因子组

Our data revealed glucose and beef extract to be the most influential parameters, and the composition of the optimal fermentation medium (100 mL) was determined to consist of 4.46 g glucose, 2 g soluble starch, 4 g silkworm powder, 2.93 g beef extract, 0.10 g KH2PO4 and 0.15 g MgSO4·7H2O.

最后通过中心组合设计和响应面分析法，确定了主要影响因子的浓度。优化后的培养基组成为：葡萄糖4.46 g/100 mL，可溶性淀粉2 g/100 mL，蚕蛹粉4 g/100 mL，牛肉膏2.93 g/100 mL，KH2PO4 0.1 g/100 mL和MgSO4·7H2O 0.15 g/100 mL。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

Semi-quantitative RT-PCR analysis showed that ZF1 expressed in root, stem, leaf, flower, immature pod, and embryo of chickpea with different expression patterns.

半定量RT-PCR分析表明，ZF1在鹰嘴豆的根、茎、叶、花、幼荚和幼胚中均有表达，在茎和叶中表达较弱，为组成型转录因子。

The changes of MDA in the plasma, CINC/IL-8 and NO in the serum, MPO in the skeletal muscle were studied at the end of ischemia and 1h, 2h, 4h, 8h, 12h during reperfusion.

分别于缺血4h末，再灌注1、2、4、8、12h，测定各组血浆中的MDA、骨骼肌组织中的MPO，血清嗜中性粒细胞化学趋化因子(CINC／IL-8)，血清一氧化氮，用流式细胞仪及免疫组化法观察各组白细胞上粘附分子CD11b／CD18和骨骼肌血管中ICAM-1的表达情况及各组的组织学改变。

The control group received same volume of normal saline instead. The platelet, prothrombin time, activated partial thromboplastin time, fibrinogen, factor Ⅷ coagulator activity, the operation duration, and blood loss within postoperative 24 h were determined and compared between 2 groups.

测定两组用药前和术后的血小板计数、凝血酶原时间、部分凝血活酶时间、纤维蛋白原定量、第八因子促凝活性；记录手术时间和手术开始至24 h中出血量，评价其临床效果。

Solid-phase enzyme discovery and applications in biochemistry, microbiology, medicine, chemical engineering and other areas of great significance, such as the simulated bio-vivo multi-enzyme system, and will complete a group response to a variety of enzymes and secondary factor immobilized can be made into a particular reactor.

固相酶的发现和应用在生物化学、微生物学、医学、化学工程等领域有着重要的意义，如模拟生物体内的多酶体系，将完成某一组反应的多种酶和辅助因子固定化，可以制成特定的反应器。

In the present study, Hartree-Fock and Density Functional Theory with different basis sets were employed to calculate the structural and thermodynamic parameters of 66 thiophenyl, phenyl sulfoxide and phenylsulfonyl cycloalkane carboxylates. With the statistic software SPSS11.5 for windows and GQSARF2.0, the QSAR models relating physicochemical properties of these compounds to their structural and thermodynamic parameters were successfully obtained, using multiple stepwise regression method. The physicochemical properties observed in this work include octanol/water partition coefficient, chromatographic capacity index, toxicity (EC50 and LC50), and solubility etc.

本文采用从头算方法中的Hartree-Fock 方法、密度泛函理论方法(Density functional theory，简称DFT)中的不同基组，计算了苯硫基、苯亚砜基和苯砜基含硫芳香羧酸酯三类共66 个化合物的结构参数和热力学参数，利用SPSS11.5 for windows 统计软件和GQSARF2.0 软件，采用多元逐步回归法拟合得到与溶质-溶剂相互作用有关的物理化学性质与结构参数和热力学参数之间的QSAR 方程，这些物理化学性质包括辛醇/水分配系数、色谱容量因子、毒性(EC50和LC50)、溶解度等。

The pretreatment of inhibitor was divided into two groups: discard pretreatment liquid group and reserve pretreatment liquid group. At the same time we also tested the activity of caspase3 when cell was pretreated by VerapamiK YTNF3 or TNF-3 . The results indicated that the blocking effect on Y TNF P or TNF P induced L929 cytolysis was significantly lower in inhibitor pretreatment discarding group than in pretreatment reserved group.

为了探讨细胞因子与受体结合后的信号转导速度及抑制剂发挥作用公的时间效应，我们进行了抑制剂预处理和 Y hF i及 iiiF 6预处理实验，二其中抑制剂预处理又分为抑制剂去除和保留两组。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。