四唑

MethodTake quadri-combination therapy centering on Rabeprazole to treat positive pylorus spirillum digestive ulcer 84 cases.

方法采用雷贝拉唑为主的四联疗法治疗幽门螺旋杆菌阳性消化性溃疡84例。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

The influences of temperature and scan rate on the electrochemical characteristics of nitromethane were also investigated by using cyclic voltammetry.

采用循环伏安等测试方法，以铂微盘电极为工作电极，研究了硝基甲烷在离子液体1-正丁基-3-甲基咪唑四氟硼酸盐(BMImBF4)中的电化学行为，并探讨了温度和扫描速率等因素对硝基甲烷电化学特性的影响。

The antitumor effects of sesquiterpene lactones scabertopin and isodexyelephantopin from the whole plant of Elephantopus scaber were studied in vitro on SMMC-7721,Hela and Caco-2 cancer cell lines.

采用四甲基偶氮唑盐比色法和琼脂糖凝胶电泳法等研究地胆草倍半萜内酯化合物scabertopin(1)和isodeoxyelephantopin(2)在体外对SMMC-7721、Hela和Caco-2三种肿瘤细胞增殖的影响。

With L-DOPA as the starting chiral phenylalanine derivatives, construction of the skeleton of Phthalascidin was studied. Firstly, starting from L-DOPA and 3,4-dimethoxyphenylalanine, an amide 〓 was obtained, and a modeling cyclization reaction was carried out but failed to give the anticipated product.

二、对由苯丙氨酸甲酯衍生物通过Bischler-Napieralski反应合成四氢异喹啉—3—甲酯进行了研究，通过研究首次发现，生成苄基噁唑的反应是正常的B-N反应的竞争性反应，并从机理上给予了解释，所得出的结论具有一定的规律性，在杂环的合成方面具有一定的应用价值。

Objective: We detected the content of methylene chloride and diethylene oxide in Ceftizoxime Sodium using head-space GC method.

目的：建立一种气相色谱法测定原料药头孢唑肟钠中二氯甲烷和四氢呋喃有机溶剂残留量的方法。

Take quadri-combination therapy centering on Rabeprazole to treat positive pylorus spirillum digestive ulcer with quick cure and removing symptoms.

结论］雷贝拉唑为主的四联疗法治疗幽门螺旋杆菌阳性消化性溃疡溃疡愈合迅速，症状消失快。

Conclusion］Take quadri-combination therapy centering on Rabeprazole to treat positive pylorus spirillum digestive ulcer with quick cure and removing symptoms.

结论］雷贝拉唑为主的四联疗法治疗幽门螺旋杆菌阳性消化性溃疡溃疡愈合迅速，症状消失快。中国论文联盟http：//www.lwlm.com

Key words:digestive ulcer;Rabeprazole;quadri-combination therapy;pylorus spirillum

消化性溃疡；雷贝拉唑；四联疗法；幽门螺旋杆菌

The inhibition on the growth of CAOV3 was tested by flow cytometry and methyl thiazolyl tetrazolium assay, etc.

通过四甲基偶氮唑蓝比色法及流式细胞术等，观察转染 Rb基因的CAOV3细胞的生长情况。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。