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The experimental group received 10 weeks of walking exercise training, at 50 minuets per session, 5 days per week and at a exercise intensity limit between 60% and 70% maximum heart rate. The control group received ordinary special education curriculums. All participants completed three times of the PACER(Progressive Aerobic Cardiovascular Endurance Run)20-Meter Shuttle Run test and test for the average walking speed during 1600 meters:before the experiment started , after the experiment and the follow-up test at the fourteenth week of the experiment started. All results from these tests were recorded and analyzed by descriptive statistics; repeated measure t-test; the one way analysis of covariance .

实验组进行星期一至星期五每天一次,合计每周五次,每次50分钟,总共10周,运动强度为60%-70%最大心跳率的步行运动;控制组接受学校安排的特殊教育课程,两组学生在进行步行运动前、后以及实验处理结束后第四周,分别接受PACER渐进性心肺耐力跑-20 公尺和1600公尺的步行速度之测验,并将所得的成绩以描述性统计、重复量数t 考验、单因子共变数分析进行统计考验,评估实验处理之运动与维持成效。

Methods: rabbit bone marrow mesenchymal stem cells were purified, and then cultured in inducing medium containing transforming growth factor-β1; mtt assay was employed to evaluate the proliferative activity of induced cells; immunohistochemical assay was used to detect the expression of type ⅱ collagen; induced cells were transplanted into the knee of rabbit, and x-ray photography was employed to observe the repair and formation of cartilage.

纯化兔骨髓间充质干细胞;用含转化生长因子β1的诱导液培养骨髓间充质干细胞;以mtt测定诱导细胞的增殖活性;免疫组化检测ⅱ型胶原蛋白的表达;诱导后的细胞移植于白兔膝关节内,x线摄片观察软骨修复形成情况。

When comparing quitters with persistent smokers, quitting smoking was the most important survival factor, reducing patients' mortality risk by 40%.

戒烟者与未戒烟组相比,戒烟是最重要的生存因子,使病人的死亡率下降近40%。

Methods Immunohistochemistry combined with computer-assisted image analysis system were carried out to examine the expressions of factor Ⅷ-related antigen,bFGF and PCNA in 18 normal prostates and 62 specimens of BPH.

应用免疫组化结合计算机图像分析方法检测第Ⅷ因子相关抗原、bFGF和PCNA在18例正常前列腺和62例BPH中的表达及其相关关系。

None of the subjects had any classic risk factors for cardiovascular disease, and levels of risk markers (such as serum lipids, plasma glucose, and body mass index) were similar in the cases and controls.

研究对象中没有一个有典型的心血管疾病危险因子,一些危险标志物水平(例如血清脂类,血浆葡萄糖及体重指数)病例组与对照组无差别。

objective to investigate the relation between psychological factors and functional dyspepsia.methods scl-90was performed in50patients with fd and30health controls.results there were significant difˉference in somatic,interpersonal sensitive,anxious,depressive,phobic,hostile and paranoic scores of scl-90between two groups(p.05).somatization,interpersonal sensitivity,anxiety and depression were positively associated with the severity of fd.conclusion there were varied psychological problems in fd patients,and the treatment should be individualized.

目的 探讨功能性消化不良和心理因素之间的关系。方法采用症状自评量表(scl-90)对连续就诊的50例符合罗马ⅱ标准的功能性消化不良患者及30例匹配的正常对照进行测评。结果 fd组的躯体化、人际关系紧张、焦虑、抑郁、强迫、恐怖、敌对和偏执因子分均高于正常对照组,其中焦虑、人际敏感、躯体化、抑郁与fd的严重程度成正相关。结论功能性消化不良存在不同程度的心理障碍。

METHODS Dinitrofluorobenzene was used to induce the contact dermatitis in mouse ear, rhIL-11 0.375-1.5 mgkg^(-1)d^(-1) sc for 10 d, the degree of skin inflammatory reaction was observed. At the same time, the serum level of tumor necrosis factor-α and interleukin-6(IL-6) was measured by radioimmunoassay; the serum level of nitrogen monoxidum was detected by biochemical method and the expression of intercellular adhesion molecule-1(ICAM-1) was detected by immunohistochemical method.

采用2,4-二硝基氟苯致小鼠耳部皮肤接触性皮炎试验模型,观察sc rhIL-11 0.375~1.5mgkg^(-1)d^(-1),连续10d后皮肤炎症反应程度,同时采用放射免疫分析法测定血清中肿瘤坏死因子-α和白细胞介素-6(IL-6)的水平,采用生物化学检测法测定血清一氧化氮的水平,采用免疫组化法测定皮肤中细胞间粘附分子(ICAM-1)的表达。

Crohn's disease was induced by trinitrobenzene sulfonic acid in the model group. The mice were sacrificed 5 days afterwards and LPMC were isolated and cultured. The expression of CCR2 and CCR5 on LPMC were determined by flow cytometry. The secretion of MIP-1α in the supernatant was determined by enzyme-linked immunosorbent assay, and the expression of MIP-1α mRNA was determined by reverse transcriptase polymerase chain reaction.

模型组小鼠以三硝基苯磺酸灌肠造模。5d后处死,提取和培养LPMC,采用流式细胞仪测定小鼠LPMC中趋化因子受体CCR2、CCR5的表达;采用酶联免疫吸附测定检测培养上清液中MIP-1α的分泌;采用逆转录聚合酶链反应检测MIP-1α mRNA的表达。

Methods SCL 90 table was applied to evaluate the psychological health status of the coalminers pneumoconiosis patients; depression and anxiety syndrome related factors were found out; analysis was made on the result compared with that of the control group and the national norm with SPSS software.

通过采用症状自测量表(SCL-90)测评煤工尘肺患者的心理健康状况,从中找出抑郁、焦虑组症候群因子,利用SPSS软件,与对照组及全国常模对比分析。

The IgA nephropathy rats model were made by the method of oral BSA and intravenous SEB, the mRNA and protein expression of TGF-β1 was measured by in-situ hybridisation and immunohistochemistry.

采用口服牛血清白蛋白及静注葡萄糖球菌肠毒素制成小鼠IgA肾病模型,免疫组化和原位杂交检测肾小球转化生长因子β1(TGF-β1)蛋白和mRNA。

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此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

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