四分染色体

The results showed that the number of chromosome, which was from the cultured seedlings of meristem tip and the rank seedlings of meristem tip, was not altered, both the number were 2n=16,the hereditary stability of callus and regeneration plant was poor; and the variation percentage of chromosome in callus was 43.4%, among which haploid accounted for 6.7%, triploid accounted for 2.5%,tetraploid accounted for 10%,pentaploid accounted for 4.2%,hexaploid accounted for 3.3%,septuploid accounted for 4.2%,octoploid accounted for 3.3%,dysploid accounted for 9.2%;the chromosome variation percentage of differentiation seedlings derived from callus was11.7%,among which haploid was 6.7%,triploid accounted for 1.7%,tetraploid was 3.3%.

结果表明，茎尖分生组织培养的幼苗及丛生苗遗传稳定，其染色体未发生倍性变异，均为2n=16；愈伤组织及其再生苗遗传稳定性较差，愈伤组织染色体数变异率为43.4%，其中单倍体占6.7%、三倍体占2.5%、四倍体占10%、五倍体占4.2%、六倍体占3.3%、七倍体占4.2%、八倍体占3.3%、非整倍体占9.2%；愈伤组织分化苗染色体变异率为11.7%，其中单倍体占6.7%，三倍体占1.7%，四倍体占3.3%。

Lines which were 2n=21"+1" and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group.

对导入赖草优质耐盐基因的株系Line15进行了C-分带、荧光原位杂交、SSR相结合的鉴定，确认Line15是一个二体异附加系，附加的一对染色体与小麦的第二同源群长臂、第三同源群着丝粒和第七同源群短臂密切相关，并确定了Line15的外源染色体C-分带特征带型，为目后鉴定Line15附加系的真伪提供了简便快捷的途径；对抗旱附加系Line17进行了荧光原位杂交和SSR检测，得出Line17也是一个二体异附加系，外源染色体与小麦的第四同源群短臂密切相关。

Chromosome number in all counted complete metaphases of Carassius auratus var. Dongtingking is 48. Analysis of chromosome can be divided into four sections according to the position of the centromere.

核型分析结果表明，翘嘴鲌的肾细胞染色体组是由48条染色体组成，染色体组型按着丝粒位置可分为四组，分别是中部着丝粒染色体、亚中部着丝粒染色体、端部着丝粒染色体。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。