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To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中,苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变,并筛选在自生生长时期、共生生长时期的表型,以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因,我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法,发现lsrA基因主要在根瘤侵染区开始表达,表达时序也在侵染阶段左右,但晚于bacA基因表达;LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体,nifA和fixK基因的转录水平降低,nifH基因的转录被完全阻断,因此LsrA蛋白为根瘤发育所必需,是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因,并研究了它的调节特性。oxyR突变后,苜蓿中华根瘤菌对过氧化氢敏感性提高,适应性降低。

The paper reviews home and overseas research works on exhumation and use of nitrogen-fixing resource, genetics engineering of nitrogen fixation, and biochemistry of nitrogenase and chemical modeling of biological nitrogen fixation, and describe the prospect of biological nitrog...

本文从固氮资源的发掘和利用、固氮的遗传工程以及固氮酶生物化学和化学模拟生物固氮三方面对国内外的研究进展进行了全面的阐述,并对生物固氮研究的前景进行了展望。

The paper reviews home and overseas research works on exhumation and use of nitrogen-fixing resource, genetics engineering of nitrogen fixation, and biochemistry of nitrogenase and chemical modeling of biological nitrogen fixation, and describe the prospect of biological nitrogen-fixing research.

本文从固氮资源的发掘和利用、固氮的遗传工程以及固氮酶生物化学和化学模拟生物固氮三方面对国内外的研究进展进行了全面的阐述,并对生物固氮研究的前景进行了展望。

So biological nitrogen fixation has become one of the main directions for today's fertilizer development in the world.

生物固氮不仅固氮量大,而且还可提高土壤肥力,改善土壤条件,不污染环境;更为重要的是它有取之不尽,用之不竭的廉价氮源,成本低,利用率高,因此,生物固氮是今后肥料发展的主要方向之一。

Screened lines 1005,8502,2096,943,1454 and Dongnong 42 have high nitrogen fixation ability with their %Ndfa of about 70%.

大豆品系1005、8502、2096、1454、943和东农42是高固氮品系,它们的固氮效率高达70%,大豆品系1454和1555既高产又高固氮

All the results indicated that Brazilian sugarcane also had a nitrogen-fixing capacity in Guangxi, Mo was propitious to regulate nitrogenase activity of nitrogen-fixing bacteria in sugarcane under nitrogenous conditions, and promoted nitrogen fixing; while under non nitrogenous conditions, it was unpropitious to regulate the nitrogen activity and inhibited nitrogen fixation of nitrogen-fixing bacteria.

上述结果说明:巴西甘蔗在本地也具有一定的固氮能力;在不缺氮条件下,钼处理较利于调节甘蔗体内固氮菌的固氮酶活性,促进其固氮;而在缺氮条件下,钼处理不利于调节甘蔗体内固氮菌的固氮酶活性,抑制其固氮作用。

It was identified that the nif gene for aerogenic P. agglomerans strain waslocated on plasmid while for anaerogenic strains were located on chromosomes usingKlebsiella pneumoniae nifHDK as probe.

用nifHDK为探针进行Southern杂交证明固氮成团泛菌产气型的菌株固氮基因定位在质粒上,而不产气型菌株固氮基因定位在染色体上。

Nodulation, N\|fixing activity and total nitrogen fixation per plant of Coriaria sinica were positively correlated with leaf area and photosynthesis per plant of the host.

马桑植株的结瘤量、根瘤固氮活性和固氮能力均与植株叶面积和光合能力呈显著的直线相关关系,叶面积大、光合能力强的植株结瘤量大,根瘤固氮活性高,固氮能力强。

Uni-algal cultures were made first on media enriched with nitrogenous compounds to encourage vigorous growth.The uni-algal cultures were then subcul- tured onto nitrogen-free media,which effected a preliminary separation of those algae which could thrive in the absence of pre-f...

在100毫升无菌无氮培养基中生长四天的结果测定,水生686固氮蓝藻、水生678固氮蓝藻(A.azotica forma a)、水生670固氮蓝藻和水生508固氮蓝藻的固氮量分别为1.0146、0.938、0.8614和0.759毫克。

Uni-algal cultures were made first on media enriched with nitrogenous compounds to encourage vigorous growth.The uni-algal cultures were then subcul- tured onto nitrogen-free media,which effected a preliminary separation of those algae which could thrive in the absence of pre-formed nitrog...

在100毫升无菌无氮培养基中生长四天的结果测定,水生686固氮蓝藻、水生678固氮蓝藻(A.azotica forma a)、水生670固氮蓝藻和水生508固氮蓝藻的固氮量分别为1.0146、0.938、0.8614和0.759毫克。

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