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The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明,该分离病毒无血凝性,在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线;人工感染28日龄雏鸡出现与临床一致的病变,并回收到病毒;应用针对IBDVVP3基因的特异性引物进行RT-PCR,能扩增到长度为1041bp的特异性目的片段;序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

For the long SRT in system, lower sludge activity had good substrate reverse and staged characters, which thus made the ERP-SBR system has good ability to resist the impact of the load.

试验发现,按照ERP-SBR方式运行可以大大减小化学除磷池的规模,而且石灰总耗量仅为城市污水后置沉淀系统的4.5%~9%,产生化学污泥含磷率为17%,相当于含73~83%磷酸盐,使化学沉积物具有较明显的回收价值。

The methods of processing waste substrate when the amount is small are as follows: replace the sawdust to be the filling material of sculpture handicraft; make the soundproofing board, ceiling, vase and so on; be used as powder for the coating, paving material or filler for plastic preparation and so on; be reused as the reinforcing material and insulated gluing material or used as the fire retardant chemical.

摘 要:我国对废弃线路板基板的回收主要是采取填埋和焚烧的方法,处理少量废弃基板时有以下几种方法:取代木屑作为雕塑工艺品的填充物;制造隔音板、天花板、花瓶等;作为粉末用于涂料、铺路材料或是塑料制备的填料等;作为增强材料和绝缘胶粘材料重新利用或做阻火剂等。

Degraded, recycled, dismantled and non-toxic and unharmful materials or of low toxic

染物的要求,优先选择易降解、易回收、易拆解、无毒、无害或者低毒、低害的

"The process includes contacting the finely ground ore with the cyanide solution, separating unwanted solids from the clear solution, and recovering the precious metals from the solution by precipitation with zinc dust."

该方法包括三个步骤,即将磨细的矿石与氰化物溶液接触;把固体物从净溶液中分离出来;通过加入锌粉沉淀从溶液中回收贵金属。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象,提取细菌基因组DNA,用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切,回收大小为1~5Kb的DNA片段,将其连接入原核表达载体pET-28a-c,最后转化宿主菌,结果成功地构建了基因表达文库,为后续的基因筛选工作奠定基础;另外,本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计,以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板,进行PCR扩增反应,结果表明成功地获得两个新基因:23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸),并进一步做了不同物种之间的分子系统发育分析,构建了基于23S rRNA基因的邻接法系统发育树,阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

The results show that in the presence of NaHCO3 as base and DMF-H2O (volume ratio 2∶1) as solvent, the complex could catalyze not only iodobenzene but also non-activated bromobenzene quantitatively at 90 ℃ without the inert gas protection, and furthermore the catalyst could be recovered and reused more than eight times.

结果表明,在没有惰性气体保护的情况下,以NaHCO3作碱,以DMF水溶液(体积比为2∶1)作溶剂,在90 ℃时,该配合物不仅对活性较高的碘代苯能够定量地催化,对未活化的溴代苯也能定量地催化,并且催化剂能够回收再利用8次以上。

Finally, the FDA acknowledges that recycling of PET or PEN by methanolysis or glycolysis results in the production of monomers or oligomers that are sufficiently pure to produce finished polymers suitable for food-contact use.

最后,美国食品药品管理局承认,回收的PET或国际笔会的methanolysis或糖酵解成果在生产单体或低聚物有足够的纯制作完成聚合物适合食品接触使用。

This NS-MCM-41-Pd proves to be an efficient heterogeneous catalyst to catalyze aryl bromides with organozinc reagents for Negishi reaction. In the third suject, fluorescent surfactants with different conjugated aromatic moieties and changeable and gradually extended hydrophobic lengths were successfully synthesized by multiple synthesized manipulations.

首先利用颗粒大小为奈米尺度(约100 nm)、表面积约 660 m2/g 与孔径大小约 2.6 nm 且孔道分布为虫洞结构之孔洞材料,NS-MCM-41,作为异相触媒担体,稼接上钯错合物形成异相催化剂 NS-MCM-41-Pd,催化 aryl bromides 与 organozinc reagents 进行 Negishi reaction,并探讨触媒反应后再回收活性测试。

Bacterial diversity of sediment in shrimp pond was analyzed by using PCR-DGGE technique, the Shannon-wiener index is 3.4971, the Richness is 41 and the Evenness is 0.94171; 17 dominant bands were excised from DGGE profile of surface sediment bacteria in shrimp pond and the V3 region of 16S rRNA gene was cloned and sequenced followed by phylogenetical analysis.

对虾池表层沉积物细菌多样性进行分析,得出该沉积环境细菌多样性指数为3.4971,丰度为41,均匀度指数为0.94171;对DGGE图谱上的特征条带进行切割回收和克隆测序后得到了优势菌的信息。

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昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

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