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Results (1) Compared with that of 4-Retro-decoction group and heart failure control group, the activity of Na〓-K〓-ATPase in low- and middle- dosage ginger group showed to be decreased with statistically significant difference. P<0.05.(2) The activity of Ca〓-ATPase and Mg〓-ATPase in the lower- and middle-dosage ginger group were not statistically different from that of 4-Retro-decoction group and control group, but the activity of Ca〓-ATPase showed an ascending tendency in low-dosage ginger group.

结果 (1)低、中剂量干姜组的Na〓-K〓ATP酶活性明显偏低,与四逆汤组和心衰组比较,差别均具有显著性,P<0.05;(2)低、中剂量干姜组的Ca〓-ATP酶和Mg〓-ATP酶活性与四逆汤组和心衰对照组比较,无统计学意义,但低剂量干姜组的Ca〓-ATP酶与其它三组相比有升高趋势。

Objective To investigate the difference between the effects of Ginger Extract and4-Retro-deˉcoctionon cardiac systolic/diastolic function of rabbit with heart failure.Methods 40pure-breed New-Zealand rabbits were randomly divided into4groups:low(0.028mL/kg),middle(0.056mL/kg),high(0.112mL/kg)dosage ginger group and4-Retro-decoction group.The heart failure model of rabbit was established by intravenous administration of20g/L pentobarbital sodium at a certain rate.Oral tube perfusion of ginger extract and4-Retro-decoction were administrated after the establishment of the model.Their hemodynamic changes were detected before and after the model establishment,and at5,10,15,20,30,45,60,90,120,150minutes respectively after the ginger administration by a RM-6004-graph physiological monitor.

目的 探讨干姜提取物和四逆汤对心衰兔心脏舒缩性能的作用异同方法以40只纯新西兰兔研究对象,随机分为高、中、低剂量干姜组和四逆汤组共4组,用恒速滴注戊巴比妥钠的方法建立兔急性心力衰竭模型,造模成功后,经口插管入胃灌服受试品,用RM-600四道生理仪测定造模、造模成功时及用药后5min、10min、15min、20min、30min、45min、60min、90min、120min、150min兔的心肌舒缩性能指标,观察各时段指标的变化情况。

in order to probe into whether glycine supplementation plays an adjusting role in sports performance and immune functions, the authors divided 30 mice randomly into an exhaustive exercising group (e1), glycine supplemented exhaustive exercising group (e2) and a normal resting control group, with 10 mice in each group, supplemented glycine to mice in group e2 and physiological saline to mice in the other two groups, let mice in groups e1 and e2 do an exhaustive swimming exercise 4 weeks later, applied the trace ch50 hemolysis method to analyze supplement activity, applied the mtt method to perform a quantitative analysis on the lymphopoiesis ability of spleen, and thus revealed the following findings: compared with mice in group e1, the time taken by mice in group e2 to swim to an exhausted state is significantly longer (p.05); total supplement activity in blood serum of mice in group e1 is significantly lower than the same of mice in group s (p<0.01), and total supplement activity in blood serum of mice in group e2 is significantly higher than the same of mice in group e1 (p.01), but still significantly lower than the same of mice in group s (p.05); as observed from the test of the lymphopoiesis ability of spleen, the lymphopoiesis ability of spleen of mice in group e1 is significantly lower than the same of mice in group s, and the lymphopoiesis ability of spleen of mice in group e2 is significantly higher than the same of mice in group e1, but still significantly lower than the same of mice in group s.

