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NGF had obvious cytotoxicity on these cell lines ,which shows to be dose-dependent, especially for MGC-803 and BEL-7404 ,their IC_(50) value at the 24~ hours are 49.9mg/L and 55.46mg/L ; Main features of apoptosis under fluorescent microscope include reduction in volume,nuclear chromatin condensation, intensification under jacinth fluorescence; The early apoptosis rate of pristine cells which detected by FCM increases with NGF dose increasing.

2NGF对4种人肿瘤细胞均有细胞毒性作用,且呈剂量依赖关系,尤其是对MGC-803和BEL-7404的抑制作用更强,作用24h的IC50值分别为49.9mg/L和55.46mg/L,荧光显微镜下可观察到MGC-803凋亡细胞的主要特点为:细胞体积缩小,染色质固缩,橘红色荧光增强;流式细胞仪检测到MGC-803细胞的早期凋亡率随NGF浓度的增高而增加,各浓度组与对照组的早期细胞凋亡率比较有明显差异(P<0.01)。

The Ni5Zr with low melting point forms because of Zr-rich liquid film will be maintained below 1180℃, therefore, solidification range of the alloy widens by 80℃ and the intenyal between the temperature at which the interdendritie feeding action disappears and the solidus temperature enlarges by 60℃....

在凝固过程中富Zr的液膜保持到低于1180℃的温度,结果使合金的凝固范围扩大了80℃。使失去枝晶间补缩的温度与固相线的温度范围扩大了60℃。这是含Zr含金热裂倾向大的原因之一。

In hypothalamic median eminance of kidney Yang deficiency group, the bounds of ependymocytes were dim, distribution of their microvillus changed, the top of microvillus enlarged and became spherical, and holes occurred on the surface of the cells. In that of control group, the cell arranged in order and the cell bouns were clear, the microvillus were equal in thickness and in long, thin acerose thread wool shape. The surface ultrastructure of ependymocytes on hypothalamic median eminance in herb group was similar to that of control group.

肾阳虚大鼠垂体内分泌细胞和甲状腺细胞存在着不同程度的粗面内质网、高尔基体扩张,线粒体空化,细胞变形,核固缩等超微结构的损伤;中药组大鼠垂体超微结构与正常组类似,细胞形态完整,结构清晰,细胞器结构正常,含有各种大小不等、形态不一的分泌颗粒,但甲状腺细胞超微结构与肾阳虚组相比未见明显改善。

Results: Neonatal rats in hypoxic-ischemicgroup behaved restlessness, cyanosis, deep and rapid breath, astasia, lethargy, irritation and spasm; There were local pyknosis, fragmentation, dissymmetry, blur or disappearance of nucleoli, raritas of ground substance in left cerebral cortex; but there was no statistic significance in chinese traditional medicine group and sham group. The expression of HIF-1α mRNA in brain tissue of experimental group and chinese traditional medicine group were higher than that in sham group, especially chinese traditional medicine group at 3d; HIF-1α positive cells were found in cerebral cortex and hippocampus. There were more HIF-1α positive cells in experimental group and chinese traditional medicine group and mainly in vascular endothelial cells.

结果 缺氧缺血后大鼠相继表现为烦躁不安、全身发给、呼吸加深加快、站立不稳、嗜睡、激惹或间断发作的痉挛和抽搐;HE染色显示实验组大鼠左侧大脑皮层出现局灶性神经元核固缩,核碎裂,核仁偏位,不清或消失,基质疏松;中药组大鼠脑组织结构基本接近假手术组,无明显水肿和坏死表现;实验组和中药组大鼠脑组织HIF-1α mRNA表达较假手术组明显增加,尤其是中药组实验第3日者;免疫组化显示实验组和中药组各时间点大鼠大脑皮层和海马区均可见不同程度的HIF-1α表达,明显高于假手术组,阳性表达主要在血管内皮细胞,海马和皮层的锥体细胞亦有HIF-1α阳性细胞分布。

Results ⑴ The cell proliferation was inhibited by TET. It showed thatdivision index and cell number during G0-G1 were decreased, while S and G2-M phases were lengthened with dosage and time dependency in dosages of TET from 2mg/L to 8mg/L; besides, oval-shap cells, karyopyknosis and fragmentation were observed under light microscope. The inhibitory effect in T2 group was statistically similar to that in triamcinolone.

