在....末端

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

I had another belt not so broad, and fastened in the same manner, which hung over my shoulder; and at the end of it, under my left arm, hung two pouches, both made of goat's-skin too; in one of which hung my powder, in the other my shot: At my back I carried my basket, on my shoulder my gun, and over my head a great clumsy ugly goat-skin umbrella, but which, after all, was the most necessary thing I had about me, next to my gun: As for my face, the colour of it was really not so Moletta-like as one might expect from a man not at all careful of it, and living within nine or ten degrees of the equinox.

另一条皮带斜挂在我的肩膀上，它不那么宽，也是用同样的方法系上的；它的末端，也就是在我左胳膊下，挂着两个袋子，也是羊皮做的，一个装着火药，一个装着子弹。我背上背着筐子，肩上扛着枪，头上撑着一把笨拙丑陋的羊皮大伞。不过这伞毕竟是我随身最需要的东西，仅次于枪。至于我的脸，并不像一个住在赤道九度、十度以内地区不修边幅的穆拉托人（原文为moletta-like）那样黑。

I had another Belt not so broad, and fasten'd in the same Manner, which hung over my Shoulder; and at the End of it, under my left Arm, hung two Pouches, both made of Goat's-Skin too; in one of which hung my Powder, in the other my Shot: At my Back I carry'd my Basket, on my Shoulder my Gun, and over my Head a great clumsy ugly Goat-Skin Umbrella, but which, after all, was the most necessary Thing I had about me, next to my Gun: As for my Face, the Colour of it was really not so Moletta-like as one might expect from a Man not at all careful of it, and living within nine or ten Degrees of the Equinox.

另一条较窄的皮带，斜挂在我的肩膀上，也用皮条系着。这条皮带的末端，在我左胳膊下，挂着两个山羊皮袋，一个装火药，一个装子弹。我背上背着筐子，肩上扛着枪，头上撑着一顶羊皮做的大阳伞，样子又难看又笨拙。尽管如此，除了枪之外，这把伞也是我随身不可缺少的东西。至于我的脸，倒不像穆拉托人那么黑，看上去像一个住在赤道九度、十度之内的热带地区那种不修边幅的人。

Flare-Tip The flare-tip is typically a "shaft-over" hosel, meaning the shaft covers the top of the hosel where the two connect.

Flare-Tip：该特点在於杆颈插在杆身内，也就是说杆颈的最上端是在杆身末端内。

This latter organ is external and situated at the extremity of the trunk; it is composed of two separate parts: of which the extreme part is fleshy, does not alter in size, and is called the glans; and round about it is a skin devoid of any specific title, which integument if it be cut asunder never grows together again, any more than does the jaw or the eyelid.

后面的器官是在外面的而且位于躯干的末端；它由独自两部分组成：它外面的部分是肉质的，不能改变尺寸，被称作龟头；围绕在它周围的是一层缺乏名称的皮肤，如果这层覆盖物被切开，就绝不能再长合在一起。

Fruit globose to ellipsoid, rounded at both ends, slightly compressed laterally, mericarp nearly rounded in cross section, commissural face constricted, glabrous; ribs 5, prominent, acute; vittae 1 in each furrow, 2 on commissure.

果球状到椭圆形，在两末端圆形，侧面稍压扁，分果爿近圆形在横断面上，接合面缢缩，无毛；棱5，突出，锐尖；油管每棱槽1，在合生面上2。

Squatting, lying, and milling aimlessly about the lower level were a dozen of so large lizard-like beasts that looked like the product of some mad geneticist's cross of Tatooine krayt dragons wit h Haruun Kal ankkoxen : four meters tall at the shoulder, long crooked legs that ended in five-clawed feet clearly designed for scaling rocky cliffs, ten meters of powerful tail ridged with spines and tipped with a horn-bladed mace, a flexible neck leading up to an armor-plated head that sported an impressive cowl of spines of its own—they looked fearsome enough that Obi-Wan might have thought them some sort of dangerous wild predators or vi cious watchbeasts, were it not for the docile way they tolerated the team of Utai wranglers who walked among them, hosing them down, scraping muck from their scales, and letting them take bundles of greens from their hands.