四型肽精氨酸脱氨酶在类风湿性关节炎。。。以马来酸酐与氨水为原料热缩聚法合成。。。摘要:为探讨甘氨酸补充对运动能力及免疫功能是否具有调节作用,将30只小鼠随机分成力竭游泳运动组(e1)、甘氨酸补充后力竭游泳组(e2)和静息正常对照组,每组10只。在e2组补充甘氨酸,其余两组给予生理盐水,处理4周后,e1组和e2组均做力竭性游泳运动。采用微量ch50溶血法分析补体活性,采用mtt法定量分析脾脏淋巴细胞增殖能力。结果显示,与e1组比较,e2组游泳至力竭的时间显著延长(p<0.01)。e1组血清总补体活性显著低于s组(p<0.01),e2组显著高于e1组(p.01),但仍显著低于s组(p.05)。在脾脏淋巴细胞增殖试验中观察到,e1组显著低于s组,e2组显著高于e1组但仍显著低于s组。

It will provide theoretical and experimental base for the research of syndrome of traditional Chinese medicine. Method: 60 depuratory and healthy SD female rats with normal estrous cycle(4~5days),3-month in age, 230±20g in body weight,were divided into 6 groups randomly, normal group,model group,low-dose group,middle-dose group, high dose group,control group.

选用动情周期正常(4~5天)的健康清洁级SD雌性大鼠60只,鼠龄3个月,体重230±20g,随机分为6组,每组10只:即正常对照组,模型对照组,模型加加减温经汤低剂量组,模型加加减温经汤中剂量组,模型加加减温经汤高剂量组(简称为&高剂量组&,低剂量组、中剂量组、高剂量组又合称为&治疗组&),桃红四物汤组。

The results showed that: at metaphase I, the homoeologous chromosome pairing among different F1 hybrids ranged from 2.0 to 11.4 bi-valents formed by homoeologous chromosomes per pollen mother cell, and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all biva-lents were disjoined and most univalents were divided.

结果表明在中期I阶段,这些杂种一代的近缘染色体联会变化很大,每个花粉母细胞中二价体形成的数目从平均2个到11.4个不等,甚至在某些花粉母细胞中,还发现极少的多价体和非部分同源染色体所形成的单基因组内二价体;在后期I时,所有的二价体分离,同时多数单价体也分离,分离的二价体和分离的单价体都移向两极,从而形成两组染色体;因为这时完整花粉母细胞中分离的二价体在两组染色体中总是对应出现,从而根据半二价体上染色体重组的位置可以分析在二价体的四分体时期发生在非姊妹染色体之间的多种染色体交换类型,如单交换、三线双交换、四线双交换、四线三交换和四线多交换。

Each variety was sown into three blocks.They were harvested at 79~83d(MS1), 86~89d(MS2),93~96d(MS3) and 98~100d(MS4) after seeding and ensiled with four different ensiling treatments(R group:adding lactobacillus,3×10~9cfu/t,E group: adding cellulase 2×10~4u/kgDM,xylanase 6×10~5 thousand u/kgDM andβ-glucanase 5×10~5 u/kgDM,R+E group:adding lactobacillus 3×10~9cfu/t and enzyme preparation as E group together,group:adding no additives but carrier).The whole crop rice were pestled by mallet and then chopped into 3cm~5cm after they were harvested and then ensiled in a 6L polyethylene pot and sealed.

为探索全株青贮饲料稻的适宜品种、适宜收获时期和适宜青贮处理方法及其品质评价,2006年,选择饲料稻湘早籼24、湘丰早119和浙辐802,采用相同的栽培技术,种植在同一大田,每个品种设三个重复小区,分别在四个不同成熟阶段(MS1:播种后79~83d;MS2:播种后86~89d;MS3:播种后93~96d;MS4:播种后98~100d)收割,采用四种青贮添加剂处理(R组:添加乳酸菌3g/t;E组:添加纤维素酶2万u/kgDM、木聚糖酶60万u/kgDM和β-葡聚糖酶50万u/kgDM;R+E组—添加乳酸菌+复合酶,其中乳酸菌添加量同R组,复合酶添加量同E组;K组—不添加任何添加剂)。

