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130 rats were randomly divided into five groups: 5F electroacupuncture treatment group, 50Hz electroacupuncture treatment group,100Hz electroacupuncture treatment group, the model group and the control group. 4mm defect was produced in the left sciatic nerve of each rat and silicon nerve guards were used to bridge the nerve defect not including the rats of the control group. After 10 weeks electroacupuncture treatment, gross observation, histological examination of the sciatic nerve were carried with method of HE stainning and S-100 immunhistochemical staining, Sevier-Munger stainnning. The histomorphometric examination of the triceps muscles were observed with measuring width muscle cell and the wet weight, and measuring the content of the heparin and the lactic aid of the triceps muscle with biochemistry methods.

实验方法:将130只Wistar大鼠随机分为五组:正常组、造模组、电针5Hz组、电针50Hz组、电针100Hz组,除正常组外,其余各组手术切取坐骨神经4mm后,做坐骨神经硅胶管桥接术,造成周围神经损伤模型,取患侧足三里、三阴交、环跳穴,术后第四天开始电针,每两天一次,每次10分钟,治疗10周后处死动物,取出硅胶管内新生的坐骨神经,纵切片,HE染色、S100免疫组化染色、Sevier-Munger银染,观察坐骨神经纤维再生情况,以健侧同段坐骨神经做对照;取患侧脖肠肌称取湿重,HE染色,观察腓肠肌肌细胞直径,生化测量腓肠肌肌糖元、乳酸。

From Chaotianmen to Zigui,the Shannon-Weaver diversity index of phytoplankton changes undeely.4 the impact of sediment deposition on zooplankton in the waters studiedAccording to the results of the experiment, we had learnt that the species composition of zooplankton sampled had the same tendence-the species was from less to more, and the biomass was also gradually increasing. Particularly Copepoda had increased significantly in the type and quantity. The main of zooplankton at each sampling situs were protozoa and rotifers.

朝天门江段到秭归江段的浮游植物的Shannon-Weaver多样性指数呈波浪式变化,整体有下降趋势。4研究水域泥沙沉降对浮游动物的影响从本次实验的结果来看,四次采样浮游动物种类组成都有同一个趋势:种类由少到多,生物量也逐渐增大,特别是桡足类在种类和数量上都增加明显,各采样点的浮游动物以原生动物和轮虫为主。

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