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Folds and 5. 3 folds after culturing 1 and 2 Weeks. There is no CFC after 3 weeks in culture.

有滋养层加细胞因子组,在培养了1周、2周和3周后,CFC的扩增倍数分别为25倍、17.7倍和14.3倍。

METHODS: The right peroneal nerve was transected and an epineural window of 1 mm was created on the neighboring tibial nerve.

表皮生长因子组:切断右侧腓神经,在邻近的胫神经干外膜上开一1 mm小窗,将腓神经远端吻合到胫神经干侧方开窗处。

Methods: The bilateral superior buccal branches of facial nerve were exposed and transected, a nerve growth chamber were created by suturing the epineurium and silicone chamber under microscope .The chambers of experimental sides were injected with HGF/normal saline and that of the control sides with normal saline of same amount (10μL ),the regenerated nerves in the chambers were dissected and postoperative evaluation were performed byelectrophyiological testing , histopathologic, morphological studies under gross observation, light microscope at 1,2,4 weeks after operation, respectively.

实验设生理盐水对照组和肝细胞生长因子组,选用30只成年、健康新西兰家兔,暴露并分离双侧面神经上颊支,将其横断后套入硅胶管内,用显微外科技术将硅胶管与神经外膜缝合以形成硅胶神经再生腔,在一侧硅胶神经再生腔内注入HGF为实验侧,在另一侧注入生理盐水为对照侧,于术后1周、2周、4周分别麻醉家兔后行电生理检测,在肉眼、光镜、特殊染色下进行组织形态学观察,并在光镜下进行形态定量分析。

Methods Whole blood sample (2ml) acquired from 12 volunteers via a large antecubital vein was heparinized with 10U/ml heparin sodium and ice-bathed down to 4℃ and divided into the following groups: control group , PAF group , FMLP group, PAF+FMLP group , FMLP+lidocaine (10-4 M) group and lidocaine (10-4M,10-5M,10-6M )groups . the expression of CD11b in neutrophils was detected by flow cytometry in different groups .

选12名健康的成年志愿者,每位志愿者抽肘前静脉血2ml,肝素抗凝,置冰浴中降温至4℃分组如下:对照组,血小板活化因子组,趋化寡肽组,血小板活化因子+趋化寡肽, FMLP+利多卡因(10-4 M)组和P+F+利多卡因(10-4M,10-5M和10-6M)组,分别测定PMN CD11b的表达水平。

Objective To study the mechanism in which the personality, copying style influenced survival time by changing immunity function in the patients with gastric cancer Methods The Minnesota Multiphase Personality Inventory, coping style questionnaires and immunity function were measured in patients with gastric cancer on late stage, gastric cancer patients with longer survival time, and normal control Results Psychopathic deviate, social introversion subscale scores of MMPI in groupⅡ were significantly lower than that in the other two groups ( P 05) Statistical difference were found between groupⅠand groupⅡ in problem saving factor and help seeking factor of CSQ ( P 05) Natural killer cell level in groupⅡ was significantly higher than in groupⅠ( P 05) There was negative correlation between the NKC and depression, psychopathic deviate, paranoia, psychasthenia, schizophrenia subscale scores of MMPI, there was positive correlation between NKC and SP, coping type in patients with gastric cancer Conclusion The gastric cancer patients with longer survival time are more extroversive and have more mature coping styles Their NKC level is relatively higher The results may be advantageous to improve the prognosis of gastric cancer

作者中文名:王健;邹义壮;唐丽丽;李杰;张东;金山;刘娟摘要:目的研究胃癌患者的生存期与个性特征、应付方式等心理因素,以及免疫功能的关系。方法对胃癌晚期患者、胃癌较长生存期患者及正常健康人分别进行明尼苏达多相个性测验、应对方式问卷、免疫功能测试,并做相关分析。结果(1)MMPI:胃癌Ⅱ组人格障碍评分(42.0±10.5)及社会内向评分(42.2±11.6),低于其他两组[胃癌Ⅰ组为(49.3±11.2)分和(48.3±9.4)分,对照组为(48.6±10.9)分和(39.9±11.5)分;P均<0.05];(2)CSQ:解决问题和求助因子分,胃癌Ⅰ组[分别为(0.68±0.27)分和(0.42±0.18)分]均低于胃癌Ⅱ组[分别为(0.82±0.18)分和(0.65±0.21)分;P<0.05和P<0.01],胃癌Ⅱ组更多采取成熟的应对方式;(3)自然杀伤细胞:胃癌Ⅱ组水平(21.4±9.2)高于胃癌Ⅰ组[(14.2±8.8);P<0.05];而NK细胞水平与MMPI的抑郁(r=-0.35)、人格障碍(r=-0.39)、偏执(r=-0.29)、精神衰弱(r=-0.30)及精神分裂(r=-0.30)等量表呈负相关,与CSQ的解决问题因子(r=0.35)及应付方式类型(r=0.34)呈正相关。结论与胃癌Ⅰ组比较,胃癌Ⅱ组更为外向,负性情绪较少,更多采取成熟的应对方式,其NK细胞水平较高,对胃癌的预后产生了积极的影响。

