四碱的

Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments. Ligation of these four fragments resulted in a cloned DNA MgE717 coding for the 29～268 amino acid region of gE. Sequencing result showed that there is a mutation at 255 amino acid, Asn instead of Asp.

分四段分别合成，基因拼接得到了改造后的基因片段MgE717，经测序，除了在gE基因+763位核苷酸处存在一碱基置换，导致第255位的氨基酸残基由Asp突变为Asn，其余的序列与PrV鄂A株gE基因的氨基酸序列相符。

The pigment in colored cotton fibers was extracted by methanol at room temperature. The results of color reactions HCl-Mg reaction, NaBH4 reaction, NaOH reaction, FeCl3 reaction and Pb(Ac2 reaction of the pigment extractive showed that the pigment extracted by methanol was flavone or flavonol with two adjacent hydroxide groups (Table 2, Fig. 1). The results of UV spectral analysis further proved that the pigment extracted by methanol from brown fiber belonged to flavone with two adjacent hydroxide groups in B ring, and that from the green fiber contained not only flavone but also flavonols with two adjacent hydroxide groups both in A ring and B ring and indican replaced a hydroxide group at 3-site of A ring Fig.

通过盐酸－镁粉反应、四氢硼钠还原反应、碱反应、醋酸铅反应、氯化铁反应和紫外光谱吸收的分析表明，甲醇室温提取的棕色棉纤维色素可能是黄酮类化合物，B环上带有邻二酚羟基；甲醇室温提取的绿色棉纤维色素除黄酮类化合物外，还含有3-位羟基被糖甙化的黄酮醇类化合物，A、B环上都带有邻二酚羟基。

To avoid the permutable model of protein, the 43 Table of Triplet Codes Given by F.H.C.Crick in 1969 is in fact that one group of permutations versus the permutation elements in another group of permutations , which is a typical wrong application and a wrong calculation.

Crick 43 遗传密码表回避蛋白质分子的排列组合模型问题，根据氨基酸种类数20 和 DNA 上碱基种类数 4进行对四元素选三元素的排列组合计算建立" DNA ～蛋白质"间的一一对应，本质上是排列组合应用中一组排列组合对应另一组排列组合的具体元素。

On the normotensive rat, pretreatment with verapamil (0.25 ug/0.1 ul/site), the blocker of calcium channels, by microinjection into the rVLM bilaterally, not only caused a complete block effect on the elevation of BP, HR and the pressor response to defense stimulation by the subsequent microinjection of physostigmine, glucocorticoid or mineralocorticoid (the same dosage as mentioned above) into the same sites, but also led to a slight reduction on the basal cardiovascular activities to a certain extent.

四、在正常血压大鼠上预先用钙通道阻断剂维拉帕米(0.25μg/0.1μl/site)微量注射至双侧rVLM，不仅能阻断随后在同部位微量注射毒扁豆碱(0.4μg/0.1μl/site)、肾上腺糖、盐皮质激素(分别为40ng/0.1μl/site)所致的升压、加快心率、以及电刺激dPAG诱发的防御性升压反应，而且能引起上述指标不同程度的较弱的降低。

Starting from 3, 5-dihydroxybenzoic acid, a total of 16 kinds of salicylicaldehyde derivatives have been synthesized by the esterification, substitution, reduction, formylation, condensation, brominate, functional group protection and deprotection reactions, of which 11 have not been reported yet. Enatiomerically pure 2-amino-2'-hydroxy-1, 1'-binaphthyl has been easily obtained from its racemic mixture by the eantioselective formation of inclusion crystals with N-benzylcinchoninium chloride.

树枝状大分子希夫碱三齿手性配体的设计与合成从3，5-二羟基苯甲酸出发，通过酯化、取代、还原、甲酰化、缩合、官能团的保护与去保护等反应合成了四个系列16种水杨醛衍生物，其中11中是未见文献报道的新化合物。

HepG2 cells were exposured to oxymatrine, final concentrations of which were 0.1, 0.2, 0.3, 0.4, 0.5, 0.8, 1, 2, 5, 8, 10, 15 g.L-1 respectively, MTT method was used to determine the inhibiting effects of oxymatrine on HepG2 cells proliferation, followed by Haematoxylin and Eosin staining to observe the cell morphology.

以0.1，0.2，0.3，0.4，0.5，0.8，1，2，5，8，10，15 g.L-1的氧化苦参碱处理HepG2细胞，四噻唑蓝法检测其对细胞增殖的影响；苏木精-伊红染色比较细胞形态差异；免疫组化染色进一步检测HepG2细胞肿瘤相关蛋白表达量的变化。

In this paper, the bio-ecological characteristics of indigenous halophytes: Salsola implicata, Suaeda linifolia, Halocnemum strobilaceum and Kalidium foliatum will be studied in order to explore the mechanisms of plants tolerance and to get the theory and measures for treating salt soil and applying halophytes.

本文拟通过对生长于盐渍化土地上的四种常见盐生植物：盐爪爪、盐节木、亚麻叶碱蓬和密枝猪毛菜的生物生态学特性的研究，查明盐生植物适应盐渍化土壤的机制，为在盐渍化土地上恢复、重建和扩展植被，并为改造该类土地提供依据。

Reflecting layer is composed of TiO2 large nano-particles with mean grain size of 250nm or 150nm prepared using organic alkali tetramethylammonium hydroxide as peptizer. Nano-crystalline TiO2 porous thin film composed of small nanocrystalline (10~20nm) was used as a substrate layer to absorb large amount of dye molecules.

用有机碱四甲基氢氧化铵做胶化剂制备的两种大粒径纳晶TiO2（粒径为250nm和150nm），用它们分别制备薄膜做为反射层；用小粒径纳晶TiO2(10~20nm)来制备的纳晶多孔薄膜为底层膜，用于吸收大量的染料。

On the base of structure and constitute analysis of organic chromium of nicotinate chromium and 〓, which all have evidently biological function, it reports the preparation of the first type of four kinds new organic chromium complexes . H〓O,. H〓O,,, in which Salen schiff base ligand and nicotinate or isonicotinate, or pyromucate, or benzoate ligand are contained simultaneously.

根据具有明显生物学功能的有机铬——烟酸铬和〓配合物的组成及结构特征，合成了第一类四种含有希夫碱Salen配体和烟酸、异烟酸、呋喃甲酸和苯甲酸的有机铬，其组成分别为。H〓O，。H〓O，，。

Cytoplasmic actin microfilament systems were localized in the freshly isolated onion protoplasts of bulb parenchyma cells using TRITC labelled phalloidin probe in the present study.

使用四甲基罗丹明标记的鬼笔环碱探针，以新分离的洋葱鳞茎薄壁细胞原生质体为材料，观察了细胞胞质肌动蛋白微丝骨架的结构与形态。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。