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四碘荧光素

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We first studied the interaction mechanism of Erythrosin B with proteins by spectrophotometry and fluorospectrophotometry, calculated their binding number, binding constants and binding site, and estimated their binding type.

将分光光度法和荧光光度法结合,首次研究了四碘荧光素与蛋白质的作用机理,计算了结合数、结合常数和结合位置,判断出结合类型。

Yunnanensis. The pollens of dehisced anthors of flowering P. polyphylla plants was slightly painted on the slide glass, the activities of P. polyphylla pollens were determined resp. by iodine-potassium iodide dyeing method (I2-KI), triphenyl tetrazolium chloride staining method, aceto carmine dyeing method and fluorescein diacetate method with a little improvement.

方法]将滇重楼开花植株裂开花药的花粉轻轻涂抹载玻片上,分别用碘-碘化钾(I2-KI)染色法、氯化三苯四氮唑染色法、醋酸洋红染色法和略有改动的荧光素二醋酸酯法对滇重楼花粉活力进行侧定。

METHODS: After anaesthetized, rabbits were cut at the skin for 2 cm long at the crossing of skull center and posterior canthus, skull was exposed and periosteum was separated, then a round skull window with diameter of 0.5 cm was drilled at 0.5 cm left or to sagittal suture and 0.5 cm posterior to coronal suture, after that, 35 g/L rose Bengal was slowly injected from ear-edge vein in dosage of 1 mL/kg by once.

兔麻醉后,沿颅骨的中央与眼后角垂直交叉处纵行切开皮肤约2 cm,暴露颅骨,刮离骨膜,在矢状缝左侧外0.5 cm与冠状缝后0.5 cm处,用钻头直径0.5 cm的颅钻钻透颅骨,造成颅骨圆形洞窗,随即由耳缘静脉按1 mL/kg一次性缓慢注入35g/L四氯四碘荧光素钠盐。约3 min后用冷光源(波长540 nm,功率140 lx)对准颅骨洞窗,连续照射8 min后,缝合皮肤切口。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。