固定化

Simulated Annealing with a constant factor temperature reduction scheme and fixed numbers of inner and outer iterations is chosen.

用～模拟韧化内部的和外部的重复不变的因素温度减少方案和固定的数字被选择。

In the thesis alkylate and bridging resorcarenes were used as CGC SP. Their chromatographic character were studied.

将烷基化和桥联的间苯二酚杯芳烃用作气相色谱固定相，考察了色谱性能，对普通化合物表现出良好的分离能力，尤其是对芳香族位置异构体有良好的分离作用。

The invention aims at improving the quality of flour to researche a method for preparing new flour modifying agent, namely, the microencapsulation glucose oxidase, chitose-calcium alginate-glucose oxidase microcapsule which is obtained through the method is copolymer hydrogel which is obtained through double cross-linking, both amidogen and carboxyl group are fixed, and the whole system is a reticular formation.

本发明以改善面粉品质为目的，研究一种新型面粉改良剂――微胶囊化葡萄糖氧化酶的制备方法，采用上述方法制得的壳聚糖-海藻酸钙-葡萄糖氧化酶微胶囊，是经双重交联得到的共聚凝胶，氨基和羧基均被固定，整个体系为网状结构。

But because the proportion was small, the part of the fixed ammonium could be ignored.

矿化量中有一小部分来自固定态铵的释放，但由于所占比例较小可以忽略不计。

In the end, the bioactivity and biocompatibility of samples were studied based on apatite precipitation and osteoblast culture in vitro.

最后，通过体外仿生矿化和成骨细胞培养来研究固定蛋白前后样品的表面生物活性和生物相容性。

Methods: MII, GV and IVM-MII were vitrified. After thawed, they underwent in vitro maturation, in vitro fertilization, embryo cultivation or fixed for immunofluorescence. The rates of survival, maturation, fertilization, blastulation and cytoskeleton with normal configuration were calculated.

玻璃化冷冻生殖泡期卵、成熟中期卵及体外成熟卵，解冻复苏后，分别作体外成熟、体外受精、胚胎培养或固定作免疫荧光标记，统计复苏率、成熟率、受精率、囊胚率、细胞骨架正常率。

ABSTRACT Purpose By adding additives in carbonated hydroxyapatite cement to increase its rheology to prepare injectable carbonated hydroxyapatite cement.(1) Test the physicochemistry characteristics of ICHA cement.(2) Evaluate the biocompatibility of ICHA cement.(3) Biomechanieal appreciation of ICHA cement used in distal radius fracture.(4) Study the mechanical stability, degradation and bone formation ability of ICHA cement used in goat lateral tibial plateau compression fracture.

目的 在碳酸化羟基磷灰石(Carbonated hydroxyapatite，CHA)水泥原位固化基础上进行改性研究，通过改变其流变学特性，达到具有良好注射性能的可注射性碳酸化羟基磷灰石(Injectable carbonated hydroxyapatite，ICHA)水泥，为骨科的微创治疗提供新方法：(1)制备ICHA水泥，完成理化性质的检测；(2)进行ICHA水泥的生物相容性评价；(3)探讨ICHA水泥在桡骨远端骨折应用的生物力学固定强度；(4)研究ICHA水泥在实验性大动物胫骨平台压缩性骨折中应用的力学稳定性、降解性能及成骨性能。

Winding: winding on the φ 5mm bakelite bar with the winding machine (2) temperature seasoning: 48 hour in silicon oil, 1 hour in LN2,(3) coil sealing: polyester,(4) pressure seasoning: keeping 30 minutes in 8000 10000kg cm2,(5) firm fix of the pressure gauge.

线圈之缠绕：利用绕线机缠绕 5mm 电木棒，(2)温度季化： 48 小时於矽油中， 1 小时於液态氮中，(3)线圈封装：使用香乳胶封装线圈，(4)压力季化：加压一次 30 分钟到 8000 10000kg cm2 ，(5)固定压力计。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

In our research, through derivatization of stable calix[4]pyrroles, active substituting units were jointed on the calixpyrrolic skelecton. After acylamide and free radical transfer reaction, the preparation of new calix[4]pyrrole-bonded stationary phases were fulfilled. the separation abilities of them to inorganic and organic anions and medicines were exploited and new analytical methods were established. The separation mechanisms were discussed. At the same time, through using calix[4]pyrroles as additives in CZE to influence the electrophoresis of inorganic anions, the recognition mechanisms and influencing factors in supramolecular chemistry were also studied.

本项目以新型杯吡咯大环化合物分子识别化学研究为基础，设计合成了含活泼取代基的官能化杯[4]吡咯化合物，通过酰胺化反应和原位自由基链转移反应制备了新型的杯[4]吡咯色谱固定相，系统研究了具有阴离子识别功能的杯[4]吡咯大环化合物作为离子色谱的分离介质对无机、有机离子型化合物和药物分子的色谱分离性能，建立了新的分析方法，对分离机理进行了探索和验证；同时将杯[4]吡咯化合物用作毛细管电泳流动相添加剂，利用其对无机阴离子电泳行为的影响探讨超分子化学中分子识别的机理和影响因素。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。