四唑

An ionic liquid (1-ethyl-3-methylimidazolium tetrafluoroborate, 1E-3MI-TFB) modified micellar electrokinetic capillary chromatographic method was developed to separate and determine isoquercitrin, chlorogenic acid and quercetin.

建立了一种离子液体（1-乙基－3-甲基咪唑四氟硼酸盐，1E-3MI-TFB）修饰毛细管胶束电动色谱法分离测定异槲皮甙、绿原酸和槲皮素的方法。

Chinensis and Kuruma prawn M. japonicus increased at 12 and 48 hours post-inoculum, but decreased sharply after 36 and 72 hours post-inoculum respectively. LPS inducible NOS activity also decreased significantly with the time elapsed in WSSV infected shrimps. Results of WSSV detection with the Dig-DNA Probe dot blot hybridization test showed that WSSV of the two penaeid species couldn't be detected until iNOS started decreasing.

在此基础上，我们建立了对虾血细胞中诱导型一氧化氮合成酶的鉴定方法—硝基四氮唑蓝还原法和血细胞形态法以及酶活力的测定方法—L-瓜氨酸分析法和亚硝酸盐分析法并优化了鉴定和测量条件，结果显示：对虾血细胞在4℃下与L-精氨酸和LPS孵育8h以及当L-精氨酸浓度为2.5mmol l〓LPS浓度为100μg ml〓，Ca〓浓度为2.5mmol l〓时，iNOS活力的测定结果最佳。

Methods The cytotoxic effects of the four kind extracts of Livistona chinesis on two tumor cell lines cultured in vitro were tested by MTT assay.

采用四甲基偶氮唑盐比色法，观察蒲葵子提取物的各萃取部位对肝癌细胞HepG2和白血病细胞HL60增殖的抑制作用。

Methods32 Wistar male rats were used to induce focal cerebral ischemia according to Longa's method.

3 2只Wistar雄性大鼠，按照Longa的线栓法制作局部脑缺血模型，但在放入栓塞线时不结扎翼腭动脉，通过局部脑血流监测、动物行为学评分、脑含水量测定、红四氮唑染色和病理学检查等方法评价该模型的可靠性。

A series of newl naphthalenic sulfonated polyimides were synthesized using 1,4,5,8-naphthalenetetracarboxylic dianhydride, 2,2'-benzidinedisulfonic acid by one-step high temperature polymerization method. The sulfonated degree of SPI-X membranes was controlled through adjusting the molar ratio of BDSA to the non-sulfonated diamines.

采用1，4，5，8-萘四酸二酐、2，2′-联苯二胺双磺酸和APBA为单体，通过高温一步聚合法合成了含有噁唑环的新型磺化聚酰亚胺，通过调节二胺单体的比例控制磺化程度。

Results At different time and concentration,genistein had different effects on osteoblastic cell proliferation.

用四甲基偶氮噻唑蓝比色法检测细胞增殖，碱性磷酸酶活性测定检测细胞的分化。

Equilibrium constants for reactions of ZnTPP (parasubstituted zinc tetraphenylporphyrins) with ligands of Schiff base complex in CH2Cl2 and several other solvents have been measured by visible spectral techniques, and determined by the method of Rose-Drago.

用紫外-可见光谱和Rose-Drago数据处理方法对多种新型空间不对称含咪唑基团金属Schiff碱配合物与取代四苯基卟啉锌轴向配位反应平衡常数进行了测量。

A GC headspace injection method for the determination of residual organic solvents such as methanol, ethanol, acetonitrile, dichloromethane, ethylacetate, 4 methyl 2 pentanone, toluene, DMF, chloroform and tetrahydrofuran in tazobactam was established.

建立了气相色谱顶空进样法测定三唑巴坦中甲醇、乙醇、乙腈、二氯甲烷、乙酸乙酯、甲基异丁基酮、甲苯、N ，N′-二甲基甲酰胺、氯仿和四氢呋喃等有机溶剂的残留量。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

The effects of the application of modified suture on the total operation time, the common carotid artery blood occlusion time during the course of operation, the probability that suture entered the pterygopalatine artery and the success rate were observed. And the stability and the reliability of the model were evaluated by neurological deficit score, magnetic resonance image, triphenyltetrazolium chloride staining in all rats.

观察改良线栓对总体手术时间、术中颈总动脉血流阻断时间、线栓误入翼腭动脉几率和模型成功率的影响，并通过神经行为学评分、红四氮唑染色和磁共振检测等方法评价该模型的稳定性和可靠性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。