囊胚

There are significantly differences on H_2O_2 levels between embryos in vivo and cultured in vitro on the early 2-cell stage and late 2-cell stage, but there are no significantly differences among embryos those on the 4-cell stage, morula and blastula using SPSS statistics software.

我们测量的结果是体内2-细胞早期、2-细胞晚期、4-细胞期、桑椹胚、囊胚胚胎的EB荧光密度分别是52.92，50.77，48.84，39.96和30.36；体外2-细胞早期、2-细胞晚期、4-细胞期、桑椹胚、囊胚胚胎的EB荧光密度分别是74.85，82.62，82.98，81.28和54.09。

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

结果：与无上皮细胞相比，共培养明显促进了小鼠桑椹胚率（71.3%和61.2%，p.05），囊胚率（50.0%和39.7%，p.05），囊胚孵化率（34.8%和24.3%，p.05）和胚胎总细胞数（44.1和35.6，p.05）。结论：子宫内膜上皮细胞能够促进小鼠胚胎的体外发育，对早期胚胎的体外发育环境具有优化作用。

These observations show that reprogramming is easier in interspecific embryos reconstructed with ES cells than that reconstructed with somatic cells, and that ES cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. Oocytes were reconstructed with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed oocytes were analyzed. The interspecific reconstructed ES-rabbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electr

以上结果显示，6－DMAP能增加胚胎干细胞异种重构卵的卵裂率，对重构卵囊胚的发育率影响不大；高培养代数和低培养代数的胚胎干细胞用于异种核移植不影响异种重构卵的卵裂率和囊胚发育率；用胚胎干细胞作为供核细胞；比体细胞作为供核细胞所构建的异种重构胚更容易进行重编程，并且胚胎于细胞指导异种克隆胚胎正常发育的能力强于体细胞。

Then replate them onto feeder layer culture system (containing leukemia inhibitory factor) in order to passage and expand. Subsequently we identified the expanding cells by alkaline phosphatase staining, karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs.

以分离培养的细胞作为供体细胞，近交系C57BL/6J为受体囊胚供者，采用囊胚注射法构建嵌合胚，体外恢复培养后移植进动情期子宫中，结果生出了黑白杂合的嵌合鼠。

The fusion rate and blastocyst rate of intra-species SCNT embryos was 78.67 (557/708); 56.29%(264/469), that of sub-species or inter-species SCNT embryos were: boer goat 78.18%(541/692); 33.90%(40/118), bovine 70.53%(146/207); 22.52%(25/111), tahr 53.51%(61/114); 5.26%(3/570), panda 79.82%(1159/1452); 8.35%(75/898) and human 68.76%(317/461); 5.41%(16/296), respectively.

种内体细胞核移植胚的融合率和囊胚发育率分别为78.67%(557/708)和56.29%(264/469)；亚种间或种间体细胞核移植胚的融合率和囊胚发育率分别为：波尔山羊78.18%(541/692)、33.90%(40/118)，牛70.53%(146/207)、22.52%(25/111)，塔尔羊53.51%(61/114)、5.26%(3/570)，熊猫79.82%(1159/1452)、8.35%(75/898)，人68.76%(317/461)、5.41%(16/296)。

The infolding of a portion of the outer layer of a blastula in the formation of a gastrula.

由于细胞向内移动囊胚形成胚囊或肠胚的过程。

The process of forming a gastrula.

由于细胞向内移动囊胚形成胚囊或肠胚的过程。

However, cell counts and inner cell mass to trophectoderm ratios of the embryos exposed to elevated temperature were not different from controls, suggesting that the developmental potential of the embryos which survive to blastocyst stage are not overtly impaired.

然而，处理组牛胚在温度升高过程中，发育到第9天的囊胚数，细胞数及内细胞团与滋养外胚层比率在统计学上与对照组之间没有差异。由此揭示，升高温度对牛囊胚期胚胎的发育没有明显损害。

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h转为正常培养24h，不影响卵母细胞的成熟和孤雌激活能力，并且CHX抑制后再成熟的卵母细胞经孤雌激活发育到囊胚的比例与对照组卵母细胞相似。8、体外受精后，CHX抑制后再成熟卵母细胞的多精受精现象显著增加，发育到桑椹胚/囊胚的比例显著低于对照组。9、CHX抑制过程中皮质颗粒仍能发生迁移，但是CHX抑制会对皮质颗粒的迁移造成不可恢复的损伤，使再成熟的卵母细胞内皮质颗粒不能完全迁移。10、CHX抑制对山羊卵母细胞α-微管和微丝都没有影响，无论是抑制后处于生发泡期的卵母细胞，还是抑制后再成熟的卵母细胞，微管和微丝的分布都与对照卵母细胞相似。

The average cell number of each embryo were significant differences between 12-24 hr and 36-48 hr treatment and 0-72 hr treatment of early blastula; the cleavage index of each embryo were significant differences between 0-12hr and 24-36 hr treatment and 0-72 hr treatment of the hatching of blastula; embryo cell number and cleavage of other treatments were no differences.

早期囊胚的12-24h处理组和36-48h处理组的每一胚胎平均细胞数与0-72h处理组有显著差异；扩张囊胚0-72h处理组的分裂指数与0-12h处理组和24-36h处理组有显著差异，其它各组的胚细胞数和分裂指数均无差异。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。