四组

In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification， iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry， 2D-LC-MS/MS)对雌二醇(17β-oestradiol， E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较，共鉴定到置信度在95%以上的蛋白质576种，其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质，其中10种上调，16种下调。

Part 2:(1). Twenty-four rats were divided into 4 groups and wereadministrated uric acid, oxonic acid, oxonic acid + uric acid (OA+UA group) and oxonic acid + pyrazinamide (OA+PZA group) i.p.

天津医科大学博士研究生学位论文第四部分：将30只大鼠随机分为五组，在腹腔注射氧嚓酸+毗嚓酞胺建立HU模型的基础上，分别用中汇痛风定、中药l、中药2和中药3每日灌胃1次。7天后比较尿酸代谢的变化。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

We conducted a nested case-control study within four large, prospectie cohorts to inestigate whether prediagnostic plasma leels of IGF-I, IGF-II, and IGFBP-3 were associated with pancreatic cancer risk. Plasma leels in 212 cases and 635 matched controls were compared by conditional logistic regression, with adjustment for other known pancreatic cancer risk factors.

我们在四个大型的前瞻性群组中进行了巢式病例对照研究以调查是否诊断前期的血浆IGF-I， IGF-II，和 IGFBP-3水平与胰腺癌风险相关。212病例和635配对对照的血浆水平使用条件逻辑回归进行比较，使用另外已知的胰腺风险因子进行调节。

Differences among larval durations were significant, while no significant difference in pupal duration were detected.

喂 10 %蜂蜜时，雌蛾寿命、交配率和产卵量在四个幼虫食料组之间没有显著差异。

The geometric and electronic structures of quadruply bonded metal dinuclear compounds M2Cl4(PMe3)4 (M=Cr, Mo, W) and Mo2X4(PMe3)4 (X=F, Cl, Br, I) have been investigated by means of density functional theory method with TZ2P-STO basis sets. It was shown that the title compounds can be accurately predicted by DFT included ZORA relativistic correction.

采用密度泛函理论方法，在TZ2P-STO基组水平下，对金属四重键化合物M2Cl4(PMe3)4 (M=Cr， Mo， W)和Mo2X4(PMe3)4 (X=F， Cl， Br， I)的几何结构进行优化，分析了电子结构，并运用TDDFT方法对其低占据激发态进行了计算。

Article 41-~Upon reverification by special organs or personnel of audit institutions, the audit reports submitted by audit teams shall be finalized through examination by audit institutions and handled in accordance with the following provisions

第四十一条审计组提出的审计报告，经审计机关专门机构或者人员复核后，由审计机关审定并按照以下规定办理

Pushkin writes down (1821),"prisoner"(1822)"cause ocean ""dirk "(1824th) rank of sheet, long poet having written a group of "south poem ", including "Caucasian to capture "(1822),"robber brother "(1822),"tear spring that Bach Qiesala leans on"(1824),"puncture vine ridge "(1824) four sheets romantism narrating.

他追求自由的思想更明确，更强烈了。普希金写下《短剑》（1821）、《囚徒》（1822）《致大海》（1824）等名篇，还写了一组&南方诗篇&，包括《高加索的俘虏》（1822）、《强盗兄弟》（1822）、《巴赫切萨拉依的泪泉》（1824）、《茨冈》（1824）四篇浪漫主义叙事长诗。

Pushkin writes down (1821),"prisoner"(1822)"cause ocean ""dirk "(1824th) rank of sheet, long poet having written a group of "south poem ", including "Caucasian to capture "(1822),"robber brother "(1822),"tear spring that Bach Qiesala leans on"(1824),"puncture vine ridge "(1824) four sheets romantism narrating.

普希金写下《短剑》（1821）、《囚徒》（1822）《致大海》（1824）等名篇，还写了一组&南方诗篇&，包括《高加索的俘虏》（1822）、《强盗兄弟》（1822）、《巴赫切萨拉依的泪泉》（1824）、《茨冈》（1824）四篇浪漫主义叙事长诗。

Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中，发现rottlerin抑制鸟胺酸去羧化活性最具成效，之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性，因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后，细胞进行计划性死亡，并可侦测到DNA片段、细胞核浓缩及sub-G1提增；而细胞处理药物十小时后与控制组细胞比较，观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上；除此以外，测定鸟胺酸去羧化去磷酸化的情况，发现於酥胺酸位置有显著差异，而丝胺酸和酪胺酸位置并无差异。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。