囊胚的

Observing the mature embryo sac of the mutant: It was found thatthe embyo sac appeared in typical polygonum type and more than 90% of the embryo sac hadintegral inner structure. They had egg apparatus, two polar nucleus and three antipodal cells , and their position in the embryonic sac was normal .

幼穗分化、成熟胚囊观察发现，90％以上的胚囊具有完整的内部结构，有卵器、两个极核及多个反足细胞，且位置正常，胚囊呈现典型的蓼型结构；同时还观察到雌、雄器官的发育是同步的。

Most of ovaries in the mutant were without embryo sac,and the sac full of par enchyma cells.

大多数子房不具真正的胚囊，"胚囊"中充满了薄壁细胞。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

The double embryo sac is a new type of variation in rice,chartered with a large embryo sac inlaid with a small embryo sac,overlapping in theirmicropyle part,and with the eggs far from micropyle part.

双套结构胚囊是一种新发现的胚囊，主要特点是含有大小两个相嵌的胚囊和远离珠孔端的卵细胞。

In order to discuss the optimal condition in which GPMV proliferates in chicken embryo and its pathogenicity, GPMV of different titer was inoculated into 911-day chicken embryo and the allantoic fluid was harvested after 36, 48, 60, 72 and 84 h, the HA titer was measured as well.

为了探讨鹅副粘病毒在鸡胚中增殖的最佳条件及其致病性，将不同血凝滴度的鹅副粘病毒分别接种于9~11日龄鸡胚尿囊腔，收获孵化36、48、60、72和84 h的鸡胚尿囊液，测定血凝滴度。

The embryological studies of strawberry showed that the ovule of strawberry were hemitropous, the ovule had the obvious hypostace structure, the type of its embryo sac was Polygonum, the embryo sac was long and narrow, the type of development of embryo was the Geum type of the Asterad type, the endosperm was Nuclear type and the somatic embryo sacs happened The integral transparency technique combined with the technique of microdissection was used in this experiment The combination was an innovation in the techniques of section.

对草莓胚胎学的研究结果表明：草莓胚珠横生、胚珠具明显的承珠盘结构、胚囊发育为蓼型、胚囊狭长、胚胎发育为紫菀型水杨梅类型、胚乳核型、并有体细胞胚囊的产生。试验中采用了多种制片方法，其中将整体透明技术和显微解剖技术相结合的方法是在制片技术上的一次创新。

METHODS: Constructing the CAM model by exposing CAM on the shell subpoint between 2 vitelline veins and generating an air chamber after 5 days of incubated with aseptic incubator at 37℃.

将鸡胚消毒后，置于37℃无菌恒温箱中孵育5 d，在距胚头1 cm两条卵黄静脉之间的卵壳投影部位磨切暴露尿囊膜，制成假气室，用无菌滤纸封闭窗口，制成鸡胚尿囊膜模型，备用。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示，在卵母细胞和8细胞期胚胎，基因calm3和trm112l的表达量无显著性差异，而在囊胚期的表达量与前两个时期的表达量存在显著性差异，推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控，提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。