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In the normal group, electron microscopy showed normal chondrocytes were oval, and cells and cell membrane were intact. In cytoplasm, there were abundant rough endoplasmic reticulum, dictyosome, and mitochondria with intact nucleus, and even chromatin. In the model group, chondrocytes exhibited obvious pyknosis and their shape was irregular. The areolae around cells disappeared; cell organs in cytoplasm coagulated to flaps which showed electron dense and was uneasy to observe; the shape of nucleus showed irregularity, and caryotin gathered densely.

电镜下正常对照组软骨细胞呈椭圆形,细胞及胞膜完整,包质内可见丰富的粗面内质网、高尔基体、线粒体,细胞核完整,染色质分布均匀;模型对照组软骨细胞明显固缩且外形不规则,细胞周晕消失,胞浆内细胞器凝成高电子密度的片状物不易分辨,细胞核不规则,染色质浓聚,散裂于核中。

The resluts indicated that the ideal effects had been proved with enzymatic hydrolysis of mixing 2% cellulose enzyme and 0.5% pectic enzyme for 40~60 min, then pycnosis treatment in ice water for 20~30 min after post-hypotonic treatment, and carbol fuchsin solution dyeing.

结果表明:用2%纤维素酶与0.5%果胶酶的混合液处理40~60 min,后低渗处理之后进行20~30 min冰水固缩处理和卡宝品红染色组合可以得到理想的制片效果。

Control cells appeared to be mostly round,intact and bright green while a lot of the cells cultured with GM exhibited some irregular cell morphology ,with bright green or jacinth nuclei , Pyknosis and fragment appearance which characteristic apoptosis .

GM损伤组作用24小时后,可见较多的核着亮绿色或橘红色、呈固缩状或片段状的凋亡细胞,当时间延长到48小时,凋亡细胞进一步增多。

The staining of brush cells of experimental group showed nuclear shrinkage, margination, nuclear fragmentation and formation of apoptotic bodies, and the characteristic ladder band on gel electrophoresis. NO changes were observed in control group.

实验组细胞刷片染色显示有明显的细胞核固缩、核周边染色质加深、核断裂、凋亡小体现象,细胞DNA电泳可见梯状条带的凋亡特征,而空白对照组无此现象。

Results1.In diabetic rats,the pathological change of the retinal structure displayed the decrease of the retinal ganglion cells\' number,and the signs of the apoptosis in different degrees could be found in the altrastructure of retinal neurons,including:cell shrinkage,chromatin margination and nuclear collapse,and so on.

结果1。随糖尿病大鼠病程延长,逐渐出现视网膜神经节细胞数量减少、细胞排列紊乱的病理改变,透射电镜下可见视网膜神经细胞出现胞浆浓缩、细胞体积缩小、染色质边聚、细胞核固缩、断裂等不同阶段的凋亡征象。

RESULTS AND CONCLUSION: After exercise, apoptosis occurs in normal muscle or muscle under pathologic state. The apoptosis of skeletal muscle cells is similar to common cell apoptosis, including karyopyknosis, plasma membrane vacuole, cell organ shrinking, and apoptotic body formation.

结果与结论:运动后,正常肌肉中或是病理状态下的肌肉中骨骼肌细胞都会出现凋亡,凋亡的形态学表现与普通凋亡细胞相似,即核固缩、质膜发泡、细胞器紧缩,凋亡小体形成,其凋亡过程大致可分为3个阶段,即启始阶段、效应阶段和降解阶段。

ResultsThe glandular cell in the endometrial implant after therapy with middle or high-dose of (10,15 gkg-1d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size, karyopyknosis , cytoplasmic and nuclear chromatin condensation , density increased and a lot of apoptotic bodies among cell. Whereas some stromal cell displayed degeneration and necrosis. The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.

结果中、高剂量小柴胡汤(10,15 gkg-1d-1)治疗后,异位内膜有较多腺上皮细胞出现凋亡的特征,表现为细胞体积变小,核固缩,胞浆和核染色质凝集,密度增高,细胞间凋亡小体,同时间质细胞中可见一些坏死细胞;异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。

The proliferating cells were mainly distributed in the peri-chondrium and the peripheral zone of tracheal cartilage. The senescent chondrocytes were distributed in the central zone whereapoptotic morphological characteristics obviously appeared, such as pyknosis, karyorrhexis.

结果:软骨细胞呈现多种方式的无丝分裂像;气管软骨膜与软骨周围带多见细胞增生;气管软骨中央带多见衰老的软骨细胞,并见明显核固缩、核碎裂等细胞凋亡形态学特征。

Morphologically, the macrophages treated with NO and O-LDL presented shrinkage, nucleus fragmentation, and cytoplasm condensation; DNA ladder was visualized by agarose gel electrophoresis; Characteristic apoptosis peak of macrophages was showed by flow cytometry; Ultrastructure, The chromatin condensed and marginated to form dense chromatin fragmentation; The cytoplasm concentrated and vacuolized; The lysosomes and mitochondria of apoptosis macrophages increased clearly.

电镜观察超微结构变化:染色质固缩边聚,形成致密、均质的染色质块,胞质浓缩、电子密度升高并空泡化;凋亡巨噬细胞溶酶体、线粒体增多;细胞突起缩短减少,但巨噬细胞特有的板状伪足不消失。

After 48h exposure to 50mg/L ox-LDL, under the fluorescent microscope partial cells Showed typical apoptotic morphological changes such as nacarat fluorescent body shrinking and fragment of the nucleus. At the same time, the cellular cholesterol content also increased obviously, and many lipid droplets accumulated in cells.

RAW264.7细胞经50mg/L ox-LDL处理48h后,AO/EB染色显示明显的细胞凋亡形态学特征,出现核固缩、碎裂,同时细胞内胆固醇含量显著增加(由76.3μg/mg蛋白上升至368.5μg/mg蛋白),油红O显示细胞内有大量的脂滴存在。

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