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The blastula formation whose embryos were from different sources was compared and the blastomere number, quality classification and the relationship between the parity of the blastomere number and the blastula formation at d 3 were studied.

比较不同来源胚胎囊胚形成情况和d3胚胎的卵裂球数、质量分级等;并利用废弃胚胎囊胚形成情况对体外受精妊娠结局进行预测。

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

结果:与无上皮细胞相比,共培养明显促进了小鼠桑椹胚率(71.3%和61.2%,p.05),囊胚率(50.0%和39.7%,p.05),囊胚孵化率(34.8%和24.3%,p.05)和胚胎总细胞数(44.1和35.6,p.05)。结论:子宫内膜上皮细胞能够促进小鼠胚胎的体外发育,对早期胚胎的体外发育环境具有优化作用。

Then replate them onto feeder layer culture system (containing leukemia inhibitory factor) in order to passage and expand. Subsequently we identified the expanding cells by alkaline phosphatase staining, karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs.

以分离培养的细胞作为供体细胞,近交系C57BL/6J为受体囊胚供者,采用囊胚注射法构建嵌合胚,体外恢复培养后移植进动情期子宫中,结果生出了黑白杂合的嵌合鼠。

Three kinds of embryos' chromosome, including goat fertilization embryo, goat-goat cloned embryo and goat-rabbit cloned embryo were analyzed, The results showed that all of them have 60 chromosomes with same character, all were telocentric chromosome, besides the X-chromosome is the second larger telocentric chromosome.

经对超排获得的山羊受精胚、山羊同种克隆胚和羊-兔异种克隆胚的囊胚期胚胎染色体分析表明,三种来源的胚胎其染色体数目均为60条,且所有常染色体均为端部着丝点染色体,X染色体为第二大的端部着丝点染色体,说明山羊同种克隆胚和山羊-兔异种克隆胚均为山羊的核型,是供体细胞核在不同胞质中的重新编程的结果。4。

In fish and amphibia, the transition from maternal gene transcription to zygotic transcription happens during the time of midblastula formation, this stage is named by midblastula transition, at this period, embryo cell cycle composition changes, it is the first time to G1 time appearance, the cell cycle timelimit lengthens and the cell division synchronism loses, the large quantities of zygotic gene transcription starts.

鱼类和两栖类由母型调控向合子型调控过渡发生在中囊胚形成阶段,称为中囊胚过渡(midblastula transition,MBT),胚胎细胞周期时相的组成在此期间发生变化,首次出现G1期,周期时限延长,细胞分裂的同步性丧失,大批合子型基因活化转录也在此时开始。

In lungs, AQP1 was abundant in peribronchiolar capillaries, postcapillary venules, bronchiolar basal membrane and alveolar epithelial cells.

结果表明,托吡酯在给药剂量范围内(0.068μg-6.8μg/鸡胚)可抑制鸡胚尿囊膜的血管生成,剂量为0.68μg/鸡胚和6.8μg/鸡胚时抑制效果明显,最高抑制率达33.5%。

The infolding of a portion of the outer layer of a blastula in the formation of a gastrula.

由于细胞向内移动囊胚形成胚囊或肠胚的过程。

The process of forming a gastrula.

由于细胞向内移动囊胚形成胚囊或肠胚的过程。

However, cell counts and inner cell mass to trophectoderm ratios of the embryos exposed to elevated temperature were not different from controls, suggesting that the developmental potential of the embryos which survive to blastocyst stage are not overtly impaired.

然而,处理组牛胚在温度升高过程中,发育到第9天的囊胚数,细胞数及内细胞团与滋养外胚层比率在统计学上与对照组之间没有差异。由此揭示,升高温度对牛囊胚期胚胎的发育没有明显损害。

The cleavage rate of 2-cell stage was 50%, but few embryos developed into morulas and blastocysts (2%). More than 130 embryos at 2-4-cell stage were transferred into oviduct of 13 synchronized foster rabbits and 2 mice. No fetus or implantation sites were observed in both recipients.

其2—细胞胚胎的分裂率可达50%,但发育至桑椹胚和囊胚阶段的胚胎数目大大减少,只有2%的囊胚发育率。13只受体兔共移植重构胚胎121枚,结果未发现产仔或死胎,仅看到有的兔子宫有於血状。

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