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The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变;沉淀回收的PCR产物以20μl变性上样液(95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝)溶解后热变性为两条DNA单链后,上样于8%非变性聚丙烯酰胺凝胶(含5%甘油),700伏电压、4℃电泳16小时。

Based on the current situation of rubbish production and handling in Jiangsu Province, the managing schemes for rubbish classifiable retrieval such as propaganda, legislation, management system, charge , the method of classifying, clearing have been proposed in this paper.

基于江苏省垃圾产生、处理现状,提出了垃圾分类回收的宣传、立法、管理体系、收费、分类方法、清运等管理方案。

Disposal begins with the neutralisation of airbags and other pyrotechnical components.

回收的第一步是拆除安全气囊和其它烟火部件。

In swap market, the innovated swap means are the multi-period forward established extending means, multi-period and multi-cost forward established extending means, multi-period forward option extending means, the recollectable extending means, the changeable principal extending means, the negotiated price extending means, the retail price extending means, the average price extending means, the elastic extending means, the price ceiling extending means.

在掉期市场上,商品生产者可采用多期远期启动掉期方式,多期多值远期启动掉期方式,多期远期期权掉期方式,可回收的掉期、本金可变式掉期、议价掉期、零息掉期、均价掉期、伸缩型掉期、价格上限掉期等方式。

NO RECYCLED MATERIAL IS PERMITTED, THE USAGE OF REGRIND MATERIAL MUST BE APPROVED BY XXX ENGINEER AND THE PERCENTAGE OF REGRIND IS NOT EXCEED 25%.

不允许使用回收的材料,使用重新打磨得材料必须由XXX工程师认可,打磨量不超过25

The volumes of reinfusion blood and complications were recorded.

记录回收的血液量及并发症。

Gualou recovered seeds, Trichosanthes root, Gualou Paper - to ensure product quality and price.

回收的瓜蒌籽,瓜蒌根,瓜蒌皮-确保了产品质量与价格优惠。

For the next day or two, you may feel compelled to clean out closets and recycle.

未来几天,你会有清理衣橱和资源回收的冲动。

It can recover some or all of the files it identifies.

它可以回收的部分或全部档案发现。

Of the things that can be recycled.

其中一些可以回收的东西。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。