固定化

Flow injection fluorometry for the determination of carbamazepine in pharmaceuticals and plasmaA simple, sensitive and specific spectrofluorimetric method has been developed for the determination of CBZ. The method was based on lead dioxide solid-phase reactor flow injection on-line oxidizing CBZ into fluorescent product in medium of phosphoric acid. The product has green-yellow fluorescence at excitation wavelength of 355 nm and emission wavelength of 478 nm, and the fluorescence intensity could be enhanced in the presence of acetone. The reaction conditions and experimental conditions were studied and optimized.

二、流动注射在线氧化荧光法测定卡马西平的研究利用卡马西平自身无荧光，经过氧化后可转变为在最大激发波长为355nm，最大发射波长为478nm的强荧光物质，而且丙酮对该荧光体系有增敏作用的现象，应用流动注射体系建立了以醋酸纤维素固定化的二氧化铅作为固相反应器、在磷酸介质中在线氧化荧光法测定卡马西平的新方法。

The results indicate that sepoilte heat-treated in air at 400℃ is the best immobilized carrier of the strain.

结果表明，活化温度为400℃的海泡石最适宜作固定化载体。

Could be used as an efficient biosorbent for removal of heavy metal ions from aqueous solution and that the process was strongly affected by pH.

研究发现，溶液pH值对吸附过程有较大影响，最佳吸附pH值在5.5附近；最佳吸附温度为25～35℃；溶液浓度在10～500mg·L^-1范围内，固定化简青霉菌对重金属的吸附随金属离子浓度的增加而增加；吸附过程符合Langmuir等温吸附模型；生物吸附平衡时间约为60min。

Coli. The protein was expressed in a soluble form and as a fusion protein. There was a 6 histidines tag at its amino acid terminus. It was purified by immobilized metal ion affinity chromatography under native conditions.

所表达的蛋白是一种可溶性的融合蛋白，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。

This IGAD can be applied for GABA biotransformation continuously.

该固定化谷氨酸脱羧酶可应用于生物转化法连续制备GABA。

When reacted four times, half of the IGAD lost activity. The average conversion of glutamate by IGAD was above 90%.

固定化酶的最适pH为5.0、最适温度为42℃、最适底物浓度1%，反应进行4次后一半的酶活损失，底物转化率在90%以上。

IGAD by sodium alginate can produce γ-aminobutyric acid well which may possibly be used as an industrial method to produce GABA.

海藻酸钠法固定化谷氨酸脱羧酶可以很好的转化谷氨酸钠生成γ-氨基丁酸，具有实用的潜力。

Results 65.3 % enzyme activity of IGAD could be reserved compared with unimmobilized one when the concentration of sodium alginate, gelatin, CaCl2, glutaraldehyde and solidifying time were 2.5%, 1.0%, 3.0%, 0.3% and 4 h respectively.

结果 海藻酸钠、明胶、氯化钙和戊二醛浓度分别为2.5%，1.0%，3.0%和0.3%，硬化时间4h，固定化酶的相对活性最高，酶活回收率达到65.3%。

Purpose:The aim is to study the optimal co reaction condition of producing high content fructooligosaccharides for immobilized cells and glucose isomerase.

目的 ：研究固定化黑曲霉细胞与葡萄糖异构酶协同反应生产高含量低聚果糖的优化条件。

It has been indicated that antibiotic activity of lincomycin reaction solution increased twofold in after 32h reaction with immobilized protoplast of S.

用固定化金霉素产生菌（金霉素链霉菌，S.aureofaciens）的原生质体，在适当量的氯离子存在时，转化林可霉素生成抗菌活力比其本体高的产物。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。