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Methods: MII, GV and IVM-MII were vitrified. After thawed, they underwent in vitro maturation, in vitro fertilization, embryo cultivation or fixed for immunofluorescence. The rates of survival, maturation, fertilization, blastulation and cytoskeleton with normal configuration were calculated.

玻璃化冷冻生殖泡期卵、成熟中期卵及体外成熟卵,解冻复苏后,分别作体外成熟、体外受精、胚胎培养或固定作免疫荧光标记,统计复苏率、成熟率、受精率、囊胚率、细胞骨架正常率。

Methods: mⅱ,gv and ivm-mⅱ were vitrified. after thawed, they underwent in vitro maturation, in vitro fertilization,embryo cultivation or fixed for immunofluorescence. the rates of survival, maturation, fertilization, blastulation and cytoskeleton with normal configuration were calculated.

玻璃化冷冻生殖泡期卵、成熟中期卵及体外成熟卵ivm-mⅱ),解冻复苏后,分别作体外成熟、体外受精、胚胎培养或固定作免疫荧光标记,统计复苏率、成熟率、受精率、囊胚率、细胞骨架正常率。

It is advantageous not to break the back of vertebral ligaments ,provide 3D correction , the fixation is short, the internal fixation is stronger and the rate of vertebral healing is higher.

其优点是术中不破坏脊椎后方韧带,提供三维矫正,固定范围短,内固定较坚强,骨折愈合率高。

1Niche construction can lead to stable coexistence of diverse genotypes in spatially structured population, which supports a stable polymorphism even without heterozygote superiority.(2)With habitat deterioration, niche construction accelerates the formation of steady polymorphism and hence impedes the harmful influences of environment on the population, which might embody a life-history strategy of organism under the unfavorable environment.(3)Niche construction results in the coexistence with alternative polymorphism through genotype-environment feedback and limited gene flow.(4)The niche-constructing organism is an active force to alter its environment and hence the direction of natural selection in order to better survival.(5)Spatial dynamics and distribution pattern of metapopulation are profoundly influenced by time-lagged niche construction.(6)Metapopulation size can reach a fixed level in the recency effect and equal weighting of time lag but is statistical stability in primacy effect, which implies the primacy effect is most remarkable.(7)The increment in the relative weightingof each generation\' niche construction and the length of time lag are significant factors for system destabilization.(8)Moderate capacity of positive niche construction benefits the metapopulation persistence.(9)The narrowing of niche breadth can decrease the metapopulation size and thereby increase the extinction risk.(10)The coupled function of time lag and niche construction make the system oscillation and generate the spiral wave, spiral-broken and circular wave in heterogeneous habitat.The spatial distributions of metapopulation and resource content are complementary due to a phase lag of their both frequencies.(12)Metapopulation persistence with niche construction depends not only on the balance between colonization and extinction, but also on the balance between the ability of niche construction and natural dissipation of habitat.(13)Metapopula-tion can survive under certain condition when the percent of suitable patches in habitat is lower than the ration of extinction to colonization.(14)Two thresholds exist in the process of transition of habitat quality dynamics from unsuitable to suitable, which include the intensity of niche construction and the initial condition of system.(15)Metapopulation size is positive correlated with the ability of positive niche construction, which means that organism or population who has strongly positive influences on their environment plays an important role to maintain the available habitat.

通过上述几个方面的研究,主要得出以下15条结论:(1)生态位构建可使空间结构种群,甚至是在没有杂合子优势的条件下,形成多种可能的稳定基因型分布模式;(2)随着环境的破坏与恶化,种群的生态位构建作用加速其较早形成稳定多态以阻碍环境对种群的不利影响,解释了有机体在不利环境下的一种生活史对策;(3)生态位构建作用通过基因型—环境反馈机制及有限的基因交流导致基因型多态的稳定共存;(4)有机体的生态位构建作用是一种积极的动力改变环境进而改变其自然选择的方向;(5)生态位构建的时滞作用对集合种群动态和空间分布产生深刻影响;(6)集合种群大小在时滞的崭新效应和等权重效应下达到一固定值,而在首位效应下达到统计稳定,表明首位效应的影响最显著;(7)首位效应下生态位构建相对权重的增量和时滞的长度是影响系统不稳定性动态的关键因素;(8)适中的生态位构建强度有利于集合种群的续存;(9)生态位宽度变窄会减少集合种群的数量从而增加其灭绝风险;(10)时滞和生态位构建的双重作用使系统产生周期振荡,并在异质性环境中产生稳定的螺旋波,破碎的螺旋波和环形波三种分布模式;(11)集合种群与资源含量的空间分布因为相滞而互补;(12)具有生态位构建的集合种群续存不仅取决于侵占率与灭绝率之间的平衡,而且依赖于生态位构建能力同其生境的自然消耗速率之间的妥协;(13)当生境中适合侵占的斑块比例小于其侵占率与灭绝率之比时,生态位构建作用促使集合种群在一定条件下续存:(14)生境斑块的状态从不适合到适合转变过程中存在生态位构建强度和系统初始条件这两个阈值;(15)集合种群的大小同正生态位构建能力正相关,意味着对环境具有较强正作用的有机体对维持有效生境起积极作用。

