囊鼠

Allantois and surrounding tissues of 8.5 day post coitum embryos, the hindhut and surrounding tissues of 9.5 dpc and 10.5 dpc embryos, the gonadal ridges and surrounding tissues of 11.5 dpc and 12.5 dpc embryos were collected and digested with 0.25%pancreatin-0.02%EDTA, then the cells were cultured in the plastic petridishes which are pretreated with 0.1% gelatine.

实验方法：取胚龄8.5 d胎鼠尾端尿囊及周围组织，取胚龄9.5~10.5 d胎鼠后肠及周围组织，取胚龄11.5 d，12.5 d胎鼠生殖嵴及周围组织，分别经0.25%胰酶-0.02%EDTA消化并接种于0.1%明胶包被的培养皿中。

The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养，并应用免疫细胞化学方法，对培养细胞进行染色标记和观察，系统了解哺乳动物听觉细胞发生、发育过程，以及这些细胞在体外的增殖和分化过程，旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源，发现细胞生长的前体细胞。

Then replate them onto feeder layer culture system (containing leukemia inhibitory factor) in order to passage and expand. Subsequently we identified the expanding cells by alkaline phosphatase staining, karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs.

以分离培养的细胞作为供体细胞，近交系C57BL/6J为受体囊胚供者，采用囊胚注射法构建嵌合胚，体外恢复培养后移植进动情期子宫中，结果生出了黑白杂合的嵌合鼠。

OBJECTIVE: This study aimed to investigate the immunological rejections of APA microencapsulated chromaffin cells transplanted into rat anterior chamber of eyes and tendon of feet, and to evaluate the immunoisolated effect of microencapsulation.

目的：观察海藻酸钠-多聚赖氨酸-海藻酸钠微囊化嗜铬细胞移植到大鼠眼前房和足胝部的免疫排斥反应，评价微囊化技术的免疫隔离作用。

To observe analgesit effects of xenogeneic bovine chromaffin cells and protective effects of the alginate-polylysine-alginate microcapsule.

采用微囊化牛嗜铬细胞异种移植于大鼠和癌痛病人脊髓蛛网膜下方法，观察BCC镇痛作用和海藻酸钠-聚赖氨酸-海藻酸钠微囊对异种移植物的免疫保护作用。

Moreover, two kinds of microcapsules were retrieved to compare their morphologies and pericapsular fibrosis after transplantation in SD rats for different times.

SD大鼠体内移植试验对比不同回收时间点两种微囊的完整性和囊周纤维化程度。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

The study on the pathogenesis of the growth of the experimental saccular aneurysms in rats Objective To study the possible pathophysiological mechanisms of the growth of the experimental saccular aneurysms in rats.

大鼠实验性囊性动脉瘤生长塑形机制的研究目的探讨在囊性动脉瘤生长塑形过程中可能存在的血管生物学方面的病理生理机制。

After 2, 4, 6, and 8 weeks of feeding, 2% fluorescein sodium was dropped into rat conjunctival saccus and breakup time of tear film was observed by cobalt blue light scanning under slit lamp microscope. Then the rats were executed after anesthesia.

分别饲养2，4，6，8周后，用2%荧光素钠滴入大鼠结膜囊内，瞬目后在裂隙灯显微镜下用钴蓝光扫描照射观测大鼠的泪膜破裂时间，然后麻醉下颈椎脱臼法处死大鼠。

Methods 30 Sprague-Dawley rats were intra-abdominally injected 25 metacercarias and randomly divided into 6 groups.

将35只清洁级Sprague-Dawley雄性成年大鼠随机分成7组，其中6组每只鼠腹腔注射斯氏狸殖吸虫囊蚴25个，分别于感染后1w、3w、5w、7w、11w、15w、取大鼠外周全血，采用流式细胞术检测CD4、CD8淋巴细胞的百分率。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。