This may be explained by follows: First, Decoction Shenkangling can regulate the homeostasis of NEI network and reduce the increased activity of NF-κB to restrain the action of inflammatory-sclerotic media; Second, it can redress the disequilibrium of the ratio of TXA2/PGI2 to reform the hypercoagulable state; Third, it can enhance albumin and reduce cholesterol and triglyceride; Fourth, it can amend the renal damage and protect the function of renal tubule and stroma to prevent the transformation from MCD to FSGS; Last, it can lessen urine protein in AN rats markedly and relieve the side effects of prednisone.4. NF-κB, TXA2 and PGI2, along with the renal pathomorphology and so on can all be the indexes of the diagnosis of Symptom of "Kidney Deficiency and Blood Stasis" and the application of Methods of "Benefiting the kidney and Promoting blood circulation" in TCM.5. The curative effects on AN rats by treatment of integrating Traditional Chinese Medicine with prednisone are much better than using prednisone only. And the curative effects on AN rats by treatment of matching Decoction Shenkangling, having the function of "Benefiting the kidney and Promoting blood circulation", with Prednisone are much better than using Prednisone only.6. When it referred to decreasing urine protein, cholesterol and triglyceride and increasing albumin, Decoction Zhibodihuang excesses Decoction Taohongsiwu. However, when it referred to regulating the disequilibrium of TXA2/PGI2 and meliorating renal lesion, Decoction Taohongsiwu exceeds Decoction Zhibodihuang.7. The curative effects on AN rats by treatment of matching Methods of "Benefiting the kidney and Promoting blood circulation" with Prednisone overmatches using Prednisone matching Method of "Benefiting the kidney" or Method of " Promoting blood circulation" only.

益肾活血中药肾康灵能显著改善AN鼠的肾虚血瘀证的临床表现和实验指标,这可能与中药肾康灵能调理AN鼠机体的NEI网络功能稳态,降低异常升高的NF-κB活性,抑制免疫炎症/硬化介质环节,纠正失衡的TXA2/PGI2,改善AN鼠血液高凝状态,提高AN鼠的血清Alb,降低Ch和TG,减轻AN鼠的肾脏组织病理学变化,保护肾小管-间质功能,阻止MCD向FSGS转化;显著减轻AN鼠的尿蛋白水平以及减轻激素副作用有关。4、NF-κB、 TXB2和6-keto-PGF1α以及肾脏病理形态学改变等,都可作为中医肾虚血瘀证诊断和益肾活血法应用的客观指标。5、中西医结合干预AN鼠疗效优于单纯激素治疗;强的松配合益肾活血中药肾康灵组的综合疗效显著优于单纯强的松组。6、知柏地黄汤组在降低尿蛋白、提高血清Alb、降低Ch和TG疗效上优于桃红四物汤组;桃红四物汤组在纠正失衡的TXA2/PGI2和减轻肾脏病理损害方面优于知柏地黄汤组。7、激素配合益肾活血法干预AN鼠在一定程度上优于激素配合益肾法或活血法。

The relations between MAP2 and epileptogenesis were also explored.Methods Model of epileptic rat was established by Pentylenetetrazol and divided into PTZNA group,PTZDiazepam group, DiazepamPTZ group and normal control group.

建立戊四氮致疒间大鼠模型,分为戊四氮生理盐水组、戊四氮地西泮组、地西泮戊四氮组及正常对照组。

The relations between MAP2 and epileptogenesis were also explored. Methods Model of epileptic rat was established by Pentylenetetrazol and divided into PTZ-NA group, PTZ-Diazepam group, Diazepam-PTZ group and normal control group.

建立戊四氮致癎大鼠模型,分为戊四氮生理盐水组、戊四氮地西泮组、地西泮戊四氮组及正常对照组。

Methods Twenty SD rats were randomly divided into two groups. The left phrenic nerve or accessory nerve was transferred to femoral nerve by the vascularized ulnar nerve bridging in Group A and Group B, whereas the right femoral nerve remained the disjunction. The electromyogram and wet weight, throughput and section area of myelinated nerve fibers, and section area of muscle fibers in quadriceps femoris muscle were determined after 24 weeks.

大鼠20只,随机分为A、B两组,每组10只。A组:左侧行膈神经桥接带血管蒂的尺神经转位修复股神经手术,右侧切断神经不吻合,作空白处理。B组:左侧行副神经桥接带血管蒂的尺神经转位修复股神经手术,右侧切断神经不吻合。A、B两组于24周后观察股四头肌电生理、有髓神经纤维通过率及截面积、股四头肌湿重比、肌纤维截面积比等指标。

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