RESULTS: The[99Tcm]-NGF encapsulated in self-made liposomeA presented high radiation in the brain. But[99Tcm] -NGF alone was almost completely excreted through urinary system and[99Tcm] -NGF encapsuled in ordinary liposomeB was mostly phagocytized by liver reticuloendothelial system.

结果:自制注射用脂质体A组包裹的[99Tcm]-神经生长因子经SPECT显像,其在脑部的放射性计数较高,而[99Tcm]-神经生长因子组几乎全部从泌尿系统代谢、[99Tcm]-神经生长因子-普通脂质体B组基本被网状内皮系统吞噬后沉积于肝脏。

Methods The rat MSCs were isolated primarily from the femurs and tibias of the Wistar rats. MSCs were cultured, proliferated and purified by passage culture. After being induced by bFGF, cultuered MSCs were divided into experimental groups (GM1 group, GDNF group and GDNF+ GM1 group) and control group.

取雄性Wistar大鼠股骨和胫骨骨髓,进行MSCs的体外培养、传代扩增及纯化。bFGF预诱导24 h后,依据加入的神经营养因子不同分为单唾液酸四己糖神经节苷脂(GM1)组、胶质源性神经营养因子组和GDNF+GM1组,以及对照组。

Thirty SD rats were randomly divided into five groups: control, 1 week, 2 week, 3 week and 4 week and stimulating the right triceps surae was stimulated by electrical stimulator. Using real-time quantitative PCR measurement to measure two alternative splices of IGF-1(IGF-1Ea and MGF) and MyoG mRNA expressing of gastrocnemius and right biceps brachii.

SD大鼠30只,随机分为5组,每组6只,分别为对照组和1、2、3、4周电刺激组,刺激部位为右侧腓肠肌,实时荧光定量PCR法分别检测双下肢腓肠肌和前肢肱二头肌的胰岛素样生长因子-1(IGF-1)不同变构体肝型胰岛素样生长因子(IGF-1Ea)和机械力生长因子以及成肌分化因子(Myogenin, MyoG)mRNA表达。

Following successful modeling, rats of bFGF group were intratracheally injected with 400 U bFGF and rats of VEGF group with 2 μg VEGF, once a week for three times. MSCs group was injected 1 mL suspension of 4×109/L MSCs into tail vein. MSCs+VEGF group was injected MSCs into tail vein and intratracheally injected VEGF (2 ug, three times) at the same time. Model control and normal control groups were intratracheally injected with equal volume of sodium chloride.

成功造模后,碱性成纤维细胞生长因子组气管内注入400 U碱性成纤维细胞生长因子,血管内皮生长因子组气管内注入2 μg血管内皮生长因子,1次/周,共3次;单纯细胞移植组于尾静脉注入4×109 L-1骨髓间充质干细胞悬液1 mL;血管内皮生长因子+细胞移植组气管内注入血管内皮生长因子的同时,尾静脉注入骨髓间充质干细胞;模型对照组、正常对照组给予相同体积的生理盐水。

Result: Beneficial effects on nerve conduction speed , fiber diameter, fiber amount and thickness of medullary sheath were also observed in the permeable PLA NGCs.

实验结果:术后三个月聚乳酸导管/神经生长因子微囊组在神经传导速度及形态学中的再生纤维直径、纤维数、纤维密度、髓鞘厚度等均优于聚乳酸导管直接吻合组及聚乳酸导管内注入神经生长因子组;和自体神经移植组的结果基本相似。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。