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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在囊胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Great Staff, G Clef Staff, F Clef Staff, with note name, with Lyrics

伟大工作人员地下欧洲妇女游说的工作人员,女工作人员的欧洲妇女游说,同注姓名,与歌词)' 5 ^#?

Skylights, clerestory windows, and glass block are all options that maintain privacy.

购: 红星美凯龙年末购:全场3折起,进店有礼!

The wavelet coefficient energy group of time-frequency surface has distinct difference for different swept extent.

储层的孔隙度和电阻率曲线间微差形态波形的复杂程度隐含着储层含油气性的信息,油层水淹程度不同,其时频平面上小波系数能量存在着显著差异,随水淹程度加重,其能量分布由复杂逐渐趋于简单单调。

In the one half part, according to the strontium nature, thermodynamic calculation of correlative reaction and the principle of the vacuum aluminothermy reduction process, with the laboratory findings of preparation strontium by vacuum aluminothermy reduction compared and analyzed in different parameter conditions, a variety of factors which effect the decomposition rate of SrCO_3 and the percent reduction of strontia are gotten out. The factors include that the decomposition rate of SrCO_3 is influenced by different addition and pressure. They also include that the percent reduction of strontia is influenced by excess coefficient of reducing agent aluminium powder, the particle size of raw material or reducing agent, the pressure of barbecuing, temperature and reduction time. According to those, we can draw the conclusion as follows:(1) Decomposition temperature of SrCO_3 is decreased by adding carbon and alumina in different degree.(2) With quantitative carbon added under the vacuum condition, the decomposition temperature of SrCO_3 is decreased obviously, which can fall 150℃ compared with atmospheric pressure.(3) In keeping 1150℃ of 2.5h, under the 5Pa vacuum condition, the decomposition rate of SrCO_3 nearly keeps in 99% steadily.

在前半部分的真空铝热还原法中,根据锶的性质、相关反应的热力学计算及真空铝热还原法原理,通过对不同参数条件下的真空铝热还原法生产金属锶的实验结果的分析比较,得出了影响SrCO_3的分解率和氧化锶的还原率的种种因素,具体因素包括不同添加剂、不同气压对SrCO_3分解率的影响,还原剂铝粉的过量系数、原料与还原剂的粒度、制压力、温度和还原时间等对氧化锶还原率的影响,得出具体结论如下:(1)添加碳和Al_2O_3能不同程度的降低SrCO_3的分解温度;(2)真空条件下加入一定量的碳可显著降低SrCO_3的分解温度,比之常压下可降低150℃之多;(3)在1150℃恒温2.5h、真空度达到5Pa的条件下,SrCO_3的分解率几乎保持稳定在99%;(4)氧化锶的还原率随还原剂过量系数的增大而增大,但是趋势越来越小,当过量系数超过25%后,氧化锶的还原率几乎不再增加;(5)氧化锶的还原率随原料与还原剂的粒度的变细而增加;(6)氧化锶的还原率随制压力的增加而增加,但超过一定值后,氧化锶的还原率反而会下降;(7)氧化锶的还原率随还原温度的升高而增加;(8)氧化锶的还原率随还原时间延长而增加,在本实验条件下,超过2.5h趋于稳定。

The maximum clique problem is a classical combinatorial optimization problem, and finds many important applications in different domains, this paper surveys the current progress on MCP. The Cross Entropy method for combinatorial optimization is proposed by Reuven Y.

最大问题是一个经典的组合优化问题,在理论研究和实际应用方面都有重要的价值,本文对最大问题的最新研究进展做了分析综述。

An application made by a person authorized by a body corporate not possessing a common seal shall include a declaration by that person that the body concerned does not possess a common seal .

由并无法印章的法人体所授权的人提出的申请,须包括一份由该人所作的声明书,声明该法人体并无法印章。

The maximal hyperclique pattern is a more compact representation of a group of hyperclique patterns, which is desirable for many applications.

极大超模式是一组超模式更加紧凑的表示,可被用于多种应用。

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