囊胚

Objective To investigate the effect of different splitting solutions and decompaction treatment on the bisection of mouse embryos at morulae and blastocysts stage and their subsequent transfer after cultured.

目的 研究不同分割液和分割前胚胎去致密化与否对昆明白系小鼠的桑椹胚和囊胚的徒手分割以及分割后胚胎移植的影响。

A independent life individuality is finally formated from singlecell ferti1ized egg throngh egg cleavage、morula stage、blastocoele stage、protogaster stage and orange etc, and concerned in major basal questions of development and gene expression.

单细胞的受精卵通过卵裂、桑椹胚、囊胚、原肠胚及器官的发生等，最后形成一个能独立生活的个体，涉及发育和基因表达的许多基本问题。

Result The embryonic development of Silnrus asotus in Dongting Lake was divided into 7 stages, namely, blastoderm stage, cleavage stage, blastula stage, gastrula stage, neurula stage, organogenesis stage and pre-hatching stage.

结果］洞庭黄鲶的胚胎发育过程可分为胚盘期、卵裂期、囊胚期、原肠期、神经胚期、器官形成期和出膜期7个阶段。

The cells of blastomere randomly distributed either embryonic parts or extraembryonic parts of blastocyst.

2-细胞胚胎卵裂球随机分布在囊胚的胚胎部分和胚外部分。

GV oocytes with normal shape were not obtained in the group which was stored at 20℃ for 24 h and at 25℃ for 14 h; A blastula rates (64%) of all groups in average was gotten in culturing 2-cell in vitro, and there is no significant difference in temperature and time of storage. After transplantating some of the 2-cell embryos which were obtained from GV oocytes stored at 15'C for 8 h, normal infant mice could be obtained.

在20℃下保存24h和25℃下保存14h时，难以获得形态正常的GV期卵；体外受精获得的2细胞期胚经体外培养，总体上有64%的胚胎发育至扩张囊胚，未见有保存温度和保存时间的显著影响，且利用在15℃保存8h后的GV卵获得2细胞期胚的移植获得正常新生小鼠。

After lOmin delay only 81.2% pronucleus developed to blastula, significantly lower than control (93.2%).

滞留20min不引起原核胚、2-cell胚胎和桑椹胚以及囊胚的死亡。

Here were expression of TGFαand EGFR mRNA in unmatured oocyte, matured oocyte, embryo and the expression of TGFαand EGFR in matured oocytes were comparatively more abundant than that in unmatured oocytes.

GFαmRNA的量在8细胞期下降到最低水平，并随着胚胎基因组的激活胚胎开始转录TGFα，使TGFαmRNA表达总量保持在一定水平，持续到桑椹胚期；EGFR mRNA的量在胚胎发育到桑椹胚期下降到最低，而囊胚期胚胎中的整体表达量又上升。

With the increase of the 1254 dosages, in the further development process of 2-cell embryo, there still occurred the development relayed and degeneration, so that the development rate of morulae and blastocysts decreascd significantly.

在受精卵的进一步发育中，随著公鼠染毒剂量的增加，发育到桑椹胚和囊胚的比例显著降低，发育延迟胚及发育中退化变性胚比率显著升高。

The aim of the present study was to isolate and culture ESCs and EGCs from goat embryos and fetuses of different development stage, and to compare some factors influencing the efficiency of establishing those stem cell lines by adopting the methods of mouse and human. The results obtained were as follows: 1. A total of 46 Guanzhong dairy goats were superovulated with a result of total 307 embryos including 39 embryos of 4-cell to 16-cell, 128 morulae, 89 blastocysts and 51 hatched blastocysts. In the study, we obtain 6.67 embryos from per goat.

本研究选用关中奶山羊胚胎和胎儿为试验对象，参照小鼠和人ESCs和EGCs建系的方法，系统比较了不同培养条件和传代方法对分离培养山羊ESCs和EGCs的影响，主要获得以下结论： 1本试验共超排处理了46只/次关中奶山羊，共获得胚胎307枚，其中4~16细胞胚胎39枚，桑椹胚128枚，囊胚89枚，孵化胚51枚，平均每只山羊获得6.67枚胚胎。

Methods C 57BL/6J strain female mice mated with KM male mice, after seeing plug 3.5 da ys the compacted morulae and blastocysts were collected and bisected microsurgic ally by using special knife, obtained half embryos were cultured and observed.

C57BL/6J雌性小鼠与KM雄性小鼠交配，见栓3.5d采集小鼠晚期桑椹胚和早期囊胚，应用显微手术刀直接分割法进行胚胎二等份分割，所得半胚体外培养，观察其发育情况。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。