回合

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

In addition,the wielder of Durin's Axe may reroll one of his dice when determining who wins a fight but must accept the result of the second roll.

但是不遭受通常投回合结束-1惩罚。另外，都灵之斧使用者如果输了战斗可以再掷骰一次，但必须接受第二次的掷骰。

Sandeep: sandeep : thanks for help me to activate server home edition 2003 ur doing such a great job everyone thanx a lot!

感谢帮助我激活服务器Home Edition的2003年乌拉圭回合做这样一个伟大的工作，大家thanx了很多！

When Kai or another Scryer ally enters your party, opposing heroes and allies can't protect this turn.

当凯或者其他占星者盟军加入你的队伍时，对方英雄或盟军本回合不能护卫。

If a creature comes into play later this turn, Unstable Shapeshifter will become a copy of that creature, but it will still get +3/+3 from the Giant Growth.

若稍后在本回合中，又有一个生物进场，则不稳定变形兽会成为新进场生物的复制，且仍然得到变巨术之+3/+3加成。

Why did Ireland get so worked up by Lassana Diarra's sledging after the first leg, but not by Henry's handball?

为什么第一回合爱尔兰被皇马的迪亚拉气得够呛却忍了亨利这个？

In another exchange, the two candidates sparred over who would be the most ready to assume the position of commander in chief.

在另一回合中，两位候选人在谁最适合总统这个问题上又擦出了火花。

Beyonce knowles唱的啊 You need to stop playing round with all them clowns and the wangstas Good girls gotta get down with the gangstas Go head girl put some back and some neck up on it While I stand up in the background and check up on it Ooh Boy you looking like you what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight If you got it, flaunt it, boy I know you want it While I turn around you watch me check up on it Oohhh you watchin me shake it, I see it in ya face 'n' Ya can't take it, it's blazin, you watch me in amazement You can look at it, as long as you don't grab it If you don't go braggin, I'm a let you have it You think that I am teasin, but I ain't got no reason I'm sure that I can please ya, but first I gotta read ya Ooh Boy you looking like you like what you see Won't you come over check up on it (I'm gonna let you work up on it) Ladies let em check up on it (watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I can tell you wanna taste it, but I'm gone make you chase it Got to be patient, I like my men patient More patience, you take might get you in more places You can't be abrasive, has to know to pace it If I let you get up on it, you gotta make a promise That you gon put it on me, like no ones put it on me Don't bore me, just show me, all men talk but don't please I can be a tease, but I really wanna please you Ooh Boy you lookin like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I'm checking on you boo, do what chu do And while dance I'ma glance at this beautiful view I'm keep my hands in my pants, I need to glue em w/ glue I'm in a trance all eyes on you and your crew Me and my mans don't dance, but to feel ya'll bump and grind If won't hurt if you gone try one time They all hot, but let me see this ones mine Its slim thug and DC outta H town Ohh Boy you looking like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight x4

slimthug，你必须停止玩回合与所有这些小丑与wangstas好女孩gottagetdown 随着gangstas走头女孩把一些背部和颈部一些比它在我站起背景及检查它oohboy你看你喜欢什么，你会看到你不来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚如果你有它，炫耀它，男孩我知道你要它当我转身，你看我检查它oohhh你watchin 我摇，我看它在亚面子'n'ya不能把它，它的blazin，你看我一无所有你可以看看，只要你不抓这个问题，如果你不去braggin，我是一个让你如果你觉得我teasin，但我是不会有任何理由，我相信我能请娅但是，我首先gotta阅读娅ooh男孩找你喜欢你喜欢你见到不会你来超过清理它(我gonna让你工作起来就)让各位em检查它沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)，我可以告诉你想品尝，但我已离开人世，使你追逐它必须有耐心，我喜欢我的男病人更耐心，你也许可以得到你的地方越来越多，你不能磨料已经知道它的速度，如果我让你起床，那 yougotta做出承诺，你gonputitonme，不一样的说得对我并不负有我，只告诉我，所有男人说话，但不讨人喜欢，我可以戏耍，但是我真的想请你oohboylookin你喜欢你喜欢你见到不会你来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)我查你boo，做什么不楚而舞i'ma一眼这个美丽的看法，我把我的手在我的裤子，我需要胶水emw/胶水我在发呆都看你和你的船员和我我mans不跳舞，但感觉ya'll碰及研磨如果不会伤害如果你去尝试一次他们都热，但让我看到这样的矿井其slimthug直流outtah镇ohhboy你看喜欢你喜欢你见到不会你来清理，那(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚x4

