Seasons," I repeated the Asus2 chord in bar 6 that I played in bar 2. Here, I substituted it with an open-string Amaj9 voicing.

但我最初在录A CHANGE OF SEASONS中的这个部分的时候，我在几个地方用了更简单的声音，而这种研究的意图，无论如何，我让它们变得更加有意思，举个例子，在录音的时候，我在9品的地方弹一个简单的EM和弦，但最后我又决定用一个EM（ADD9）和弦来取代，虽然效果都行，但是我觉得EM（ADD9）更加有色彩，在原来的A CHANGE OF SEASONS里面，我在弹第二节的时候重复了两遍第6品的ASUS2和弦，现在，我却用一个开放按法的Amaj9和弦取代了它。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。