在下层那儿，蹲着的，躺着的，或是在漫无目的地殴斗的，是整整一打类似蜥蜴的巨型野兽，它们看上去就像是某个科学狂人将塔图因的克雷特巨龙和哈伦·卡尔的安科森兽糅合在一起的成品：肩高四米，长而弯曲的腿的末梢长着五根用于攀登岩壁的爪子，十米长的强壮尾部上扎满了尖刺，末端是一个尖角满布的骨槌，柔韧的颈部支撑着一个布满铠甲的脑袋，脑袋上又炫耀着一簇可怕的尖刺——它们的长相实在太恐怖了，使得欧比旺不禁觉得这些家伙不是危险的野生掠食者，就是冷血的守卫神兽，可恰恰相反，它们在那些尤泰牧人面前却表现得十分乖巧温顺，任由他们从中穿行，往它们身上浇水降温，从它们的鳞片上刮去污物，以及将一捆捆青草递到它们嘴边。

Two known genes lie within the implicated genetic interval. One is FTO (a locus associated with fat mass and obesity), which has been implicated in nucleic acid demethylation and is highly expressed in the arcuate nucleus of the hypothalamus; the other, called fantom, or FTM (KIAA1005 or RPGRIP1L), begins transcription at a location only 200 base pairs upstream from the 5' end of FTO. FTM is a structural component of the ciliary body, present on all cells, that plays a role in a wide range of cellular and organ functions.9 Elements of the cilium are mutant in Bardet Biedl syndrome, which includes obesity among its characteristic phenotypes.10

有两个已知的基因存在于基因间隔中，一个是FTO（与脂肪体积和肥胖相关的基因位点），它在核酸脱甲基中发挥作用并且在下丘脑弓状核中高表达，另一个称作FTM，其在FTO 5'-末端的上游序列仅200个碱基对中开始转录，FTM是睫状体的结构组成部分，在所有细胞中都要表达，并在细胞和器官功能中发挥广泛的作用。

The nexu's broad face was split by a terrifying tooth-filled maw, and four eyes glinted from atop its spade-shaped head. Its wiry, supple muscles were visible beneath its fine coat, and sharp curving quills lined its spine. A naked, forked prehensile tail trailed it, aiding in balance, and its arms and legs terminated in large curved claws.

它宽阔的脸被一张充满牙齿的可怕大嘴撕裂开来，四个眼睛在它铲子状的头上闪闪发光，强韧、柔软灵活的肌肉在它美丽的皮毛下清晰可见，弧形的尖刺排列在脊椎上，后面还拖著光秃秃、分岔、适於抓握且有助平衡的尾巴，在四肢的末端有著大而弯曲的爪子。

On the bases of investigating and grasping the materials about the AHPPEs, AHPPEs' developing situation is analyzed completely in this article on vertical direction of time order and horizontal direction of department, in the meantime, material buying process, capital's using rate, and product selling process are also analyzed completely. Pointing out because the differences of owning right among State-Operated Enterprises, Collectively Operated Enterprises and Privately Operated Enterprises, there are differences in the producing behavior and trade development.A. According to the theory of producer behavior, POEs is on the way from stageⅠto stage Ⅱ,economic interests increasing;COEs is on the end of stageⅡ,economic interests decreasing;SOEs on stage Ⅲ,economic interests is negatively increasing.Model analyzing method shows that scale economic doesn't exist in SOEs and COEs, but POEs is the reverse.B. Different owning right enterprises have great differences in the respect of capital scale,product making and selling processes, at last SOEs and COEs' economic interests is much less than that of POEs. Scale economic doesn't exist in SOEs and COEs, POEs have the potential of developing scale economic, furthermore, the new-coming horizontal in the horizontal structure have the good situation.

在跟踪调查掌握资料的基础上，文章对该区农畜产品加工企业的发展状况从时间序列的纵向和行业结构的横向进行了比较分析，同时又从原材料购买过程、资金的利用效率以及产品的销售过程等各个方面进行了全方位地剖析，指出由于企业存在着国有、集体和民营产权类型的不同，导致生产行为和行业发展存在着差异：A、生产者行为理论分析总结出，民营企业正在处于第Ⅰ阶段向第Ⅱ阶段的过度区，经济效益上升；集体企业处于第Ⅱ阶段的末端，经济效益下降；国有企业处于第Ⅲ阶段，经济效益负增长。B、不同产权企业在资金规模，产品的生产和售卖过程上存在着很大不同，最终导致国有和集体企业经济效益比民营企业要差得多，用模型方法分析得出结论：国有企业和集体企业都不存在规模经济，而民营企业存在规模经济的发展潜力，并且在行业结构中属于民营产权的新兴行业发展状况良好。C、行业间也存在着很大的不同：集体企业的优势行业是制酒、粮食加工和肉食品加工等行业；民营企业的优势在皮件类、服装、粮食加工和制酒等行业；国有企业当中食品加工、肉食品加工和食品冷库类是优势行业。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。