回收物

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

So does radioactive effluent, whether from Sellafield, a nuclear reprocessing plant on the west coast of England, or the scrapyards of Russia: between 1958 and 1992, the Arctic Ocean was used by the Soviet Union, or its Russian successor, as the resting-place for 18 unwanted nuclear reactors, several still containing their nuclear fuel.

有放射性的排放物也是如此，无论是来自英格兰西岸的塞拉菲尔德核回收厂，还是来自俄罗斯的废品堆放场：在1958到1992年间，北冰洋被苏联或其接替者俄罗斯用作18个闲弃的核反应堆的搁置处，有些仍含有核燃料。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。