四组

Methods Totally 20 health female rats were randomly and equally divided into control group, fimbria/fornix transection and ovariectomized group, ovariectomized group and fimbria/fornix transaction group.

取健康雌性Wistar大鼠20只，随机分成四组，对照组，穹隆海马伞切断加卵巢切除组，卵巢切除组，穹隆海马伞切断组每组各5只。

Methods: First of all,Wistar rat model of iodine deficiency was made.Then rats were randomly divided into four groups:normal iodide group, high iodide group, normal iodate groupand high iodate group.

本实验在成功复制碘缺乏Wistar大鼠动物模型的基础上，将大鼠随机分成四组：适量碘化钾组、过量碘化钾组、适量碘酸钾组和过量碘酸钾组。

Methods The cognitive impairment rat model was induced by hypodermic D-galactose injection and half fat feeder (6 weeks), the Morris water maze was used to screen the model rats, which were divided randomly into 4 groups (12 rats in each group): the model group, the hydrochloricdonepezil group (0.4 mg/kg, weight, sic passim), the low-dose Huannao Yicong Fang group (7 g crude drug/kg), the high-dose Huannao Yicong Fang group (14 g crude drug/kg), they were treated respectively by intragastric administration. Besides, a control group of 12 rats was set up, they were given tales doses of distilled water. After 4 weeks, the behavior of the rats was detected, the contents of plasma P-selectin and PAI-1 were measured by ELISA, also the hemorrheology was measured.

采用皮下注射D-半乳糖及喂饲半高脂饲料（6周）的方法造成大鼠认知功能障碍，Morris水迷宫行为学测试确定模型成功的动物，随机分成四组（每组12只：模型对照组给予等量水，盐酸多奈哌齐组（0.4mg/kg，体质量，下同），还脑益聪方低剂量组（7g生药／kg），还脑益聪方高剂量组（14g生药／kg），同时灌胃给药，并设立正常对照组，给予等量水。4周后，对大鼠行为学检测，应用酶联免疫法检测大鼠血浆P-选择素及PAI-1含量和血液流变学各项指标。

Methods The patients were divided into 4 groups by thefracture type: simple compression fracture group, burst fracture group,dislocation group and multiple segment spinal injury group.

方法将手术组按不同的骨折类型分为四组：压缩骨折组，爆裂骨折组，骨折脱位组，多节段脊柱损伤组。

The firest group was called "the leader of technology", and the second group was called "the potential leader of technology", and the third group was called "the protagonist of technology",and the fourth group was called "the straggler of technology".

这些国家按照技术发展的水平从高到低共分为4组，第一组为"技术领先者"；第二组为"技术潜在的领先者"；第三组为"技术积极采用者"；第四组为"技术落后者"。

Results showed that the intake of inulin decreased the activity of aldose reductase, glyoxalase I and sorbitol dehydrogenase in the plasma and liver.

实验共进行四周，分为四组(1)Control组(2)Diabetes组(3) Diabetes+inulin组(4) Control+inulin 组，实验期间给予每只小鼠每日5克饮食摄取量并添加浓度11%的菊醣及自由饮水。

According to the results of B-mode ultrasonography, 110 cases were disparted for four groups: carotid arteries normal, carotid arteries incrassation, stabilization plaque and unstabilization plaque.

据颈动脉超声结果将患者分为四组即颈动脉正常组、颈动脉内膜增厚组、稳定斑块组、不稳定斑块组。

Methods Eighty healthy nulliparous parturients scheduled for patientcontrolled epidural analgesia in labour, were randomly divided into 4 groups(n=20) according to different infusion speed:group I with a speed of 4 ml/h, group II with 6 ml/h, group III with 8 ml/h, group IV with 10 ml/h.

对80 例行自然分娩的单胎足月初产妇，均采用硬膜外自控分娩镇痛，根据不同背景输注速率随机分为四组，A组输注速率为4 ml/h，B组为6 ml/h，C组为8 ml/h，D组为10 ml/h。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养，22小时后上述各组卵母细胞的核成熟率分别为75.2％、73.1％、69.8％、63.5％，处于第一次减数分裂末期的比率分别为16.3％、15.9％、16.9％、27.0％，在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P＜0.05)，处于末期的比例明显低于其它各组(P＜0.05)：添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6％、66.6％、73.6％)明显高于BSA组的正常率43.3％(P＜0.05)；各组CG发生迁移较好的卵母细胞的比率分别为58.8％、33.9％、54.7％、47.9％，血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P＜0.05)。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。