固定化

The experimental process was as follows: a set of 5 immobilized copper ion affinity membranes were loaded into a column, uncombined copper ion was removed with 5 mL of sodium phosphate-citric acid buffer (pH 7); the Hb sample volume was 2 mL, and uncombined Hb was eluted with 10 mL of the buffer.

中文内容：摘要：将纤维素滤纸进行碱处理及环氧活化、偶联亚氨基二乙酸、固定化铜离子等处理，并将其装入自制的色谱柱管，制得固定化铜离子亲和膜色谱柱。

The methods of immobilization,the carriers and the size of carrier which were used to immobilize white rot fungus were studied by comparing removal rate of COD and phenol.

研究了不同的固定化方式、吸附载体及载体体积对吸附法固定化白腐真菌去除苯酚和COD的影响。

Significant loss of GEMs was not observed but strong growth activities, formation of biofilm and zoogloea on the surface of immobile particles were observed via scanning electron microscope. The mechanical stability of the particles remained unchanged after nearly two-month operation.

在整个反应过程中，没有出现大量工程菌流失的现象，同时在固定化颗粒的表面以及浅层均观察到了大量工程菌菌体，固定化颗粒的表面还出现了生物膜和菌胶团，反应结束时，颗粒形态完好，强度满足本工艺条件下长期使用的需求。

According to these reaction conditions, a low aqueous organic biphase system for the continuous production of Naproxen was developed.

反应在一个具有回路的连续流搅拌反应器中进行，反应器中添加有采用吸附法固定化的脂肪酶，载体为一种弱极性的合成载体，水相连同固定化酶颗粒一起永久保持在反应器中，有机流动相带入底物，带出产物。

This paper investigates the conditions of immobilization of chitosan adopting sodium alginate,poly and glutin as carriers,and farther uses the immobilized chitosan to adsorb the Cuand Crin water.

采用海藻酸钠、聚乙烯醇、明胶作为载体，对壳聚糖的固定化条件进行了研究，并以制成的固定化壳聚糖吸附水中Cu2+和Cr6+。

Subcordiformis in Huanghai Sea near Dalian, it can be able to evolve H〓 photosynthetically. Comparing with Chlamydomonas reinhardtii in our lab, the H〓 productivity is same. By the elementary macro regulation of H〓 production with 2, 5-dibromo-3-methyl-6-isopropyl-p-benzoquinone , N, N'-dicyclohexylcarbodiimide , 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea , sodium dithionate, sulfur-deprivation and cell immobilization, the results show that DCCD, immobilization and sulfur-deprivation has a positive effects on the H〓 production.

利用DCCD（质子泵抑制剂N，N'-dicyclohexylcarbodiimide）、DBMIB（光合电子传递抑制剂2，5-dibromo-3-methyl-6-isopropyl-p-benzoquinone）、DCMU[PSⅡ光合放氧抑制剂3-（3，4-dichlorophenyl）-1，1-dimethylurea]、连二亚硫酸钠、无硫调控及固定化扁藻等手段，对该扁藻产氢进行初步的宏观调控，发现DCCD的加入、扁藻的固定化和无硫调控对产氢均有正效应，其中无硫调控使扁藻产氢时间延长，总产氢量提高了近10倍。

Chitosan alginate loaden enzyme have prominent effect for activity of immobilized enzyme by orthogonal experimental design, and getting optimum condition .

经正交实验L_8(2~7)，壳聚糖、海藻酸钠、载酶量对固定化酶活力影响比较显著，确定最佳固定化酶条件。

As known as traditional viewpoint, microbe could produce hydrogen continuously only when microbic cell is fixed, so present tests of this process offen adopt this process fixed-cell technology of pure microbe and were in the scale of batch fermentation test. Now the larggest ability of bio-producing hydrogen was 20mLH〓/g biomass·h produced by Rhodospirillum rubrum and 1. 6mLH〓/g biomass·h produced by Clostridium butyricum .

传统观点认为，微生物惟有进行细胞固定化才能达到持续产氢，所以现有试验常采用纯种细胞固定化技术，且尚处于分批培养试验阶段，最大产氢能力深红螺菌为20mLH〓/g菌体·h，丁酸梭状芽孢杆菌为1.6mLH〓/g菌体·h。

In addition, under the above-selected nitrosification condition, the immobilized cells N4 and B08 were used for the combined short-cut biodenitriflcation with the ratio of immobilized cells 1:1 and the seed volume 15%, 27h the denitrogenation efficiency reached 97.53%; the immobilized cells N4 and B08 were used to do denitrogenation application test on eutrophic lake water of our school, 12h the NH4+-N removal rate was 84.06%.

在以上所选亚硝化条件下，按1：1的比例，亚硝化细菌N_4和反硝化细菌B_(08)固定化细胞接种量为15％，广西大学硕士学位论文固定化微生物捷径生物脱氮的研究作联合捷径脱氮试验，27h脱氮率为97.53%；并作本校富营养化湖水的脱氮应用试验，处理12h，NH4十一N去除率为84.06%。

The results showed that the optimum tempreture and thermostable temperature of immobilized enzyme were all 65 ℃, which was 5 ℃ higher than that of the MTHase.

结果显示：固定化酶的最适反应温度和稳定温度都由原酶的60 ℃提高到65 ℃；固定化酶的最适反应pH值较原酶的5.5降低到5.0。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。