结果⑴ TET在2mg/L~8mg/L之间呈剂量及时间依赖性地抑制瘢痕成纤维细胞的增殖,使生长曲线不同程度的压低、分裂指数降低、G0-G1期细胞数减少、G2-M期增多、S期与G2-M期延长,光镜下见细胞突起变短,细胞由梭形变成椭圆形,核固缩、碎裂。P.C的改变近似于T2组(4mg/L TET)。

Studies on the regulation of malignant phenotype expression in a mouse plasmocytoma cell line (SP2/0) by means of cellular engineering techniques of cell hybridization between the fusion of the mouse myeloma cells with rat intermediate or late erythroblasts.

三、利用细胞杂交的细胞工程手段,首次系统地研究了大鼠中幼、晚幼成红细胞与小鼠浆细胞瘤(SP2/0)细胞融合后恶性表型的表达状态,分析自然去核前红细胞胞质对骨髓瘤细胞恶性增殖的调控作用,以及晚幼期固缩核重新被激活的可能性。

The cytologic features of normal squamous epithelial cells can be seen at the center top and bottom, with orange to pale blue plate-like squamous cells that have small pyknotic nuclei.

在涂片的中央上方和底部我们可以观察到正常鳞状上皮细胞的细胞学特征,鳞状细胞呈圆形,胞浆呈橘黄色,小的淡蓝色的固缩核。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中,雏鹅临床表现为高发病率和高死亡率,食欲和饮水减少,拉白色、黄绿色稀粪,伴有神经症状;常规组织学观察,脾脏和胰腺肿大,有白色坏死斑点,肠道出血和坏死,肝脏肿大变性,肾脏肿大变性有尿酸盐沉着等;用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究,结果表明:胰腺中发现病毒包涵体,肠道中大量纤维素以及胶原纤维增生,肾脏、肺脏大量含铁血黄素沉着,免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少;电镜下多种细胞呈现线粒体水肿,多种实质细胞的染色质固缩,染色质边集,特别是细胞内容物如脂肪滴增多的病理变化特征。

At day 20, some of the acini collapsed, and the epithelial cells of the duct were damaged.

由10μg·L-1 B\P处理20d时,在鳃回血管中发现血细胞坏死后残留的固缩细胞核,鳃丝的上皮细胞排列不规则甚至断裂,部分消化腺泡崩解,鳃丝结构和消化导管上皮结构损害严重;消化盲囊组织结构的损伤比鳃更为严重。

Result vitamin A and 4HPR fall to Hela cell collect form have apparent inhibition, can restrain Hela cell to be opposite the action causing tumor of naked rat (the curb tumour action of P.01); vitamin A issues visible cell solid by force to shrink at 4HPR(P.01); report lens, nuclear film is screwy, nuclear chromosome gathers agglomerate stand by the wither such as nuclear film to die DNA of cell of cellular feature; by degradation, a collection of illustrative plates of typical " of shape of " a flight of stairs appears in electrophoresis of agaragar candy gel.

结果维生素A和4HPR对Hela细胞集落形成有明显抑制功能,并可抑制Hela细胞对裸鼠的致瘤功能(P.01);维生素A的抑瘤功能强于4HPR(P.01);电镜下可见细胞固缩,核膜扭曲,核染色体聚集成块并靠近核膜等凋亡细胞特征;细胞DNA被降解,在琼脂糖凝胶电泳中呈现典型的&阶梯状&图谱。

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