囊胚

The zygote, by cell division, grows into a morula an then into a blastocyst.

通过细胞分裂，受精卵发育成桑葚胚，然后发育成囊胚。

Furthermore, when glucose was introduced into the CZB medium on the third day of the culture, the development rates of over morula and blastocyst(66.7%,28.1%) were improved significantly.

进一步，于培养的第3 d在CZB培养液中加入葡萄糖，其桑椹胚以上及囊胚发育率明显提高（66.7%、28.1%）。

The survival rates of early blastocyst treated with trehalose and sucrose, were 79.1%, and.77.1%, respectively; and the survival rates of morula were 46.3%, and 51.6%, respectively.

用海藻糖和蔗糖处理的小鼠早期囊胚存活率分别为79.06％和77.07％；桑椹胚分别为46.31％和51.63％。

The survival rates of mice early blastocyst and morula treated with 0.5M trehalose were 61.8% and 95.6% respectively; and treated with 0.25M trehalose were 35.5% and 67.2% respectively.

小鼠桑椹胚和早期囊胚用0.5M海藻糖处理后存活率分别为61.84％和95.57％；用0.25M海藻糖处理后存活率分别为35.50％和67.22％。

Among the 20 samples of IGF- 1 group, a 8-cell stage embryo, a morula and two blastula were found as positive by the red colour appearance .

IGF上的表达 2～8细胞胚胎 18个，桑堪胚 1个，囊胚 2个，共计 ZI个胚胎用于＊F2 检测，阳性表达14个，其中强阳性表达7个，弱阳性表达7个，分布于各阶段胚胎，在胚胎细胞中呈现深、浅程度不同的橘红色染色。l级和 2级胚胎 IGFZmRNA的阳性表达差异有显著性。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测，最早在肢芽期胚胎中检测到2A3阳性信号；在膜内无节幼体期，2A3阳性血细胞平均直径约7μm，聚集成簇；在无节幼体Ⅰ期，血细胞形态更加完整清晰，平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现，这些细胞同样成簇存在，在其细胞质外围可见点状荧光。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P＜0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P＜0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB，可明显提高双原核的形成率（38.9％vs 15.1％，P＜0.05）和桑椹胚/囊胚发育率（40.7％vs 26.8％，P＜0.05），但对其激活分裂率和总原核形成率无明显影响（P＞0.05）。

There were no significant difference amongedifferent adult skin fibroblasts on the cleavage and development of reconstituted embryos.

研究发现来自不同个体的皮肤成纤维细胞所构建的重组胚，卵裂率和囊胚发育率无差异。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

总之，我们的研究表明，DET玻璃液冷冻方法是简单、经济而又高效的，冷冻小鼠各阶段胚胎都能获得较高的存活率，其中受精卵的EDT30二步法1分钟平衡组(66％)、桑椹胚的EDT30二步法1分钟平衡组(94％)和EDT40二步法0.5分钟平衡组(94％)、囊胚的EDT30二步法1分钟平衡组(91％)和EDT40一步法1分钟平衡组(91％)冷冻效果最好。

BACKGROUND: Embryonic stem cells develop from early blastular inner cell mass. Under proper condition, ESCs can maintain undifferentiated state in vitro, normal diploid nuclear type, and proliferative potential. In addition, ESCs possess multi-directional differentiation capacity and can differentiate into all sorts of cells of three germ layers.

背景：胚胎干细胞来源于发育早期囊胚的内细胞团，适当条件下能在体外维持未分化状态、正常二倍体核型及无限增殖能力，且具有多向分化潜能，能够发育分化成机体3个胚层的所有类型细胞。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。