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Then the pile-soil stress characteristics and stabilizing mechanism of the composite foundation are fully discussed. Based on the close analysis of the influence factors of the vertical bearing capacity, circular aperture extension theory is employed to establish the computing formulas for the pile vertical stress and the foundation vertical bearing capacity, respectively. Moreover, with analyzing the features of the calculation parameters such as materials of gunite mortar, jet pressure, diameter of the jet pipe, pile length, pile layout, a design theory for chemical churning pile composite foundation is proposed. Then, the variation of the pipe deformation with loads, the deformation coordination of pile and soil are studied. And methods to decide pile-soil stress ratio and the compressive modulus are developed. Meanwhile, the corresponding design method and the design parameters are proposed. In addition, studies for construction technique and quality detection of the chemical churning pile composite foundation are presented.

在此基础上深入探讨了旋喷桩的桩土受力特性及其复合地基的加固机理,从影响旋喷桩复合地基竖向承载力的因素入手,基于圆孔扩张理论给出了桩体竖向应力及旋喷桩复合地基的竖向承载力计算公式;通过对喷射浆液、喷射压力选取、喷射直径估算、桩长及桩位设计等各种计算参数的深入分析和研究,提出了一套相应的旋喷桩复合地基设计计算方法;通过计算分析,深入探讨了旋喷桩复合地基变形随荷载变化规律、桩和桩间土变形协调关系、桩土应力比及复合地基压缩模量的确定,并提出相应的设计方法和修正参数;此外,结合工程应用,对旋喷桩复合地基的施工技术及其现场质量检测方法进行了较全面的探讨。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下:一、tau蛋白过度表达和聚积对细胞形态的影响:倒置显微镜下观察两种细胞的形态,发现N2a/wt细胞的突起多而长,而N2a/tau40细胞胞体变圆,突起明显缩短;免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍,免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光,tau主要分布在核周和突起起始部分的胞质内,而N2a/wt细胞内的荧光很弱。

Measured the basic physical parameters of unsaturated soils in Three Gorges Reservoir area; 2 measured the initial sample matric suction using the unsaturated triaxial testing system, which can be used to evaluate the unsaturated soil strength and stress; 3 the drying and moisture absorption experiments were executed using the Tempe apparatus, and got the Soil-Water Characteristic Curve, permeability coefficients of every stress level were simulated according to the parameters from the SWCC; 4 the unsaturated soil strength tests were carried out using the GDS triaxial testing system, then got the Mohr circle and shear strength parameters; the results from different stress paths were compared; meanwhile, the axial strain-stress ratio-volume strain curve of different matric suction and different radial pressure was got, as well as their relationship; The research, which provided the necessary data and reference for Three Gorges Reservoir area geologic disaster, is the basic component of Three Gorges Reservoir geologic disaster prevention and research, and experimental results will be used directly in the project.

1量测了三峡库区非饱和土的基本物性参数; 2用非饱和土三轴试验仪量测了试样的初始基质吸力,为评估非饱和土的强度和应力水平提供参考; 3用Tempe仪和体积压力板仪进行了非饱和土的干燥和吸湿试验,得到了土-水特征曲线,并根据其参数拟合出各级吸力状态下对应的渗透系数; 4用GDS三轴仪进行了强度试验,得到了Mohr圆和抗剪强度参数;并比较分析了不同应力路径下试验结果;同时得到不同吸力和不同围压下轴向应变-应力比-体应变的关系曲线和其相互关系。该研究是三峡库区地质灾害防治研究工作的基础性工作,其结果将直接应用到工程实际中,为三峡库区地质灾害提供必要的数据和参考。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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