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The mature embryo sac fertility, formation and development of embryo sac of 141 accessions of O. rufipogon Griff. collected from the six sampling sites in Gaozhou, Guangdong Province were studied by using the technology of whole-mount stain-clearing confocal laser scanning microscopy.

利用整体染色激光扫描共聚焦显微镜术,对采自广东省高州市6个地点共141个编号的高州普通野生稻的成熟胚囊育性和胚囊形成发育特点等进行研究。

Abnormal phenomena in different periods of embryo sac development, including degeneration of megaspore, embryo sac abnormality during the periods of two to eight nucleate etc., were found in the plants with low seed setting rate.

对一些结实率偏低材料的研究,发现在胚囊发育过程的不同时期存在一些异常现象,包括功能大孢子退化,二至八核胚囊发育异常等。

But,some functional cells containdistinct starch grains like mature megaspore and single nucleate embryo sac which isin the station before division.

同样,从单核胚囊分裂开始到八核胚囊刚形成也未观察到淀粉粒。

Embryo sac A large oval thin-walled sac in the nucellus of flowering plants in which egg fertilization and subsequent embryo development occurs.

胚囊:是存在于显花植物胚珠珠心中的一个大型椭圆状的囊状结构,卵细胞受精和随后的胚胎发育均在这里进行。

Pox formation increased from 13.3%-20% to 33.3%-40% after passaging through 9 days old chicken embryo. Typical virus particles were observed under transmission electronic microscope in ultrathin sections of pathological tissues, allantochorion membrane of chicken embryos and affected cells.

用待检病料感染的BHK-21细胞培养物接种9日龄鸡胚绒毛尿囊膜,随着传代次数的增加,痘斑病变的出现率从13.3%~20%上升至33.3%~40%;用山羊痘病变皮肤、鸡胚绒毛尿囊膜和感染细胞进行超薄切片,在电子显微镜下可以观察到典型的山羊痘病毒粒子。

The result showed that the HA titer of virus in allantoic fluid reached its maximum 1:256~1:512 in the condition that the 9-day chick embryo received 50 times dilution of 1:64 GPMV and cultured for 48~72 h in 37℃.

结果表明将血凝价为1:64的鹅副粘病毒稀释50倍后,接种9日龄鸡胚,37℃培养48~72 h获得的尿囊液血凝价可达1:256~1:512,平均尿囊液量可达9.4 mL/胚。

Past study re-sults had indicated that MMC in the apomictic type could at most develop to te-trad stage.We observed that the sexual embryo sac of"Pingyi Tiancha"coulddevelop into 4-nuclei embry sac.

前人只观察到无融合生殖类型的大孢子母细胞至多能发育到四分体时期,而我们观察到平邑甜茶的有性胚囊能发育到四核胚囊。

To understand the degeneration of unfertilized aposporous embryo sac in an aposporous species Pennisetum squamulatum, the central cell in the unfertilized embryo sac was characterized ultrastructurally.

为理解植物无孢子生殖胚囊未受精条件下的退化,对无孢子生殖植物非洲狼尾草未受精成熟胚囊中央细胞退化做了细胞形态学研究。

METHODS: The different combinations of the biofilm were put on the surface of chick embryo chorioallantoic membrane.

将制备的生物膜置于正对观察窗的鸡胚绒毛尿囊膜表面的血管最少处,然后贴在鸡胚绒毛尿囊膜表面上,封口膜封闭假气室,继续孵育。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示:未妊娠时,泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达,波形蛋白在子宫内膜基质细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,牦牛子宫任何部位均不表达角蛋白7;妊娠30天左右时,泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达,波形蛋白在子宫内膜基质细胞和内胚层细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,角蛋白7在尿囊细胞内表达,偶尔在腔上皮细胞的细胞核边缘表达;消化法进行原代培养时,组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞;分离得到的子宫内膜基质细胞活率达90%以上,并可在体外传代7次以上;分离得到的子宫内膜腺上皮细胞活率可达85%以上,并可在体外传代5次以上;RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长,维持子宫内膜基质细胞正常生长的FBS添加量为20%,维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

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