因子

MethodsNeural tubes isolated from the region between midotic placode and third somite of mouse embryo at ED 8.5d were cultured in DMEM/F12 serum-free medium containing basic fibroblast growth factor and epidermal growth factor. The cell cycle of CNCCs was analyzed with flow cytometry, the proliferation capability was determined by MTT method, AP-2α expression was detected by immunocytochemistry as a biological marker of cardiac neural crest cells. The features of migration, multi-directional differentiation were observed as well.

材料与方法成年2月龄C57／BL6小鼠交配，取ED8.5d小鼠胚胎枕中部至第3体节神经管，应用包含碱性纤维生长因子和表皮细胞生长因子的无血清培养基培养，利用MTT法检测细胞增殖能力，利用流式细胞仪检测细胞周期，采用转录激活因子2α(AP-2α，Activator protein-2α)作为其生物学标记物，观察其增殖状况、细胞周期、迁移、分化等生物学特性。

The first stage is screen experiment. A 24-1 Fractional Factorial Design and resolution IV experiment is used to find out which main effect and interaction effect have obvious influence on the precision positioning of feeding table by these four factors. The Minitab program including Half Normal Plot, Pareto Chart, and ANOVA is applied to diagnose obvious effects. The second stage is optimization experiment. The fitness of regression model is obtained by using CCD of RSM with statistical hypothesis testing, regression analysis, lack-of-fit testing, and residual analysis. The optimum values can be found out by using response surface plot and contour plot.

在第一阶段为筛选实验采用24-1部分因子实验，解析度为IV，求出这4个因子主效应及交互作用哪些会对进给平台定位精度有显著的影响，在分析方面使用统计软体Minitab做初步分析，应用了半常态机率图、柏拉图与变异数分析来判断出显著因子与交互效应显著，第二阶段优化实验采用反应曲面法的中央合成设计，搭配统计检定、回归分析、缺适性检定(Lack-of-Fit)、残差分析来取决回归模式之合适性，最后根据反应曲面图及等高线图求出最佳值做验证实验。

In second generation, the result is contrary.4. The results of the two orthogonal designs of artificial diet of rice stem borer both indicate that sucrose and wheat germ flour are key factors of survival days of rice stem borer.

二化螟人工饲料两次正交试验的结果均表明，蔗糖和麦芽粉是影响二化螟幼虫历期的关键因子，水稻茎粉、麦芽粉、酵母粉和稻糠粉是影响二化螟幼虫成活率的关键因子，水稻茎粉是影响二化螟蛹重的关键因子。

We found significant differences in the patterns of cytokines elicited by accination (where IFN- was by far a subdominant response) s natural infection; in addition, there was fairly significant intersubject ariation.

我们发现疫苗接种诱发的细胞因子(其中，IFN-是最强的亚优势应答)和自然感染诱发的细胞因子的模式有显著性差异；此外，还有相当显著的因子间的变异。

If a child had one or more risk factors present in addition to a BMI higher than the 85th percentile, he or she was referred for a fasting blood glucose test and an oral glucose tolerance test. Any child with a BMI higher than the 95th percentile was referred for a fasting blood glucose and a glucose tolerance test, even if no other risk factors were present because a BMI in this percentile is a strong risk factor for prediabetes or diabetes, Dr. Tilson said.

Tilson医师表示，如果该儿童除了BMI高於前85%之外还有一个以上的危险因子，这些儿童被转介接受空腹血糖或是口服葡萄糖测试；如果该儿童BMI高於前95%，则不论是否有危险因子都被转介接受空腹血糖或是口服葡萄糖测试，因为过高的BMI本身就是糖尿病前症或是糖尿病的危险因子。

With these age referenced ranges, a physician in the specialty can also titrate the dose of human growth hormone to reach it optimal level in the patient ( that of a 20 year old healthy Chinese ).

从而我们可以判定这个被检查的病人是健康（胰岛素样生长因子-Ⅰ的水平处于正常范围）、不健康（胰岛素样生长因子-Ⅰ的水平低于其年龄所对应的参考范围），还是可能有肿瘤或疾病（胰岛素样生长因子-Ⅰ的水平值高于其年龄所对应的正常范围最大值）。

The parameter adjust algorithm is also deduced. The canceling methods are discussed when the unideal factor of the device is known or unknown. The result proves that when the unideal factor of devices is unknown, the parameter solved by the iteration is convergent to that when the unideal factor is known.

本文推导了参数调节算法，并讨论了器件不理想因子已知与未知两种情况下的消除方法，证明了在器件不理想因子未知情况下采用迭代方法确定的参数最终收敛于不理想因子已知情况下的参数。

More recent evidence suggests that antimicrobial peptides are effective adjuvants, are synergistic with other immune effectors, initiate the adaptive response, support wound healing, induce or modulate of chemokine and cytokine production, alterate of gene expression in host cells, and inhibit of proinflammatory responses of host cells.

近来，越来越多的证据表明抗菌肽是有效的免疫辅助因子，能够与其他的众多免疫效应子协同作用，从而起始适应性免疫，促进伤口愈合，抑制前炎症反应以及诱导和调节细胞因子和趋化因子的产生。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Aside from pancreatic enzymes activiation and autodigestive process, recent investigations have established that the upregulation of inflammatory mediator are believed to be the critical steps in the progression of mild pancreatitis to severe pancreatitis. Tumor necrosis factor-, ThyomboxaneA2 ,ProstaglandinsI2 are all important inflammatory mediator. TNF-( is one of cytokines. It is important to the over-production of ICAM-1,VCAM-1, TXA2 andPGI2. The ICAM-1 and VCAM-1 mediate both leukocytes adhesion and migration through the endothelium into tissues to connect with injuried target cells. The TXA2 and PGI2 are the production of arachidonic acid. They are responsible for the tissue ischaemia.

肿瘤坏死因子（Tumor necrosis factor-， TNF-、血栓素A2（ThyomboxaneA2，TXA2），前列环素（ProstaglandinsI2，PGI2）均是炎症反应中重要的炎性介质，其中肿瘤坏死因子-是非常重要的细胞因子之一，它由活化的单核细胞产生，在细胞间黏附分子（Intercellular adhesion molecule-1，ICAM-1）及血管细胞黏附分子(Vascular cell adhesion molecule-1，VCAM-1的表达、血栓素A2及前列环素过量生成方面起着重要的作用，前两者是介导白细胞与内皮细胞黏附，迁移于组织并与受损的靶细胞相结合的主要媒介，是炎症反应中的重要介质，后两者是花生四烯酸的代谢产物，亦是重要的炎性介质，参与构成血液循环障碍。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。