在...时候

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Only here and now for the first time in his life Pierre fully appreciated the enjoyment of eating when he was hungry, of drinking when he was thirsty, of sleep when he was sleepy, of warmth when he was cold, of talking to a fellow creature when he wanted to talk and to hear men's voices.

只有在这里，只有在这种时刻，只有当他饥饿的时候，皮埃尔才第一次完全体会到吃东西的快乐，只有当他口干的时候，才体会到喝水的快乐，只有当他寒冷的时候，才体会到温暖的快乐，只有当他想睡觉的时候，才体会到进入梦乡的快乐，只有当他渴望和人谈话和听见人的声音的时候，才体会到和人谈话的快乐。

For the sake of our se indocile kidses, be sometimes connect oneself think of, oneself of do of but all don't think, just just is really not easy for the sake of the other people, I really feel good regret, the good heart be vexed, I when then can have 1:00 use, my in the mind really panic, sometimes want oneself really ridiculous, but sometimes is very strong and unyielding, I since the childhood have no oneself of heaven, I really don't know my later of day is what appearance, if when I remind of these matter of time, my tears with through continuously turn to move in the tears frame, I although have no too Gao of culture, my whole life in the middle I with lose two opportunity, I don't want to be losing, but when I want to do point what of time, my heart was really full of hope, but did some energy all have no, I very timidity, there is no strength yes, I don't know how to do, if the life really want me to have no position in the world here, why still make me on the hoof, I am exactly and when my life in the middle, greatest beginning

就是为了我们这些不听话的孩子，有时候连自己想的，自己的做的却都不想了，就只是为了别人而已，真是不容易啊，我真是感到好懊悔，好心烦，我什么时候才能有一点用啊，我的心里真的发慌啊，有时候想想自己真的很可笑啊，但有时候却有很坚强不屈，我从小就没有自己的天堂，我真的不知道我的以后的日子是什么样子的，如果当我想起这些事的时候，我的泪水就以经在泪框里不停的转动，我虽然没有太高的文化，但是我一生当中我以失去了两次机会了，我不想在失去了，可是当我想做点什么的时候，我的内心充满了希望，可是真正的做起来一点力气都没有，我很胆小，没有力量是的，我不知道该怎么办，如果人生真的要我在这个世界上没有地位，为什么还让我活着，我到底什么时候才是我人生当中，最伟大的开始

Hesomagari na kotoba mo, kimi to no HITOKAKERA nanda yo nakushitari wa shinai aitakute, aitakute, KOKORO kaze ni tokashi nagara itsudatte, itsudatte, kimi no soba ni boku wa iru aitakute, aitakute, furedashite shimau omoi abushisugiru kimi no yokogao aitakute, aitakute, hoshi no kazu no yoru wo koete itsudatte, itsudatte, kimi no soba ni boku wa iru itsuka kitto, itsuka kitto, onaji yume ni nemureru youni boku mo kimi no HIKARI ni naru ABC, mienai mirai mo Can't you see?

你说再见？偏执的话语我一点儿也不会对你说不想失去你好想见面好想见面心都融化在了风中的同时什么时候什么时候我陪在你的身旁好想见面好想见面这份感情已经溢满你的侧脸美得炫目好想见面好想见面数着星星过了一夜什么时候什么时候我陪在你的身旁总有一刻总有一刻希望我们拥有同样的梦我也能成为你的光 ABC 看不到的未来 Can't you see？

That as from the beginning, a projection OF that form that assumed its position or condition in the earth as from the beginning, and with those so endowed with that as may be called an insight into psychic sources there may be visioned about a body its astral, rather its THOUGHT body, as is projected FROM same in such a state; especially so when there is the induction, or the inducing of, an unconsciousness of the normal brain, or normal mental body.

从开始的时候起，从开始的时候起假定它在地球上的位置或情形的形态的投射，由于赋予了可以被称作的内在精神本源，在本源里身体可以被想象成星形的，确切说就是思想体，同样可以以如此的一个状态来进行投射；特别在感应的时候，或一个正常的无意识大脑或正常的精神身体的进行意识诱导的时候。

Wang Hang: I am helping a lot of people, a lot of friends say you with me this website is very useful, when does the person need to help most actually, when he falls ill, when he is the flimsiest, at that time if you this website popularized valuable information to help them, I feel they can be written down all one's life certainly, and they can tell their friend, main discharge and growth, likelihood 70%, 80% it is to come from the conduct propaganda at public praise.

