固定物

It is existed competitiveness inhibition of 6APA, non-competitiveness inhibition of PAA and substrate inhibition of Penicillin on the hydrolysis of benzylpenicillin to 6APA and PAA by an immobilized penicillin amidase. The heat conductive coefficients were determined on the vacuum elevation liquid membrane concentration. The thermodynamics and kinetics of PAA extraction were studied on the organic solvent extraction of the above solution.

研究证明固定化青霉素酰化酶裂解反应是典型的底物、产物抑制酶反应，其反应动力学数据表明，青霉素、苯乙酸以及6APA对反应分别存在底物抑制、非竞争性抑制和竞争性抑制；真空升膜浓缩适应热敏性以及粘度不大的物系，对于6APA水溶液的真空升膜浓缩过程研究得到了浓缩实验的传热系数；浓缩液萃取主要是提取出其中的苯乙酸，本文测定了不同温度以及溶剂条件下的苯乙酸溶解度，并观测了苯乙酸萃取动力学。

The complex of gold conjugate and analyte then moves onto the membrane strip and migrates towards the capture binding protein, where it becomes immobilized and produces a distinct signal in the form of a sharp red line.

金标和分析物的复合物扩散到膜上，并向检测蛋白移动，在那里复合物被固定住，产生清楚的信号——一条红线。

From this experiment, frequency response function could be found, and modal parameter also could be known by curve fitting, then compared with the differences of mode shape and natural frequency by using those modal parameters, also compared with finite element analysis, the results could be applied to detect the structure of beams and prevent it from damage and corrode for the safety in the future.

将实验中三种驱动器搭配加速度计实验分析结果较佳的一组方法，来从事结构之非破坏检测，探讨当固定梁有缺陷产生时，其结构之自然频率与模态振型的变化，并比较其不同缺陷处之固定梁实验模态结果，将来可应用於梁结构物损坏或腐蚀时侦测其安全性之分析。

And the preparing method has the steps of: fixing the charge transfer complex on a scanning tunnel microscope, starting up the cut mode of the scanning tunnel, controlling the motion of pointer of the scanning tunnel, applying a voltage pulse between the charge transfer complex and the pointer, writing the information hole array in the charge transfer complex.

按如下过程进行制备：将电荷转移复合物固定于扫描隧道显微镜上，启动扫描隧道显微镜的刻写模式，控制扫描隧道显微镜的针尖的运动，在电荷转移复合物和针尖之间施加电压脉冲，在电荷转移复合物上写入信息孔阵。

To study the surface treatment of the quartz glass, and the coupling of the albumin protein, we used with the fictionalization of the copolymers, and experimental conditions for protein immobilization.

蛋白质固定化之制作程序系将白蛋白固定於涂有光阻剂共聚物之石英玻璃表面，然后检测蛋白质於280 nm波长附近的吸光特性，以评估蛋白质固定化的效果。

The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

通过研究阐明：紫外聚合产物TFMAA-co-TRIM中的νO-H振动吸收峰在聚合16h后红移，νC=O振动吸收峰在聚合24h后红移；TFMAA-co-TRIM对雌二醇异构体的印迹因子达到了3.01，α为2.28，优于其它功能单体参与得到的聚合物识别特性；TFMAA-co-TRIM聚合物粒径介于300 nm至1.5μm之间，作为色谱固定相具有良好的通量和低的柱压；在乙腈流动相中，TFMAA-co-TRIM分子印迹固定相的分离过程主要被焓驱动，低温有利于分子印迹固定相分离甾醇结构类似物；TFMAA-co-TRIM分子印迹聚合物在255.84℃时开始熔融，Tp=257.40℃，聚合物CP的降解温度在267.79℃，MIP降解温度在343.11℃，制备的固相微萃取头初步经GC/MS 270℃的耐热性测定；不同的洗提溶剂筛选证明了选择接近聚合溶剂的洗脱体系更有利于MISPE发挥识别效应；分子印迹固相萃取柱对孕酮、17α-雌二醇、17β-雌二醇和雄烯二酮都具有不同程度的保留特性，可作为此类化合物的吸附材料，特别对孕酮强保留的富集特性，可作为孕酮的选择性识别吸附剂，其分子印迹聚合物对17β-雌二醇的吸附动力学测定显示在50 min内基本达到吸附平衡，具有作为传感器核心敏感材料的潜力；对比萃取奶粉中17β-雌二醇性能，MISPE柱比非分子印迹固相萃取柱和C18柱具有更高的保留，回收率依次为85.5%，43.7%和30.7%。

Immobilizing process of catalase on sodium periodate oxidized cotton fabric was carried out, optimum conditions were defined.

