囊胚

Methods Mouse endometrial epithelial cells were isolated and cultured,then it was cocultured with mouse embryos fertilized in vitro.

对小鼠子宫内膜上皮细胞进行分离、培养鉴定，然后与体外受精的小鼠胚胎进行共培养，观察共培养对小鼠胚胎卵裂率、孵化率以及囊胚期胚胎总细胞数的影响。

We used the medaka as a fish model for the development of ES cell technology. We have established feeder cell free culture conditions and obtained several ES cell lines from midblastula embryos.

我们以青鱂作为建立鱼类ES细胞技术的模式，通过建立并应用无滋养层细胞的培养条件，获得了来自中期囊胚的ES细胞系。

Both static groups produced a significantly higher proportion of blastocysts and morulas and a lower proportion of abnormal and eight-cell embryos compared with those of high flow rate groups.

与高流率组比较，静态组产生明显较高比率的囊胚及桑葚体，和较低比率之不正常及八细胞胚胎。

The proportion of embryos developing to the blastocyst stage was not significantly different between two systems, however the static microdevice environments produced significantly more morulas than did control groups.

胚胎发育成囊胚阶段在两个系统之比率并无明显差异，然而在静态微装置环境比对照组产生明显较多桑葚体。

But the latter resulted in significantly more zygotes extruded PBl and developed to blastocyst stage after parthenogenetic activation.

结果发现，成熟液中添加促卵泡素组的卵裂率(74.3％)低于人绝经促性腺激素组(78.6％，P＜0.05)，但是第一极体排出率和孤雌激活后的囊胚率都显著高于hMG组(P＜0.05)。

Results shows that the earlier the treatment starts, the more the decrease of gsc expression in late blastula, the fewer the number of somites at 24 h, and the more severe the defects in axial mesoderm. This suggests that maternal TGF-βsignals are activated immediately after fertilization and such activation continues before the onset of zygotic gene expression.

为了确定斑马鱼中母源TGFβ信号的激活时间，本研究用50μM SB-431542在中囊胚过渡期前(即合子基因开始表达之前)的不同时期处理胚胎，随后观察胚胎形态和检测分子标记的变化。

Early cleavage rate,embryo quality,the rate of blastocyst,implantation and pregnant rates from these zygotes were observed,and the transferred embryos were selected according to the pronuclei orientation and early cleavage time.

根据受精卵原核构型的不同分为四组，结合构型类型及早期卵裂与否进行选择性移植，通过观察优质胚胎率、囊胚形成率、种植率和怀孕率，来评估合子原核构型和早期卵裂与胚胎发育潜力的关系。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Subsequently cellular become divided brings about a series of " kind the formation of bursa embryo ".

随后的细胞分化则导致一系列&类囊胚&的形成。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。