王航：我在帮助很多人，很多朋友跟我说你这个网站很有用，其实人什么时候最需要帮助，在他生病的时候，在他最脆弱的时候，那个时候假如你这个网站推广了有价值的信息帮助了他们，我觉得他们一定会记一辈子的，并且他们会告诉自己的朋友，主要的流量和增长，可能70%、80%都是来自于口碑的宣传。

Babyface--Simple Days I was born on the northside of a midwestern town There was nine of us living in a three bedroom house Didn't have much money, but we made do 'Cause Momma worked all day long And Daddy did too Did all they could Made sure we had clothes and food Went to public schools and we followed public rules We used second hand books but oh I didn't mind the books Momma taught us early in life Everybody's gotta struggle sometime Bring back those simple times of yesterday When a man was a man And a friend was a friend Bring back those simple times of yesterday When you said what you meant And you meant what you said Had a friend named Bobby Who always kept his word We went through schoolyard fights Had a thing for pretty girls Didn't cost much money To see the picture show So we hopped the bus into town That's where all the girlies used to go Fifty cents would buy us popcorn for two 'Cause Booby's girl she worked there And sometimes she'd break the rules Picnics in the summer Wouldn't wake up 'till noon And even though Bobby's gone now I can still hear him laugh In the middle of June Sometimes I sit, I wonder I reminisce I think of all the good times That we had in our lives Sometimes I can't get over How wonderful those days were Back in our lies Simple days for simple times

娃娃脸 -简单的天我是出生在northside一个中西部城市有9对我们生活在一个三间卧室的住房没有太多的钱，但我们作出这样做事业momma工作一天和爸爸没有太当时，所有他们可以确保我们有衣服和食品到公立学校和我们遵循公共规则我们使用的二手书籍但是，哦，我并没有考虑到图书 momma告诉我们，早在生活每个人的宝贝，有时斗争带回这些简单的时候，昨天当一名男子被一名男子和一个朋友是朋友带回这些简单的时候，昨天当你说你的意思和你的意思是什么，你说有一个朋友，命名为鲍比谁始终保持他的字我们经历了校园打架了的事，漂亮的女孩没有成本多少钱看到图片显示因此，我们跳频巴士纳入城市这是所有girlies使用去 50美分，将购买我们的爆米花为2 事业诱杀的女孩，她在那里工作有时，她要打破规则旅行，在今年夏天不会唤醒'，直至中午十二时即使鲍比的经历，现在我仍然可以听到他笑在6月中旬有时候，我坐在，我不知道 i reminisce 我觉得所有好的时候，我们已在我们的生活有时候，我不能超过如何美好的那些日子回到我们的谎言简单的日子简单的时代

Uh Uh, uh, huh Yo, yo Drop your glasses, shake your asses Face screwed up like you having hot flashes Which one, pick one, this one, classic Red from blonde, yeah bitch Im drastic Why this, why that, lips stop askin Listen to me baby, relax and start passin Expressway, hair back, weavin through the traffic This one strong should be labeled as a hazard Some of yall niggas hot, sike Im gassin Clowns I spot em and I cant stop laughin Easy come, easy go, E-V gon be lastin Jealousy, let it go, results could be tragic Some of yall aint writin well, too concerned with fashion None of you aint gizell, cat walk and imagine Alotta yall Hollywood, drama, passed it Cut bitch, camera off, real shit, blast it And if I had to give you up Its only been a year Now I got my foot through the door And I aint goin nowhere It took awhile to get me in And Im gonna take my time Dont fight that good shit in your ear Now let me blow ya mind They wanna bank up, crank up, makes me dizzy Shank up, haters wanna come after me You aint a ganster, prankster, too much to eat Snakes in my path wanna smile up at me Now while you grittin your teeth Frustration baby you gotta breathe Take alot more that you to get rid of me You see I do what they cant do, I just do me Aint no stress when it comes to stage, get what you see Meet me in the lab, pen and pad, dont believe Huh, sixteens mine, create my own lines Love for my wordplay thats hard to find Sophomore, I aint scared, one of a kind All I do is contemplate ways to make your fans mine Eyes bloodshot, stressin, chills up your spine Huh, sick to your stomach wishin I wrote your lines And if I had to give you up Its only been a year Now I got my foot through the door And I aint goin nowhere It took awhile to get me in And Im gonna take my time Dont fight that good shit in your ear Now let me blow ya mind Let your bones crack Your back pop, I cant stop Excitement, glock shots from your stash box Fuck it, thugged out, I respect the cash route Locked down, blastin, sets while I mash out Yeah nigga, mash out, D-R-E Back track, think back, E-V-E Do you like that, you got to I know you Had you in a trance first glance from the floor too Dont believe Ill show you, take you with me Turn you on, pension gone, give you relief Put your trust in a bomb when you listen to me Dancin much, get it all?