以高碘酸钠氧化棉织物为载体固定过氧化氢酶，研究其固定化的主要条件，考察了固定化酶的性质。

Part II:The electrocatalytic oxidation of guanine, adenine, guanosine-5'-monophosphate and ssDNA was performed in the presence of iron bis(2,2':6',2"-terpyridine) and Fe tris(1,10-phenanthroline) complexes as a homogeneous catalysts with Fe redox couple. The electrocatalytic and electroanalytic properties of guanine with an iron bis(2,2':6',2"-terpyridine) complex was measured by the amperometry method using the rotating disk electrodes. Electropolymerization of iron tris(5-amino-1,10-phenanthroline) complex produced thin polymer films on a gold, platinum, nickel, and glassy carbon electrode.

第二部分利用iron bis(2，2'：6'，2&-terpyridine)和Fetris(1，10-phenanthroline)之金属错合物当作均相的催化剂对於鸟嘌呤、腺嘌呤、5'-鸟嘌呤核苷单磷酸盐(guanosine-5'-monophosphate)和单股DNA进行电催化氧化的研究。iron bis(2，2'：6'，2&-terpyridine)之金属错合物对於鸟嘌呤的电催化和电分析性质可利用计时安培法并使用旋转环-碟电极进行测量。rontris(5-amino-1，10-phenanthroline)之金属错合物可经由电聚合过程所形成的聚合薄膜固定在黄金、白金、镍和玻璃碳电极上。

Solves this symptom method to divide into the surgery and the non-surgery treats, the non-surgery treats for example the physical property treatment and the pharmacological treatment, the effect is not often good, the surgery treats may divide into the traditional surgery and micro creates the surgery two kinds, the traditional surgery method is in the lumbar vertebra fusion the pexia, although this method excised has created the lumbar vertebra narrow yellow ligament and the small surface joint, but the surgery time was long, the wound was big as well as although the lumbar vertebra fusion and fixed has removed the pathological change stage abnormal movement, but the neighbor stage's abnormal movement increased, between the awl in plate's pressure elevated obviously, the small articular process exceptionally drew back changes with the fixed stage bone quantityThe loss, causes the fixed stage vertical motion stage to draw back changes ASD[1], but new micro creates the surgery is opens a small wound in the back, and implants the interspinal to open, then solution lumbar vertebra narrow sickness question.

解决此种症状的方法分为手术及非手术治疗，非手术治疗例如物理性治疗和药物治疗，往往效果不佳，手术治疗又可分为传统手术和微创手术二种，传统手术方法就是腰椎融合内固定术，虽然此种方法切除了造成腰椎狭窄的黄韧带和小面关节，但手术时间长，伤口大以及腰椎的融合和固定虽去除了病变节段的异常活动，但邻近节段的异常活动增加，锥间盘内的压力明显升高，小关节突的异常退变和固定节段的骨量丢失，导致固定节段上下运动节段的退变ASD[1]，而新型的微创手术是在背部开一个小伤口，并植入棘突间撑开器，进而解决腰椎狭窄症的问题。

Novozym 435 was used for the synthesis of vitamin A plamitate. The influences of solvent, the molar ratio of substrates, the reaction temperature and time, and the water concentration were optimized and the best result was obtained by interesterification from 0.200 g vitamin A acetate and 0.468 g palmitic acid, at 30℃, in 10 mL hexane, containing 0% of water, with 10% of lipase(mass ratio, immobilized lipase to vitamin A plamitate). In these conditions, 75% of vitamin A acetate was converted into vitamin A plamitate. The immobilized lipase was reused about 6 batches.

对催化合成维生素A棕榈酸酯反应的脂肪酶和反应介质比较，同时对影响合成维生素A棕榈酸酯反应的因素（温度、初始水含量、底物摩尔比、反应时间和酶量等）进行了探讨，优化了反应条件：在10 mL不含水分的正己烷中，0.200 g 维生素A醋酸酯和0.468 g棕榈酸在酶量为10％（指固定化酶与维生素A醋酸酯的质量比）的固定化脂肪酶催化下，在 30℃、190 r/min下反应6 h，转化率可以达到75%，固定化酶可连续使用6次以上。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。