Slim Thug You need to stop playing round with all them clowns and the wangstas Good girls gotta get down with the gangstas Go head girl put some back and some neck up on it While I stand up in the background and check up on it Ooh Boy you looking like you what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight If you got it, flaunt it, boy I know you want it While I turn around you watch me check up on it Oohhh you watchin me shake it, I see it in ya face 'n' Ya can't take it, it's blazin, you watch me in amazement You can look at it, as long as you don't grab it If you don't go braggin, I'm a let you have it You think that I am teasin, but I ain't got no reason I'm sure that I can please ya, but first I gotta read ya Ooh Boy you looking like you like what you see Won't you come over check up on it (I'm gonna let you work up on it) Ladies let em check up on it (watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I can tell you wanna taste it, but I'm gone make you chase it Got to be patient, I like my men patient More patience, you take might get you in more places You can't be abrasive, has to know to pace it If I let you get up on it, you gotta make a promise That you gon put it on me, like no ones put it on me Don't bore me, just show me, all men talk but don't please I can be a tease, but I really wanna please you Ooh Boy you lookin like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I'm checking on you boo, do what chu do And while dance I'ma glance at this beautiful view I'm keep my hands in my pants, I need to glue em w/ glue I'm in a trance all eyes on you and your crew Me and my mans don't dance, but to feel ya'll bump and grind If won't hurt if you gone try one time They all hot, but let me see this ones mine Its slim thug and DC outta H town Ohh Boy you looking like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight x4

slimthug，你必须停止玩回合与所有这些小丑与wangstas好女孩gottagetdown 随着gangstas走头女孩把一些背部和颈部一些比它在我站起背景及检查它oohboy你看你喜欢什么，你会看到你不来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚如果你有它，炫耀它，男孩我知道你要它当我转身，你看我检查它oohhh你watchin 我摇，我看它在亚面子'n'ya不能把它，它的blazin，你看我一无所有你可以看看，只要你不抓这个问题，如果你不去braggin，我是一个让你如果你觉得我teasin，但我是不会有任何理由，我相信我能请娅但是，我首先gotta阅读娅ooh男孩找你喜欢你喜欢你见到不会你来超过清理它(我gonna让你工作起来就)让各位em检查它沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)，我可以告诉你想品尝，但我已离开人世，使你追逐它必须有耐心，我喜欢我的男病人更耐心，你也许可以得到你的地方越来越多，你不能磨料已经知道它的速度，如果我让你起床，那 yougotta做出承诺，你gonputitonme，不一样的说得对我并不负有我，只告诉我，所有男人说话，但不讨人喜欢，我可以戏耍，但是我真的想请你oohboylookin你喜欢你喜欢你见到不会你来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)我查你boo，做什么不楚而舞i'ma一眼这个美丽的看法，我把我的手在我的裤子，我需要胶水emw/胶水我在发呆都看你和你的船员和我我mans不跳舞，但感觉ya'll碰及研磨如果不会伤害如果你去尝试一次他们都热，但让我看到这样的矿井其slimthug直流outtah镇ohhboy你看喜欢你喜欢你见到不会你来清理，那(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚x4

If the Doha round collapses, regionalism, despite its unarguable economic inferiority, will replace multilateralism as the organising principle of global trade.

如果多哈回合谈判破裂，那么区域主义将取代多边主义成为全球贸易谈判的组织原则，尽管区域主义在经济上的劣势毫无争议。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