Uh Uh ， uh，哼！ Yo， yo 降低你的眼镜，摇动你的驴脸振作起来像你有热的闪光哪一个，精选一，这一个，古典作品红色的从金发碧眼的女人，是的母狗 Im 激烈的为什么这，为什么那，唇停止 askin 听我的话宝贝，放松而且开始 passin 高速道路，头发背面，透过交通的 weavin 这一个强壮的应该被贴标签为危险一些 yall niggas 热的， sike Im gassin 我弄脏 em 的小丑和我讲黑话停止 laughin 易得易失，电子 V gon 是 lastin 妒忌，让它去，结果可能是悲惨的一些 yall aint writin 很好地，太关心的由于流行你没有一个 aint gizell，猫走而且想像 Alotta yall 好莱坞，戏剧，通过了它削减母狗，照相机走开，真正的粪，摧残它而且如果我必须放弃你它的唯一是一年现在我使我的脚通过门而且我无处的 aint goin 它一会儿拿让我获得录取而且 Im 去从容不迫 Dont 在你的耳朵中对抗好粪现在让我吹 ya 思想他们想要存入银行在，上面曲柄提高，使我晕眩胫向上，憎恨者想要跟随我你 aint ganster，玩皮的人，太很多的吃在我的路径的蛇想要仰头对我笑现在当你的时候 grittin 你的牙齿挫折纵容你必须呼吸带 alot 更多你免除我你见到我做他们所讲黑话的做，我仅仅做我 Aint 没有压迫力当它来举行的时候，拿你见到的在实验室，钢笔和填补中遇到我， dont 相信哼！，十六我的，产生我自己的线为我的文字的争论爱那难者找二年级学生，我 aint 惊吓，一类型全部我做是注视方法使你的狂热者成为我的眼睛充血的， stressin，寒冷在你的背骨上面哼！，你的胃 wishin 的病人我写了你的台词而且如果我必须放弃你它的唯一是一年现在我使我的脚通过门而且我无处的 aint goin 它一会儿拿让我获得录取而且 Im 去从容不迫 Dont 在你的耳朵中对抗好粪现在让我吹 ya 思想让你的骨头裂缝你的背部取出，我讲黑话停止刺激， glock 注射从你的藏起来盒子性交它，宗教性暗杀团员出，我尊敬现金路径锁了下来， blastin ，组当我调情出的时候是的 nigga，调情出， D-R-E 支持轨道，向后地想，电子 V-E 做你相似的，你到达我认识你也在来自地板的梦幻之境第一一瞥中有了你 Dont 相信生病的表演你，和我带你把你打开，发给退休金离去，给你减轻当你听我的话时候，把你的信赖放入一个炸弹 Dancin 很多，拿它全部？

Today require students to complete a chlorinated paraffin, when the number of species, there are very few students have forgotten any judgments, although I put the time in class to determine the method to write on the blackboard, two; but the face of such When the situation, I did not angry, but a very patiently to determine ways to re-explain it to them in detail, though I talked to repeat 5 times, and to account for the time in learning the key to knowledge of the record class.

今天在要求学生完成烷烃的一氯代物种数的时候，有很几个学生都已经忘记任何判断了，虽然我在上课的时候就把判断的方法在黑板上书写了两次；但面对这样情况的时候，我没有生气，而是很耐心地把判断方法再次详细地讲解给他们听，虽然我重复讲了5次，并交代他们在学习的时候该记录上课的关键知识。

Nobody loves you when you're down and out [00:18.29]Nobody sees you when you're on cloud nine [00:26.92]Everybody's hustlin' for a buck and a dime [00:35.16]I'll scratch your back and you scratch mine [00:44.54]I've been across to the other side [00:52.89]I've shown you everything, i got nothing to hide [01:01.46]And still you ask me do i love you, what it is, what it is [01:11.71]All i can tell you is it's all show biz [01:19.34]All i can tell you is it's all show biz [01:32.64]Nobody loves you when you're down and out [01:41.31]Nobody knows you when you're on cloud nine [01:49.88]Everybody's hustlin' for a buck and a dime [01:58.67]I'll scratch your back and you knife mine [02:07.40]I've been across the water now so many times [02:16.16]I've seen the one eyed witchdoctor leading the blind [02:24.87]And still you ask me do i love you, what you say, what you say [02:33.11]Everytime i put my finger on it, it slips away [02:41.46]Everytime i put my finger on it, it slips away [03:26.88]Well i get up in the morning and i'm looking in the mirror to see, ooo wee!

当你落魄潦倒的时候，没有人爱你 [00：18.29] 当你是在云九上的时候，没有人看见你 [00：26.92] 每个人 hustlin'为一块钱和一个一角硬币我将擦你的背面和你擦伤的我的 [00：44.54] 我有是横过到另一边我有显示你每件事物的， i 什麽也不得到到兽皮我做 i 的你问和剧照爱你，它是什么，它是什么所有的 i 能告诉你的是它所有的是演艺事业所有的 i 能告诉你的是它所有的是演艺事业 [01：32.64] 当你落魄潦倒的时候，没有人爱你 [01：41.31] 当你是在云九上的时候，没有人认识你 [01：49.88] 每个人 hustlin'为一块钱和一个一角硬币我将擦你的背面和你刀的我的 [02：07.40] 我有是横过水现在如此许多次 [02：16.16] 我有看眼的 witchdoctor 领先盲人我做 i 的你问和剧照爱你，你说的事情，你说什么 Everytime i 把我的手指放在～上它，它滑倒离开 Everytime i 把我的手指放在～上它，它滑倒离开好的 i 在早晨起床的和我正在顺便探访镜子看见， ooo 很小的！

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。