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The ultramicrostructure in atrial myocytes displayed glycogen aggregation, karyopycnosis, sarcoplasmic reticulum distention and chondriosome swelling at 24 hours after rapid pacing.

经过24 h的快速起搏,心房肌细胞出现糖原聚集、核固缩、空泡样变以及肌浆网扩张、线粒体肿胀、部分线粒体嵴轻度溶解等超微结构的改变。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果:细胞接触肽段后存活率下降28.9%;光镜下可见细胞贴壁不良,胞体缩小,细胞突起断裂缩短;荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞;电镜下可见胞质中出现空泡样结构,细胞染色质浓集于核膜内侧并裂解成碎块状;流式细胞仪检测细胞出现亚二倍体峰,DNA电泳出现梯状带。

With immature embryos of Citrus grandis'Guanximiyou' as material, the effect of the three factors, enzyme treating time, pycnosis treating time in ice water and dyeing, were studied in the process of single chromosome microdissection technique.

以'琯溪蜜柚'幼胚为材料,对单染色体微分离制片过程中酶解时间、冰水固缩时间和染色三因素的影响效果进行了研究。

Results:The degenerative changes of articular cartilage in the manipulation group and the sodium hyaluronate group,such as vacuolar degeneration of cytoplasm,decreased quantity and degraded function of orginele,pycnosis of nuclear and necrosis even disintegration of cell,are lesser than that in the group without treatmnet.

结果:手法干预组和透明质酸钠组软骨细胞发生胞浆空泡化,细胞器数量减少,功能减退以及细胞核固缩甚至细胞坏死崩解等退变现象的程度较空白对照组轻,手法组与透明质酸钠组相当。

RESULTS On HE staining, acinar cells in the pancreas showed pyknotic nuclei and formation of apoptotic bodies, which is the typical morphological features of apoptosis.

结果 HE染色观察到胰腺组织中典型的细胞凋亡形态学特征:细胞核固缩及凋亡小体形成。

Light microscope inspection in MAN, DXM and BC groups showed lesse ned brain edema, slight increase of perivascular space, and occasional nerve ce ll pyknotic nucleus.

MAN,DXM,BC组脑组织水肿程度较B E组减轻,仅见少量的血管周围间隙增宽,偶有神经细胞核固缩,DXM组无神经细胞空泡样变性。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400

Results: Compared with that treated with anti-thyroid drugs alone, after the combined therapy, the size of thyroid decreased significantly, P<0.01. Typical apoptosis appearance, such as vacuolated cell, marginal nuclei, chromatin condensation and nuclear fragmentation, could be seen under light microscopy. The apoptotic rate before and after adding Chinese herbs was (2.11±1.78)%and (18.66±20.01)%respectively (P<0.01). TUNEL positive cells were detectable for up to 2-20 weeks after Chinese herbal treatment.

结果:与单纯抗甲状腺药物治疗比较,患者经抗甲状腺药物与中药联合应用治疗2-10周后,甲状腺组织明显缩小(P<0.01);光镜下可见细胞发泡、核着边、核固缩、核小体、核分裂等凋亡细胞的典型形态;加中药治疗前后甲状腺细胞凋亡比率分别为(2.11±1.78)%和(18.66±20.01)%(P<0.01);末端标记呈阳性。

Simultaneously the disappearance of microvilli, vacuolization of cytoplasm, dispersion of nucleus and appearance of apoptotic body were observed under transmission electron microscopy. All these morphological changes are hallmarks of apoptosis.

凋亡细胞经荧光染色后表现为细胞核固缩、碎裂;透射电镜下观察发现细胞微绒毛丧失,胞内出现空泡,胞核解体及形成凋亡小体等典型的凋亡特征。

Five hours after the noise exposure, the cochleae were harvested. mitochondria energetic function, succinate dehydrogenase activity, in the hair cells of cochleae was evaluated with a colorimetric assay using blue tetrazolium monosodium salt. the sdh-labeled organs of corti were double stained with propidium iodide, a dna intercalating fluorescent probe used to visualize the morphologic viability of hair cell nuclei.

采用琥珀酸脱氢酶(succinate dehydrogenase,sdh)染色法进行耳蜗基底膜细胞线粒体染色,细胞核dna荧光染料碘化丙啶(propidium iodide,pi)双重染色耳蜗基底膜,以未受噪声暴露动物为对照,显微镜下观察噪声暴露后耳蜗基底膜核固缩和核肿胀毛细胞琥珀酸脱氢酶染色的变化。

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