The chest of fetal animals was opened, and the tissue was fixed with 4% paraformaldehyde at 4°C. Lungs from postnatal animals were inflation fixed at 25 cmH2O pressure via a tracheal cannula with the same fixative.

胸部胎动物开幕式,并组织固定, 4 %甲醛在4 ℃的温度肺从产后的动物通货膨胀率定为25 cmH2O 压力通过气管插管同固定。

Results The reposition rate reached 100% under X??ray after operation. And all the cases were followed up for 15.3 (6-36) months. No complications, such as wound infection, loosening of the internal fixation, breakage of the plate and redislocation or refracture after the removal of the plate, were observed.The shoulder joint function recovery was up to 97.14% according to Chen's evaluation.

结果 术后所有患者X线片评估复位率达100%,所有患者均获得随访,随访时间6~36个月,平均15.3个月;术后无一例发生伤口感染、内固定松动或断裂,内固定取出后无一例发生脱位和骨折,按Chen WJ疗效评价标准,肩关节功能恢复优良率97.14%。

GD trestle is the best fixing style for open fracture, but its stability is worse.TCP fits for the fracture of various parts,especially the fracture near joint.But there is higher rate of infection,non-union and refracture in TCP.GK nail should be the first treatment of malunion fracture or old fracture.

UADF最适用于严重开放性骨折,但稳定性差;TCP可应用于各部位骨折,尤其是靠近关节的骨折,但感染率、不愈合率及取钢板后再骨折率高;GK钉固定病人恢复快,并发症少,骨折愈合率高;最适合用于节段性及陈旧性骨折。

On the basis of vertebral pedicle screw fixation, using intervertebral bone grafting could increase the rate of fusion, and using isthmic and posterolateral bone grafting could add spine stability, with good efficacy.

在椎弓根钉内固定系统复位固定的基础上进行椎间植骨椎等制定间融合率高,峡部植骨及后外侧植骨融合后可增加脊柱的稳定性,疗效好,值得临床推广。

Within 6 months, all 40 patients with cervical plates had fusions, and the cervical lordotic alignment was increased (2.51±6.75), and without plates and bolt loose or break. Of the 30 patients without plates the cervical lordotic alignment were decreased (1.33±3.43)(P.01), 2 patients had not fusions within 6 months (the fusion rate was 93.3%).

植骨融合率和颈椎生理曲度:植骨加钛板内固定组术后6个月内植骨融合率100%,颈椎生理曲度维持良好,较术前增加(2.51±6.75)°,无钢板和螺钉松动或断裂;单纯植骨组术后6个月内植骨融合率93.3%,颈椎生理曲度较术前减少(1.33±343)°,两组比较差异有显著比(P.01), 2例术后3个月假关节形成。

Under the condition of laboratory incubation,the C loss of organicmaterials during their mineralization was greater than their N loss,inducing ahigher residual amount of organic organic N.During the decay of maize andrice staw with a wide ratio of C/N.soil microbes could fix N,but the fixationrate was quite low,only 0.2-0.9g N/assimulated 100g organic C.

在实验室培养条件下有机物料在矿化分解过程中C的损失总是大于N的损失,因此有机N的残留率明显高于有机C的残留;且由于灰分总量不变,有机物料的残留率也略高于有机C的残留率;〓比较宽的玉米秸和稻秸在腐解过程中发生了自生固N作用,但其固N效率仅为C:N=100:0.2-0.9,即每同化100g有机C,只能固定

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。