囊胚的

However, the blastocyst rate of embryos cultured in TE was significantly higher than mWM and CZB.

用等量的果糖代替培养液mWM中的葡萄糖，所形成的培养液mWM3能使82%的胚胎发育到囊胚期。3。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

Embryonic stem cell has the capability of immortalization and pleuripotent differentiation.

胚胎干细胞源于着床前的囊胚内细胞团或早期胎儿原始生殖细胞的一类未分化的多能性干细胞，具有无限增殖和多能分化的能力。

Embryonic stem cells derived from inner cell mass of mammalian blastocyst-stage emyst-stage embryos and primordial germ cells are totipotent cells that can be cultured in virto.

胚胎干细胞是从哺乳类动物的囊胚内细胞群或原始生殖嵴细胞分离、体外培养获得的具有发育全能性的细胞。

The method of vitrification cryopreservation of mouse 2-cell embryos was studied. Several vitrification solutions named EFS30, EFS40, EDFS30 and EDFS40 were prepared. The solution contains ethylene glycol, Ficoll, sucrose and DMSO. Straw method was used to vitrify and cryopreservate in vivo mouse 2-cell embryos at room temperature (20℃ or 25℃). OPS method was used to vitrify the embryo at 25 ℃ or 37℃.

本试验以毒性低、渗透性较强的乙二醇和DMSO为主体，添加聚蔗糖、蔗糖配制的玻璃化溶液(EFS30、EFS40、EDFS30和EDFS40)，在室温(20℃和25℃)下利用细管法，以及在25℃和37℃恒温台条件下OPS法对小鼠2-细胞胚胎进行玻璃化冷冻保存，并对解冻后胚胎的体外培养、囊胚细胞计数及胚胎移植技术进行了研究。

Three types of cells were transferred into perivitelline space under zona pellucida of rabbit oocytes respectively.

用体外培养14代和24代的小鼠胚胎干细胞构建的异种重构卵的卵裂率分别是88.5％和82.1％(P＞0.05)，囊胚发育率分别是16.7％和15.4％(P＞0.05)。

According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere.

摘要从哺乳动物早期胚胎体外培养发育阻断机理和早期胚胎的体内环境入手，阐述了胚胎体外培养各细胞阶段胚胎对营养物质的需求，寻求合理的体外培养条件和方法，以便提高体外胚胎早期的囊胚发育率。

The purified immunoglobulins were effective in the inhibition of endothelial cell adhesion, proliferation and angiogenesis in chick chorioallantoic membrane in vitro, and in anti-tumor activity by the adoptive transfer in vivo.

将该抗体纯化后，纯化的免疫球蛋白可以有效地抑制体外内皮细胞的粘附、增殖和鸡胚绒毛尿囊膜的新生血管形成，体内过继输入治疗亦可引起抗瘤效果。

These ES cells were introduced into host embryos from outbred KMW though blastocyst injection. Using GFP, the attractive genetic marker, we have been able to follow the fate of ES cells in living embryos and to observe directly the contribution of these cells to mouse embryos. Totally, four GFP-expressing 'green' chimeric mice expressing GFP were obtained.

为研究嵌合体动物中供体胚胎干细胞在宿主胚胎发育中的走向和定位，同时探讨绿色荧光蛋白基因作为报告基因在转基因动物制作中的应用价值，本研究将pEGFP-N1基因导入小鼠ES-D3细胞系，得到稳定表达GFP的胚胎干细胞亚系ES-D3-GFP，通过对昆明小鼠的囊胚腔注射，获得了4只表达绿色荧光蛋白的嵌合体小鼠。

Results shows that the earlier the treatment starts, the more the decrease of gsc expression in late blastula, the fewer the number of somites at 24 h, and the more severe the defects in axial mesoderm. This suggests that maternal TGF-βsignals are activated immediately after fertilization and such activation continues before the onset of zygotic gene expression.

为了确定斑马鱼中母源TGFβ信号的激活时间，本研究用50μM SB-431542在中囊胚过渡期前(即合子基因开始表达之前)的不同时期处理胚胎，随后观察胚胎形态和检测分子标记的变化。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。