四囊的

But the abnormal phenomena in autotetraploid rice were more than that in diploid rice.

同源四倍体水稻胚囊发育过程与二倍体基本一致，但在同源四倍体水稻胚囊的形成和发育过程中出现了较多的异常现象。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Due to effect of temperature, the differentiation of clinandrium began when bud were about 1.6-2 mm, after then all buds differentiated clinandriums, and one anther could differentiate to 1 ~ 4 clinandriums in which there were microspores which in stage from tetrad to mature pollen grains.

由于温度的影响，1.6～2mm大小的花蕾开始出现花粉囊的分化，以后各大小的蕾都分化了个数不等的花粉囊，一个花药可分化出1～4个花粉囊，可见到自四分体到成熟花粉粒的各阶段小孢子。

Toreniafournieri. has a naked embryo sac that protrudes from the micropyle into the cavity between the funiculus and placenta. As embryo sac is mature, the egg cell, the two synergids and about half the central cell are located outside the micropyle.

蓝猪耳在其发育的四核期，胚囊从珠孔端伸出，当胚囊完全成熟时，助细胞、卵细胞和大部分中央细胞均暴露于胚珠组织之外，是研究被子植物受精和胚囊发育的模式植物。

Past study re-sults had indicated that MMC in the apomictic type could at most develop to te-trad stage.We observed that the sexual embryo sac of"Pingyi Tiancha"coulddevelop into 4-nuclei embry sac.

前人只观察到无融合生殖类型的大孢子母细胞至多能发育到四分体时期，而我们观察到平邑甜茶的有性胚囊能发育到四核胚囊。

Accumulation experiments were performed with:(1) Scenedesmus quadricanda as single food;(2)a 50:50 mixture of Scenedesmus quadricanda and Microcystis aeruginosa based on carbon concentration; and (3) Microcystis aeruginosa as single food. MCs inside Bellamya aeruginosa were tested by ELISA, and a curve for the dynamic variation of MCs is obtained.

毒素积累试验分别以单一四尾栅藻、50%铜绿微囊藻+50%四尾栅藻的混合藻液和单一铜绿微囊藻3种处理投喂铜锈环棱螺达15天，用ELISA法检测，得到了微囊藻毒素在螺体内的动态变化曲线。

Four substances including 2-methyl ethyl acetoacetate and barley straw extract distilled from bulrush as well as cetyl trimethyl ammonium bromide and isothiazo lintone were used for the comparison experiment in inhibiting the growth of Microcystis aeruginosa in various phases. Result showed that inputting chemicals in the lag phase has better effect than in the log phase, and adding in lag phase can effectively inhibit the algae growth. Although feeding allelopathic substance at log phase can lead to certain restraining effect, it cannot result in the effective inhibition of algae growth, which can be well achieved by feeding CTAB and isothiazo lintone below 10mgl/L during log phase.

研究用从芦苇中提取的2-甲基乙酰乙酸乙酯和大麦秸浸出液两种化感物质以及十六烷基溴化钱和异噻唑啉酮等四种药剂对不同生长期的铜绿微囊藻进行了对比抑制试验，结果显示在铜绿微囊藻生长的迟缓期投加试验药剂效果比在对数期投加效果都好，在迟缓期投加四种药剂，都有很好的抑藻效果；在铜绿微囊藻生长的对数期投加化感物质，虽然有一定的抑藻率，但效果较差，而在藻对数期投加10mg/L以下的CTAB和异噻唑啉酮能达到很好的水华抑制效果。

Front of the house to do two 34-hole drilling has set the pillar of the pillar set up above the small capsule known as a stick, if the three were to catch a small capsule, means that the owner is not going away, all three small bag, then pulled down Means that the owner at home, for the time being pulled out when a small capsule, a second small bag on board a white cloth, that is, men and women are masters of the family "made man movement", please visit again another day.

屋前做两根钻有三四个洞的定柱石，定柱石上面搭的棍子叫做丁囊，如果三个丁囊都搭上的话，意味着主人不在外出远地，三个丁囊都拔下来的话，意味着主人在家，暂时出去的时候就拔掉一个丁囊，如果第二个丁囊上搭有一条白布，就是，男女主人正在家中进行&造人运动&，探访者请改天再来。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。