四裂体的

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

It is mainly used to control the second-generation schizont of coccidis with the activity peak to coccidis on the forth day.

尼卡巴嗪主要作用于球虫第二代裂殖体，对鸡虫的活性高峰期为第四天，即对第二代裂殖体活性最强。

According to the relevant datums and field investigations, the paper makes No. 316 national highway in South region as research region to analyze the conditions of inoculating disasters and slope s transform destruction model and draws such conclusions:(1)Many factors such as terrain, geomorphic, lithosome and solum character, geologic structure, neotectonic movement, rainfall condition, groundwater trait, anthropokinetic and so on influence on the study region, which make the geological disasters occurred frequently and generate enormous hazards.(2)The geological disasters have such distribution characteristics: terrain and tectonic make geological disasters development of osculating correlated property, relative concentrate property, packet property, periodic property and so on.

本文首先在搜集相关资料和野外调查的基础上，以陕南316国道公路为重点研究区，全面分析了研究区域内地质灾害的孕灾条件与斜坡变形破坏模式，得到了以下认识：(1)研究路段地质灾害的发生发展主要受控于区内地形地貌、岩土体特性、地质构造与新构造运动、降雨条件、地下水特征及人类工程活动等因素：(2)研究路段发育滑坡、崩塌、泥石流和危险斜坡等四种地质灾害，具有如下的分布特征：地势与构造对地质灾害发育的密切相关性、相对集中性、群发性、周期性等；(3)研究路段斜坡地质灾害主要有六种变形破坏模式分别为：顺层滑动破坏、蠕滑—拉裂变形破坏、弯曲—拉裂—剪断破坏、旋转式滑移—拉裂破坏、楔形体滑动破坏、堆积层沿基岩面的滑动破坏模式，而且斜坡灾害的变形破坏往往是多种模式的组合。

A high performance liquid chromatographic method was established for determination of caffeic acid contents in tetraploidy taraxacum officinale and diplontic taraxacum dissectum.

本文采用四倍体药蒲公英和二倍体多裂蒲公英为材料，通过HPLC法测定并比较两种蒲公英中咖啡酸的含量，以大力推广产量高，抗逆性强，药用活性成分高的优质多倍体蒲公英新品种。

The optimization of heat shock conditions for producing tetraploidy loach was treated the fertilized eggs for 4 min with 41℃ water after fertilization about 20 minutes.

采用热休克抑制受精卵第一次卵裂、Ag-NOR计数鉴定四倍体胚胎的方法，探索了诱发四倍体泥鳅的最佳条件是：在受精后20 min左右采用41℃热水持续处理泥鳅受精卵4 min。

It indicated that the yield of tetraploidy not more than 25% induced by inhibiting the first mitosis with CB, colchicine and 6-DMAP, respectively, while it can be induced with higher percentage by blocking PB1releasing with CB. The tetraploidy percentage of groups shock began at 15—22min after fertilization are higher than that shock began at 42min, and the yield of tetraploidy also increased with the CB density and time lasting.

结果表明，CB、6-DMAP和秋水仙素抑制扇贝第一次卵裂诱发四倍体的比例低于25%；采用CB抑制PB1可有效地诱导产生四倍体，从授精后42min提前到15—22min开始处理，抑制PB1的放出有助于提高四倍体的比例，在12℃，处理开始和终止时间分别在授精后20—22min和62—67min时(即PB2始出现时)，面盘幼虫四倍体率最高，为56.5%。

Fruit smooth, strongly 4-angled, with 4 bands of white trichomes separated by glabrous replum and valve midveins

果平滑，强烈四棱形，白色毛状体条纹状的具4 以无毛假隔膜和裂爿中脉分开 9 E。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。